Propargyl-PEG10-NHS ester serves as a bifunctional PEG linker with a terminal propargyl that participates in click reactions with azide-bearing moieties and an NHS ester group that reacts readily and efficiently with amine-bearing molecules. The PEG10 units improve the water solubility of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Propargyl-PEG10-NHS ester serves as a bifunctional PEG linker with a terminal propargyl that participates in click reactions with azide-bearing moieties and an NHS ester group that reacts readily and efficiently with amine-bearing molecules. The PEG10 units improve the water solubility of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
HAM008 Yeast Extract Powder Technical
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Product Info
Introduction
Usages: Yeast extract promotes growth of a wide variety of microorganisms, including yeasts and moulds.
Technical specification: Total nitrogen(TN)—————————≥10.0% Amino nitrogen(AN) ————————≥4.5% Sulphuric ash———————————-≤15.0% Loss on drying——————————–≤6.0% Chlorides————————————–≤0.5% PH(5% solution)——————————7.0±0.5 Proteoses—————————————positive Tryptophan———————————— positive Nitrites—————————————–negative
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.
Fentanyl (FYL) Detection Kit (Rapid Lab) is a lateral flow chromatographic immunoassay for the qualitative detection of Fentanyl/Norfentanyl in liquid and powder substances at the cut-off concentration of 200 ng/mL.
It is specific for Fentanyl screening with no significant cross reactivity to other opiates, such as Morphine and Heroin.
Interpretation of test results are: positive (one line), negative (two lines), invalid (no lines or no control line).
• Sample: Liquid / Powder
• Format: Cassette
• Sensitivity: 200 ng/mL
• Specificity: Fentanyl
• Accuracy: > 98%
• Time-to-Result: 10 minutes
• Storage Condition: 2-30°C/36-86°F
• Test Principle: Lateral Flow Immunoassay
For professional institutions use only, not for self-testing.
D3182C HiPure Circulating DNA Midi Kit C (Vaccum Protocol)
Product Info
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Product Info
Introduction
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine.
This product is for research use only.
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Details
Specifications
Features
Specifications
Main Functions
Isolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol
Applications
qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (vacuum)
Sample type
Serum, plasma and other cell-free fluid samples
Sample amount
1-5ml
Elution volume
≥50μl
Time per run
≤60 minutes
Liquid carrying volume per column
4ml
Binding yield of column
1mg
Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer.
Advantages
High yield – most optimal process, free DNA (>50bp) can be obtained to the maximum extent
High concentration – low elution volume, ensuring high nucleic acid concentration
High purity – low alcohol binding method, completely removing inhibitor and protein pollution
High recovery – DNA can be recovered at thelevel of PG by silica gel column purification
Kit Contents
Contents
D318201C
D318202C
Purification Times
10
50
Buffer ACL
50 ml
250 ml
Buffer ACB*
60 ml
300 ml
Buffer DCW1*
4.4 ml
22 ml
Buffer DCW2*
5 ml
10 ml
Proteinase K
110 ml
540 mg
Protease Dissolve Buffer
10 ml
30 ml
Carrier RNA
110 μg
110 μg
Nuclease Free Water
10 ml
20 ml
HiPure CFDNA Mini Columns
10
50
2 ml Collection Tubes
20
100
Extender Tube
10
50
Vac-Connector
10
50
Storage and Stability
Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
Document
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments,