Propargyl-PEG12-methylamine is a propargyl linker that is commonly used as a Click Chemistry reagent for copper-catalyzed reactions with azides. The methylamine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The PEG spacer helps improve the water solubility of the molecule in aqueous media. Reagent grade, for research use only.
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Propargyl-PEG12-methylamine is a propargyl linker that is commonly used as a Click Chemistry reagent for copper-catalyzed reactions with azides. The methylamine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The PEG spacer helps improve the water solubility of the molecule in aqueous media. Reagent grade, for research use only.
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Propargyl-PEG7-acid
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Propargyl-PEG7-acid is a reagent with a propargyl group with a carboxylic acid. The carboxylic acid reacts with primary amines under the activation of EDC or HATU. The propargyl group can participate in azide-alkyne Click Chemistry reaction to form triazole linkage, copper is required as a catalyst. The PEG spacer increases the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG7-acid is a reagent with a propargyl group with a carboxylic acid. The carboxylic acid reacts with primary amines under the activation of EDC or HATU. The propargyl group can participate in azide-alkyne Click Chemistry reaction to form triazole linkage, copper is required as a catalyst. The PEG spacer increases the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Purify sequencing extension products from dye terminators, primers and other contaminants
Also purify DNA from different enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations.
High recovery
Fast and efficient spin column format
Also available in 96 well format for high throughput
This kit provides a rapid spin column procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on spin-column chromatography without the use of phenol, chloroform or alcohol precipitation. The kit provides a high quality product with up to 90% recovery.
The kit is also available in a 96-well format for high-throughput sequencing reaction clean-up. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Internal Lane Standard (60bp – 600bp, ROX) for ABI Genetic Analyzer
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Ready-to-use
Highly stable
Precise
22 discrete bands from 60 bp to 600 bp
High quality ready-to-use double-stranded DNA ladder derived by recombinant technology for precise size and mass determination in various fluorescence-detection instruments including different models of Applied Biosystems® PRISM® and Genetic Analyzers
22 discrete bands, ranging from 60 bp to 600 bp
The ladder is asymmetrically labelled with ROX that can be detected by excitation at 576 nm and fluorescent emission at 597 nm
Compatible with products for fragment analysis including Promega PowerPlex® 16 System