Propargyl-PEG2-methylamine is a crosslinker with a propargyl group and methylamine group. The propargyl group forms triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The methylamine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG2-methylamine is a crosslinker with a propargyl group and methylamine group. The propargyl group forms triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The methylamine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Norgen’s Streptococcus agalactiae TaqMan PCR Lyophilized Kit is designed for the detection of Streptococcus agalactiae specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
Norgen’s Streptococcus agalactiae TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of Streptococcus agalactiae specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
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| Component | Cat. TM30650L (100 preps) | Cat. TM30610L (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix (Lyo) | 4 x 350 μL | – |
| Streptococcus agalactiae Primer & Probe Mix (Lyo) | 280 μL | 280 μL |
| Streptococcus agalactiae Positive Control (Lyo) | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 3 x 1.25 mL | 1 x 1.25 mL |
| Product Insert | 1 | 1 |
Norgen’s FBS Exosome Depletion Kits provides a quick and easy protocol for the depletion of bovine exosomes from FBS prior to using it as a growth supplement in your culture medium. The FBS recovered from the depletion process is exosome-depleted and does not contain any quantifiable bovine miRNAs. Moreover, the exosome-depleted FBS will support the growth of your cells of interest similar to the non-depleted FBS. Norgen’s kits allow for the processing of different FBS volumes. The depletion is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require ultracentrifugation, any special instrumentation, precipitation reagents or any protease treatments. More importantly, the depletion process is an inexpensive method for exosome depletion from FBS, as compared to the expensive current ready-to-use exosome-depleted media available on the market.
Background
Most culture medium used for the growth and propagation of cells in culture require the addition of fetal bovine serum (FBS) as a growth supplement to media. FBS is obtained from bovine (cow) serum, and therefore contains large quantities of bovine exosome vesicles. These exosomes may interfere with some types of studies, or may lead to unreliable results when studying the exosomes shed from your cells of interest in normal culture conditions. Therefore, the use of exosome-depleted FBS is highly recommended for many types of studies.
FBS Exosome Depletion Kit (Slurry Format)
For FBS volumes ranging from 140 mL to 280 mL.
FBS Exosome Depletion Kit (Column Format)
For FBS volumes ranging from 120 mL to 240 mL.
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| Kit Specifications | |
| Sample Type | Fetal Bovine Serum |
| Sample Volume Range | Up to 140 mL (FBS Exosome Depletion Kit I (Slurry Format) Up to 280 mL (FBS Exosome Depletion Kit II (Slurry Format) |
| Depletion | Deplete exosome-sized vesicle |
| Bovine miRNA | No detectable bovine miRNA |
| Time to Complete 6 Purifications | 40 minutes |
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 61100 (6 preps) | Cat. 61400 (12 preps) | Cat. 61200 (6 preps) | Cat. 61300 (12 preps) |
|---|---|---|---|---|
| ExoC Buffer | 2 x 1.5 mL | 8 mL | 2 x 1.5 mL | 8 mL |
| Slurry E | 12.5 mL | 2 x 12.5 mL | – | – |
| Maxi Spin Column | – | – | 6 | 12 |
| Product Insert | 1 | 1 | 1 | 1 |
Thermal adapter for PCR plates, e.g., NEST 96 Well Plate 100 µL PCR Full Skirt. Compatible with the Opentrons Heater-Shaker Module.
Thermal adapter for PCR plates, e.g., NEST 96 Well Plate 100 µL PCR Full Skirt. Compatible with the Opentrons Heater-Shaker Module.
