Description

About the genesig q32 Real-Time PCR Instrument

genesig 32 Overview
The genesig q32 Real-Time PCR Instrument is one of the fastest qPCR instruments on the market today due to its rapid heating and cooling. Assembled from just a few building blocks, this robust qPCR instrument allows analysis of up to 32 samples in both tube or strip format. The powerful genesig q32 software is easily installed from a USB flash drive onto any PC or Mac. The genesig q32 is used with 550 genesig Real-Time PCR Kits for human, vet, and food pathogens, as well as food speciation testing

The genesig Real-Time PCR Kit product range includes tests for a massive range of applications:

• Human Infectious Disease Screening
• Veterinary Diagnostics
• Food and Water testing
• Biothreat Detection

genesig q32 – Cutting edge design and technology
Assembled from just a few building blocks the robust genesig q32 system is easy to transport and install. Up to 32 samples can be run in 0.1 ml tubes or 8-strip format. Fast heating and cooling is achieved by utilizing robust Peltier elements, whilst assay performance is supported further by a heated lid design. Excitation is provided by high intensity LEDs filtered to provide light at 500nm that is capable of exciting all fluorophores commonly used in qPCR. A prism is used to generate spectra from fluorescent emissions. These spectra are imaged using a CMOS camera.

genesig q32 software
The genesig q32 software is delivered and easily installed from a USB flash drive. When installed in a network, the software can control several q32 instruments connected via LAN. The powerful and easy to use software provides the following features:

• Quick start using templates for all genesig kit applications
• Straightforward setup and editing of sample and target information
• Automated analysis modules for absolute and relative quantification, melting curve analysis and endpoint genotyping
• Analysis of full spectral data
• Compatible with Windows, Mac & Linux Systems
• Instrument start from a USB flash drive, using preprogrammed settings
• Data export functions

genesig Real-Time PCR Kit compatible
genesig Real-Time PCR Kits are suitable for use on the genesig q32 instrument. The genesig Kit range includes over 550 kits for human, vet, and food pathogens, as well as food speciation testing. All genesig Kits include a positive control and resuspension buffers. The kits come freeze-dried so that they can be shipped at room temperature for fast delivery to anywhere in the World.

Automated run templates available for:

• genesig Easy Real-Time PCR Kits
• genesig Advanced Real-Time PCR Kits
• genesig Standard Real-Time PCR Kits
• snpsig Real-Time PCR Kits
• quasa Real-Time PCR Kits
• GMO Real-Time PCR Kits

Dimensions: W 24 cm x D 27 cm x H 23 cm
Weight: 7 kg
Operating noise: Electrical: Voltage: 100 – 240 V ( 10%); Frequency: 50 – 60 Hz ( 10%); Power: 170 W
Number of reactions: 32
Reaction vessels: 0.1ml tube and/or 8-tube strips
Reaction volume: 10 – 100 μl (20 μl recommended)
Temperature cycling system: Peltier-based heating & cooling from 40 to 99°C; Heating rate: 4°C/s & Cooling rate: 3°C/s
Temperature uniformity:  /- 0.05°C (SD)
Temperature accuracy:  /- 0.25°C
Run time: Fluorescence excitation: 500 nm nm (blue LED)
Fluorescence detection: 120 optical channels from 510 to 750 nm (CMOS camera)
Factory-calibrated dyes (at shipment): FAM; VIC / HEX
Applications: Relative and absolute quantification analysis; Melting peak analysis; Endpoint genotyping analysis
Sensitivity: 1.1 fold discrimination; 9-log dynamic range; Single copy detection
Connection options: LAN; Direct connection to computer (RJ45); PC-free (USB stick/flash drive controlled)Candidatus Liberibacter asiaticus Manual

Guaranteed results using 550 genesig kits
Fast DNA and RNA analysis in less than 60 minutes
Quick and easy set-up and run
Automated data analysis result calling
Small and robust instrument
Exceptional value for money

N-(Acid-PEG2)-N-bis(PEG2-propargyl) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

N-(Acid-PEG2)-N-bis(PEG2-propargyl) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

HiPure RNA Clean Up Kit provides a rapid and easy method for the purification and concentrate RNA from enzymatic reactions or for desalting the RNA samples. RNA purified using HiPure RNA Clean Up Kit is ready for all downstream applications such as RT-PCR, Northern blotting, mRNA purification, nuclease protection, and in vitro translation.

Details

Specifications

Features	Specifications
Main Functions	Purification and concentration of RNA（mRNA>200nt or miRNA）from transcription products, DNase cleavage products, labeled products, and crude RNA products
Applications	Sequencing, ligation, enzyme digestion, RT-PCR, labeling, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Crude RNA, enzymatic reaction solution
Sample amount	≤100μl
Recovery	90%
Elution volume	≥15μl
Time per run	≤15 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Binding buffer is added directly to the sample or other enzymatic reaction, and the mixture is applied to the column. Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure RNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.

Advantages

• High recovery – recovery of RNA up to 90%
• Low elution volume – the elution volume can be as low as 15µl
• Fast – only 10 minutes for recovery by using column method
• General – suitable for various crude RNA products
• Wide range of fragment recovery:(>25nt RNA)

Kit Contents

Contents	R214402	R214403
Purification Times	50 Preps	250 Preps
Buffer GXP	30 ml	120 ml
Buffer RW2	20 ml	2 x 50 ml
RNase-Free Water	20 ml	60 ml
HiPure RNA Mini Columns I	50	250
2 ml Collection Tubes	50	250

Storage and Stability

The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

HiPure RNA Clean Up Kit provides a rapid and easy method for the purification and concentrate RNA from enzymatic reactions or for desalting the RNA samples. RNA purified using HiPure RNA Clean Up Kit is ready for all downstream applications such as RT-PCR, Northern blotting, mRNA purification, nuclease protection, and in vitro translation.