Propargyl-PEG2-alcohol

Description

The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 10 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors. 

Features

• Ideal for PCR amplification and cDNA synthesis
• Premixed solution
• Nuclease and ribonuclease free

Applications 

• PCR amplification of DNA fragments
• DNA fill-in reaction
• DNA sequencing
• Reverse transcription
• One-step RT-PCR

Storage

-20°C for 36 months

The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 10 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors.

Character

Yingtai   pilot-type   freeze   dryer   completely   simulates   the   technical   requirements   of  the   industrial   freeze   dryer,with   a   compact   and   reasonable design,a   large   redundancy   range,and   conforms   to   GMP  and   FDA   standards.The   ice   condensation   capacity   is   4~18kg,and  the  WRL  controller and   liquid   temperature   control   shelf   are   adopted,so   that   the    process   of   pre-freezing   and   freeze-drying   can    be   precisely   controlled   on   the main   machine,and   the    process   results    are   quite   comparable   with   the    production   machine.In    addition   to    supporting   the   eutectic    point   test system,pressure    rise    and     pressure    comparison     method    to    determine     the    freeze-drying    end     point    and     freeze-drying    curve    recording software,hallan   pilot-type   freeze    dryer   can   also    be   equipped   with    engineering   WRL   control   software   to   directly    Graphical   computer   control the   freeze-drying   process.It    can   be    configured   with    H202 sterilization    and   high-pressure    steam   sterilization    orintegrated   glove    box   system, which can be used for clean room operation through the wall.The cold trap and freeze-drying chamber are all designed with 316L stainless steel  to  ensure  the  cleanliness  of  the  chamber  and  good   ultimate  vacuum  and  vacuum   leakage  rate  to  the  greatest  extent.

Refrigeration Series

●  Dual  compressors,Secop  special   compressors,air  cooling (water  cooling  system  is  optional)

●   Refrigeration   method:Cascade   refrigeration    method,high   cooling efficiency,good   reliability,using   CFC-Free   environmentally   friendly refrigerant.

Silicone oil conducts heat,with uniform temperature and accuracy up to  0.1°C.Silicone oil has a wide temperature range,up to-100°C~+600°C.

● Shelf refrigeration system and cold trap refrigeration system are completely independent,without any switching,refrigeration temperature is lower and more stable.

Vacuum   series

●      Vacuum      pump:German       Pfeiffer      anti-corrosion,anti-oil      return       function vacuum   pump.(Vacuum    pump   can    be   selected    according   to    user   needs)

●       Pumping        rate:9m³/h~62m³/h,multiple        specifications       are        optional,the ultimate  vacuum  can  reach  2×10-3mbar,and  the  oil  mist  filter  is  standard.

●   The   vacuum    pipelines    are   all    high-vacuum   stainless    steel   vacuum    tubes, which  can  effectively  prevent  vacuum  leakage  and  corrosion.

●   The    double   anti-oil    return    device    completely   eliminates    the    damage   and

equipment   and   samples.

●    Automatic    backfill    air    device,the    system     has    a    built-in     High    efficiency particulate  air(HEPA)filter  to  ensure  the  complete  cleanliness  of  backfill  air.

●   The   vacuum   stabilization   system    can   stabilize   the   vacuum    degree   of   the freeze-drying    process    through     the    micro-pass    vacuum    control    valve    to ensure  that  the  samples  will   not  be  affected  by  excessive  fluctuations   in  the vacuum    degree.

●   Condensed    liquid   collection   and   discharge   system,automatically   completes the   collection   and   discharge   of   condensed   liquid   after   freeze-drying.

Control    System Characteristic

●               Control:Automatic/Manual

●   Can   store    at   least    100    independent   freeze-drying    programs,each   program    contains   32    program   segments    (Can   be

customized   according    to    user    needs),each   program   segment    corresponds    to   a    processing    stage    (pre-freezing,main

drying,post-drying).and    Include     the    following     segment    values:Cycles,shelf     temperature,vacuum    and     safety    pressure

●  All  the  equipment  can   be   recorded   in   real   time   and   the   corresponding   freeze-drying   curves   and   data  tables   can   be generated  after  the   end.The  records  of  all   data  and  curves  comply  with   the  corresponding  regulations  and  standards.

●    It    has    hierarchical    management    authority,hierarchical    management    allocation    authority    and    usage    process    tracking authority

● Remote    System

O   Fully   support   2G/4G/GSM/Wi-Fivarious   connection    modes

o  Users can remotely view all data on the device and operate it

o  Technicians  can  remotely  perform  maintenance  and  fault  detection  on  equipment,and  can  remotely  guide  and  train  users

o  Can  remotely  upgrade  and  change  the  system  of  the  equipment

Parameter

Yingtai pilot-type freeze dryer completely simulates the technical requirements of the industrial freeze dryer,with a compact and reasonable design,a large redundancy range,and conforms to GMP and FDA standards.The ice condensation capacity is 4~18kg,and the WRL controller and liquid temperature control shelf are adopted,so that the process of pre-freezing and freeze-drying can be precisely controlled on the main machine,and the process results are quite comparable with the production machine.In addition to supporting the eutectic point test system,pressure rise and pressure comparison method to determine the freeze-drying end point and freeze-drying curve recording software,hallan pilot-type freeze dryer can also be equipped with engineering WRL control software to directly Graphical computer control the freeze-drying process.It can be configured with H202 sterilization and high-pressure steam sterilization orintegrated glove box system, which can be used for clean room operation through the wall.The cold trap and freeze-drying chamber are all designed with 316L stainless steel to ensure the cleanliness of the chamber and good ultimate vacuum and vacuum leakage rate to the greatest extent.

Overview

• Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
• Simple and quick workflow: library could be prepared in less than 5 hours
• Component of Norgen’s metagenomics workflow
• A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide 

The 16S V1-V3 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The 16S V1-V3 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V3 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.

Details

Supporting Data

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Storage Conditions and Product Stability
Norgen’s 16S V1-V3 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.