Propargyl-PEG10-acid

Overview

Grey, low profile PCR plate with a rigid two-component hardshell design which eliminates warping and distortion during PCR testing.

• Grey
• Polycarbonate frame
• Low-profile PCR plate
• Rigid two-component design eliminates warping and distortion during PCR
• 96W Clear Thin Walled PP Tubes
• Full Skirt
• Packaged 10 plates per sleeve, 50 plates per box

Grey, low profile PCR plate with a rigid two-component hardshell design which eliminates warping and distortion during PCR testing.
Grey
Polycarbonate frame
Low-profile PCR plate
Rigid two-component design eliminates warping and distortion during PCR
96W Clear Thin Walled PP Tubes
Full Skirt
Packaged 10 plates per sleeve, 50 plates per box

Overview

• Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
• Simple and quick workflow: library could be prepared in less than 5 hours
• Component of Norgen’s metagenomics workflow
• A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide 

The 16S V3-V4 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V3-V4 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The 16S V3-V4 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V3-V4 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.

Details

Storage Conditions and Product Stability
Norgen’s 16S V3-V4 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

Propargyl-PEG8-NHS ester is an amine reactive compound with an alkyne group. The alkyne group reacts with azide-containing biomolecules in Click Chemistry reactions, copper is needed as a catalyst. The hydrophilic PEG units helps the compound to have better solubility in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG8-NHS ester is an amine reactive compound with an alkyne group. The alkyne group reacts with azide-containing biomolecules in Click Chemistry reactions, copper is needed as a catalyst. The hydrophilic PEG units helps the compound to have better solubility in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.