Propargyl-PEG13-bromide is a heterobifunctional reagent that can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The bromide (Br) can be used in nucleophilic substitution reactions. The PEG units increase water-solubility of the molecule in in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Propargyl-PEG13-bromide is a heterobifunctional reagent that can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The bromide (Br) can be used in nucleophilic substitution reactions. The PEG units increase water-solubility of the molecule in in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Efficient depletion of bovine exosomes from Fetal Bovine Serum
Deplete exosome-sized vesicles from versatile FBS volumes
No protease treatment required
No time-consuming ultracentrifugation
No precipitation reagents required
No overnight incubation required
Exosome depletion confirmed by reduction of bovine miRNAs below detectable levels
The depleted FBS provides the same cellular growth rates as the standard FBS
Purification is based on Norgen’s proprietary Silicon Carbide resin matrix
Norgen’s FBS Exosome Depletion Kits provides a quick and easy protocol for the depletion of bovine exosomes from FBS prior to using it as a growth supplement in your culture medium. The FBS recovered from the depletion process is exosome-depleted and does not contain any quantifiable bovine miRNAs. Moreover, the exosome-depleted FBS will support the growth of your cells of interest similar to the non-depleted FBS. Norgen’s kits allow for the processing of different FBS volumes. The depletion is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require ultracentrifugation, any special instrumentation, precipitation reagents or any protease treatments. More importantly, the depletion process is an inexpensive method for exosome depletion from FBS, as compared to the expensive current ready-to-use exosome-depleted media available on the market.
Background
Most culture medium used for the growth and propagation of cells in culture require the addition of fetal bovine serum (FBS) as a growth supplement to media. FBS is obtained from bovine (cow) serum, and therefore contains large quantities of bovine exosome vesicles. These exosomes may interfere with some types of studies, or may lead to unreliable results when studying the exosomes shed from your cells of interest in normal culture conditions. Therefore, the use of exosome-depleted FBS is highly recommended for many types of studies.
Up to 140 mL (FBS Exosome Depletion Kit I (Slurry Format) Up to 280 mL (FBS Exosome Depletion Kit II (Slurry Format)
Depletion
Deplete exosome-sized vesicle
Bovine miRNA
No detectable bovine miRNA
Time to Complete 6 Purifications
40 minutes
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
AccuBand™ 50 bp DNA Ladder II is composed of 13 individual DNA fragments, presenting 1k, 900, 800, 700, 600, 500, 400, 300, 250, 200, 150, 100 and 50 bp sharp bands respectively. This product contains 2 enhanced bands (500 bp and 250 bp) for easy band identification. AccuBand™ 50 bp DNA Ladder II is ready-to-use, containing loading buffer with a tracking dye (orange G). AccuBand™ 50 bp DNA Ladder II provides a sufficient amount of DNA for clear observation of all DNA bands ranging from 50 bp to 1 kb, either in agarose gel or in polyacrylamide gel electrophoresis.
Features
Sharp bands
Suitable for polyacrylamide gel electrophoresis
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
50 ~ 1,000 bp
Concentration
55.5 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months 4°C for 12 months -20°C for 36 months
Document
AccuBand™ 50 bp DNA Ladder II is composed of 13 individual DNA fragments, presenting 1k, 900, 800, 700, 600, 500, 400, 300, 250, 200, 150, 100 and 50 bp sharp bands respectively. This product contains 2 enhanced bands (500 bp and 250 bp) for easy band identification. AccuBand™ 50 bp DNA Ladder II is ready-to-use, containing loading buffer with a tracking dye (orange G). AccuBand™ 50 bp DNA Ladder II provides a sufficient amount of DNA for clear observation of all DNA bands ranging from 50 bp to 1 kb, either in agarose gel or in polyacrylamide gel electrophoresis.