Propargyl-PEG14-t-butyl ester consists of a propargyl group and a t-butyl protected carboxyl group. The propargyl group can be used in copper catalyzed Click Chemistry to yield a stable triazole linkage with azides. The t-butyl group can be hydrolyzed in acidic conditions. The hydrophilic PEG units help the molecule to have better solubility in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG14-t-butyl ester consists of a propargyl group and a t-butyl protected carboxyl group. The propargyl group can be used in copper catalyzed Click Chemistry to yield a stable triazole linkage with azides. The t-butyl group can be hydrolyzed in acidic conditions. The hydrophilic PEG units help the molecule to have better solubility in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
T7 RNA Polymerase catalyzes the formation of RNA from a DNA template in the 5’-3’ direction and is commonly used in in vitro transcription (IVT) applications. The enzyme is T7 promoter specific, requires Mg2+ as a cofactor and can use modified nucleotides for the synthesis. The T7 RNA Polymerase requires a double-stranded DNA template and can produce full-length RNA transcripts.
Key Features
Suggested Applications
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Quality Control
ArcticZymes is dedicated to the quality of our products. T7 RNA Polymerase is manufactured at our ISO 13485 certified facility in Norway.
T7 RNA Polymerase catalyzes the formation of RNA from a DNA template in the 5’-3’ direction and is commonly used in in vitro transcription (IVT) applications. The enzyme is T7 promoter specific, requires Mg2+ as a cofactor and can use modified nucleotides for the synthesis. The T7 RNA Polymerase requires a double-stranded DNA template and can produce full-length RNA transcripts.
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 250-500mg soil sample |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Soil |
| Sample amount | 250-500mg |
| Elution volume | ≥50μl |
| Time per run | ≤70 minutes |
This product is based on the purification method of high binding magnetic particles. Soil sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our propietary Absorber Solution. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
| Contents | D635601 | D635602 | D635603 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagPure Particles | 2.5 ml | 5 ml | 22 ml |
| 2ml Bead Tubes | 48 | 96 | 400 |
| Buffer SOL | 60 ml | 100 ml | 500 ml |
| Buffer SDS | 6 ml | 10 ml | 50 ml |
| Reagent DX | 1 ml | 1 ml | 5 ml |
| Buffer PS | 10 ml | 20 ml | 90 ml |
| Absorber Solution | 10 ml | 20 ml | 90 ml |
| Buffer GDP | 70 ml | 150 ml | 2 x 350 ml |
| Buffer GW1* | 22 ml | 44 ml | 220 ml |
| Elution Buffer | 20 ml | 20 ml | 60 ml |
Storage and Stability
MagPure Particles and Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free cf-DNA/ct-DNA/cf-RNA in a very small elution volume.
These tubes are ideal for collecting and preserving cf-DNA and cf-RNA samples for in vitro diagnostic use for medical purposes.
Performance
Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx abridge the collection/processing of whole blood for the subsequent purification of cf-DNA and/or cf-RNA. The cf-DNA/cf-RNA Preservative Tubes Dx preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA/RNA into plasma. Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma. The cf/ct DNA and cf-RNA levels are stable for up to 30 days at room temperature. The cf-DNA is also stable for up to 8 days at 37°C.
Workflow
NOTE: This product is not available for sale in the United States. For the research use only version, see Cat. 63950
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| Kit Specifications | |
| Tube Size | 16 x 100 mm |
| Tube Type | PET Tube |
| Blood Draw Volume | 8.4 mL in 10 mL Tubes |
| Preservative Volume | 1.6 mL |
| Recovered Plasma Volume | Approximately 6-7 mL (not affected by shipping) |
| Anticoagulant | Proprietary |
| Preservative | Non formaldehyde – Proprietary preservation agent |
| Length of cf-DNA Preservation | Up to 30 days at room temperature (15-25°C) Up to 8 days at 37°C |
| Length of cf-RNA Preservation | Up to 30 days at room temperature (15-25°C) |
| Length of CTCs Preservation | Up to 14 days at room temperature (15-25°C) |
Storage Conditions and Product Stability
| Component | Cat. Dx63950 (50 tubes) |
|---|---|
| cf-DNA/cf-RNA Preservative Tubes Dx | 50 |
| Product Insert | 1 |
| Flowchart | 1 |
