Propargyl-PEG10-acid

Description

Attogene’s Mercury Lateral Flow Kit can be used for the screening of Mercury in water and food (fish) samples at 10 ppb in a laboratory setting.  Unlike the field-based kit, the lab kit is intended for a more technical end user who will be evaluating samples in a laboratory setting.  The instructions include method for extraction from food samples.

Mercury contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Mercury ion accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases.   Long-term health consequences of drinking Mercury-contaminated food and water include brain, heart, kidney, lungs  and immune system problems for adults, and the physical and mental development delays in infants and children.  Attogene’s Mercury Lateral Flow test gives results conforming of 10ppb or greater.  Using the supplied pipette, simply fill the vial with your water sample, place the water into the sample port and wait 5 minutes.

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Applications for Fish and Shellfish possible:

Mercury Levels in Commercial Fish and Shellfish (1990-2012)

Fish with recognized high concentrations of Mercury:

• Tuna, Grouper, Mackerel, Weakfish, Bluefish, Sablefish, Halibut, Bass Chilean, Shark, Marlin, Tilefish,

Screening of Mercury in water samples at 10 ppb
Format: 10 tests (5 tests/5 controls)
Run Time: 5 Minutes

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
• Higher intensity reference bands at 500 bp and 1000 bp
• 2686 bp (pUC19) reference band for easy clone identification

The CloneSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our CloneSizer contains fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments, double intensity reference bands at 500 and 1000 bp and an additional 2686 bp (pUC19) reference band for easy clone identification.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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CloneSizer 100 bp DNA Ladder (Cat# 11600) – 100 loads

Ladder Properties:

• Fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
• Higher intensity reference bands at 500 bp and 1000 bp
• 2686 bp (pUC19) reference band for easy clone identification

Fragment	Size (bp)	Mass (ng)
1	2686	72
2	2000	53
3	1500	41
4	1200	42
5	1000	56
6	900	30
7	800	29
8	700	25
9	600	25
10	500	52
11	400	19
12	300	20
13	200	19
14	100	17

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.