Propargyl-PEG10-NHS ester

Overview

• Free of all DNases, RNases and nucleic acids
• Prepared without the use of DEPC
• Room temperature storage

Norgen’s Nuclease-Free Water is free of all DNases, RNases and nucleic acids and is suitable for all molecular biology applications requiring nuclease-free water including PCR, RT-PCR and real-time PCR.  Our water is prepared without the use of DEPC, and is ready to use without further treatment.

Available Sizes

• 4 x 1.25 mL
• 100 x 1.25 mL
• 100 mL
• 500 mL
• 1000 mL

Details

Applications
For use in any molecular biology application

Storage Conditions
Norgen’s Nuclease-Free Water should be stored at room temperature. Storage at +4°C or -20°C is optional.

Precautions and Disclaimers
This product is designed for research purposes only.  It is not intended for human or diagnostic use.

Description 

YesBlot™ Western Marker I is a ready-to-use mixture with ten IgG-binding proteins covering a wide range of molecular weights from 15 to 200 kDa in Tris-Glycine buffer. YesBlot™ Western Marker I performs dual functions. First, it contains 4 pre-stained proteins (10, 25, 45 and 70 kDa) for monitoring protein separation during SDS-PAGE, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the protein size. Second, ten IgG-binding proteins can be immuno-detected on film or by CCD imaging. YesBlot™ Western Marker I is compatible for chemiluminescent, fluorescent, chromogenic or other detection systems. In addition, YesBlot™ Western Marker I has two reference bands with enhanced intensity (at 30 kDa and 80 kDa). The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading. 

Features

• Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots
• Pre-stained bands — 4 prestained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
• Wide range — 10 clear bands from 15 to 200 kDa for size estimation
• Quick reference — two enhanced bands (30 and 80 kDa)

Contents 

The YesBlot™ Western Marker I contains recombinant IgG binding proteins, prestained recombinant proteins, glycerol and SDS. 

Storage

4°C for 3 months
-20°C for 24 months

YesBlot™ Western Marker I is a ready-to-use mixture with ten IgG-binding proteins covering a wide range of molecular weights from 15 to 200 kDa in Tris-Glycine buffer. YesBlot™ Western Marker I performs dual functions. First, it contains 4 pre-stained proteins (10, 25, 45 and 70 kDa) for monitoring protein separation during SDS-PAGE, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the protein size. Second, ten IgG-binding proteins can be immuno-detected on film or by CCD imaging. YesBlot™ Western Marker I is compatible for chemiluminescent, fluorescent, chromogenic or other detection systems. In addition, YesBlot™ Western Marker I has two reference bands with enhanced intensity (at 30 kDa and 80 kDa). The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.

LyoCakes are ready-to-use, freeze-dried master mixes pre-aliquoted PCR-strips or reaction-tubes.

LyoCakes contain: DNA polymerase(s), reaction buffer, dNTPs and primers and probes. They are rehydrated within seconds in any aqueous solutions, which  makes reaction setup very easy. Simply add a biological sample and place the tube in a PCR cycler. 

Storage: LyoCakes are shipped and stored simply at room-temperature. This provides a more cost efficient and ecological distribution compared to other master mixes.

As this is a customized product, we take your requirements very serious. You would like to know more? Please get in touch! 

LyoCakes are ready-to-use, freeze-dried master mixes pre-aliquoted PCR-strips or reaction-tubes.

LyoCakes contain: DNA polymerase(s), reaction buffer, dNTPs and primers and probes. They are rehydrated within seconds in any aqueous solutions, which makes reaction setup very easy. Simply add a biological sample and place the tube in a PCR cycler.