Propargyl-PEG10-acid is a propargyl PEG linker with a terminal carboxylic acid. The terminal carboxylic acid forms amide bonds with primary amines; activators (EDC or HATU) will be needed. The propargyl group can react with azide-bearing compounds to form stable triazole bonds. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG10-acid is a propargyl PEG linker with a terminal carboxylic acid. The terminal carboxylic acid forms amide bonds with primary amines; activators (EDC or HATU) will be needed. The propargyl group can react with azide-bearing compounds to form stable triazole bonds. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Saxitoxins (STXs) are naturally occurring alkaloids produced by some marine dinoflagellates and by strains of various species of freshwater cyanobacteria. Saxitoxin is one of the prevalent paralytic shellfish toxins (PSTs). It belongs to a family of potent neurotoxins with a molecular weight around 300 Da. Saxitoxin and its derivatives are alkaloids composed of a tetrahydropurine ring system with a highly polar guanidinium group. Due to their significant toxicity, saxitoxins are closely monitored in marine environments where they can accumulate in the food chain during harmful algal blooms (HABs). An action level of 800ppb, or 80ug per 100grams of shellfish, has been established.
Screening of saxitoxin in shellfish samples as low as 50ppb in sample
Format: 25 tests (12 tests, 12 controls)
Filters, syringes, extract collection tubes, buffers
Run Time: 30 Minutes
This kit provides a rapid spin column method for the purification of total DNA from a broad spectrum of bacteriophages propagated in bacteria grown in liquid cultures. The DNA is isolated without the use of phenol, chloroform or cesium chloride banding procedures. The spin-column based procedure is rapid and can be completed in less than 45 minutes. The kit is highly efficient for processing small volumes of phage supernatant (500 µL – 1 mL) and with the optional DNase and Proteinase K treatments phage DNA yields are maximized while host DNA contamination is minimized. Purified total phage DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), sequencing, cloning, Southern Blot and more.
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| Kit Specifications | |
| Column Binding Capacity | 50 µg |
| Maximum Column Loading Volume | 650 µL |
| Size of DNA Purified | All sizes |
| Maximum Amount of Starting Material | 1 x 1010 pfu/mL enriched phages |
| Average Yield* | 3-15 µg DNA from 106-1010 pfu/mL of enriched phages |
| Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 46800 (50 preps) | Cat. 46850 (100 preps) |
|---|---|---|
| Lysis Buffer B | 40 mL | 2 x 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Buffer B | 8 mL | 2 x 8 mL |
| Spin Columns | 50 | 100 |
| Collection Tubes | 50 | 100 |
| Elution Tubes (1.7 mL) | 50 | 100 |
| Product Insert | 1 | 1 |
Herpes Simplex Virus 2 (HSV-2) is a member of the herpes virus family, Herpesviridae. HSV-2 has a relatively large double-stranded DNA genome. HSVs are primarily transmitted by sexual intercourse, direct contact with lesions or perinatally. Most HSV positive cases are characterised by lesions on the skins and mucous membranes of the mouth and genitals. HSV infection can be either primary or a recurrence of a previous infection. More than 90% of the primary HSV infections are asymptomatic. Primary infection with HSV-1 can lead to gingivostomatitis, eczema herpeticum, keratoconjunctivitis and encephalitis. The primary symptoms of a secondary infection are skin lesions in the nose, mouth and genital regions. The infection is contagious, mainly during an epidemic.
HSV-2 TaqMan PCR Kit, 100 reactions
HSV-2 TaqMan PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM32450 (100 preps) | Cat. TM32410 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| HSV-2 Primer & Probe Mix | 280 μL | 280 μL |
| HSV-2 Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
