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Propargyl-PEG2-t-butyl ester

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Propargyl-PEG2-t-butyl ester is a PEG linker that enables Click Chemistry reactions with azide-bearing compounds or biomolecules; copper will be required for catalyzation. Under acidic conditions, the t-butyl group can be removed. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Detail

Propargyl-PEG2-t-butyl ester is a PEG linker that enables Click Chemistry reactions with azide-bearing compounds or biomolecules; copper will be required for catalyzation. Under acidic conditions, the t-butyl group can be removed. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Other Products

ProteoSpin™ Urine Protein Concentration Kits

Overview

  • Simultaneous concentration, desalting and buffer exchange of total urinary proteins
  • No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
  • Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

These kits concentrate urine proteins while simultaneously removing salts, urea, and other urine contaminants. There is no molecular weight cut-off and therefore the columns capture total urinary proteins and peptides of all sizes making them ideal for biomarker discovery work, differential expression of proteins in various diseases, or other diagnostic research. The columns are convenient, rapid and easy to use and thus offer significant time savings over classic dialysis protocols. The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the sample conveniently for downstream proteomic applications including SDS-PAGE, 2D Gels, MALDI-TOF, LC/MS, LC/MS/MS, whole protein mass spectrometry, western blotting, protein microarrays, and more.

ProteoSpin™ Urine Protein Concentration Micro Kit

Each spin column is able to concentrate and desalt up to 200 μg of urine proteins. Twelve samples can be processed in 20 minutes.

ProteoSpin™ Urine Protein Concentration Midi Kit

The ProteoSpin™ Urine Protein Concentration Midi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 1 to 5 mL inputs of urine. Each mini spin column is able to concentrate and desalt up to 3 mg of urine proteins in 30 minutes.

ProteoSpin™ Urine Protein Concentration Maxi Kit

The ProteoSpin™ Urine Protein Concentration Maxi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 2 to 20 mL inputs of urine. Each maxi spin column is able to concentrate and desalt up to 4 mg of urine proteins in 45 minutes.

Details

Supporting Data

Figure 1 / 7

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Figure 1.  Two-Dimensional (2D) Gel Analysis
Figure 1.  Two-Dimensional (2D) Gel Analysis
Figure 2. Consistent Protein Purification from 1 mL Urine Samples
Figure 3. Western Blot Showing the Recovery of Proteins Spiked into Urine
Figure 4. High Yield of Urinary Proteins
Figure 5. Purification of Total Proteins
Figure 6. No Loss of Urine Proteins
Figure 7. Concentration of Urine Proteins

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Kit Specifications
Maximum Urine Input Volume20 mL
Maximum Recovered Protein4 mg
Minimum Elution Volume2 mL
Time to Process 4 Samples45 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

ComponentCat. 17400 (25 preps)Cat. 52300 (10 preps)Cat. 21600 (4 preps)
Wash Solution C60 mL60 mL130 mL
Binding Buffer A4 mL4 mL8 mL
Elution Buffer C8 mL30 mL30 mL
Protein Neutralizer4 mL4 mL4 mL
Micro Spin Columns25
Midi Spin Columns (assembled with collection tubes)10
Maxi Spin Columns (assembled with collection tubes)4
Collection Tubes25
Elution Tubes (1.7 mL)25
Midi Elution Tubes10
Elution Tubes (50 mL)4
Product Insert11

CO00018 Midstream Cassette 140x15mm, 86x6mm pink w/lens

Description

  • Appearance: White/Pink with lens
  • • Length of strip: 86mm
  • • Width of strip:  6mm
  • • Length of device: 140mm
  • • Width of device: 15mm

NGS FFPE DNA Library Prep Kit (illumina and MGI Platforms)

The NGS FFPE DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality FFPE DNA libraries using 10 ng to 400 ng of input DNA isolated from formalin-fixed, paraffin-embedded (FFPE) samples. The DNA damage caused by fixation makes it difficult to construct high quality libraries. Our kit has been optimized to repair damaged DNA in the reactions. Multiplexing of the NGS FFPE DNA Library is possible.

NGS FFPE DNA Library Prep Kit Workflow

FFPE samples are a great resource for biomedical research. However, the methods for fixation and condition of storage significantly damage the DNA in the samples.  Thus, the extraction of high quality DNA from FFPE samples is often a challenge.  Low yield and low quality of FFPE DNA usually are common because of the limited tissue material and the DNA degradation.

As a result, it is usually difficult to construct high quality NGS libraries from low amount and low quality of FFPE DNA. In order to address this issue, we have developed the NGS FFPE DNA Library Prep Kit to make high quality libraries from the low input of FFPE DNA samples.

Three index types are available for the NGS FFPE DNA Library Prep Kit of the illumina platform:

Non-index (Cat.# 30035): Libraries do not have index.

Index (Cat.# 30037): Each of our index primers contains a unique barcode DNA (6 bases long) that can be used to identify individual library. Multiplexing of libraries is up to 48 samples. Index information can be downloaded here.

Unique dual index (Cat.# 30039): FFPE DNA library multiplexing is possible with 96 samples based on the unique dual indexing system. Our unique FourBase Difference Index System allows us to make indexes that have at least 4 bases different from each other in the 8-base index sequence. Our unique dual indexing primers can effectively remove NGS errors including index hopping, de-multiplexing errors, index cross-contamination, mis-assignments etc. The unique dual index primer set includes 96 pre-mixed unique pairs of i5 and i7 index primers. Index information can be downloaded here.

Indexes are available for the MGI platform kits (Cat.# 34037).

Kit advantages:

  • Fast and simple protocol
    • Making libraries in just 1.5 hours
    • 10 minutes of hands-on time
  • Easy procedure
    • Ready-to-use master mix to reduce the tedious mixing
    • Less reaction components to simplify the reaction setup
  • Less magnetic beads needed for the purification steps: saving more than 50% of the expensive beads
  • Low input FFPE DNA: From 10 ng to 400 ng

NGS FFPE DNA Library Prep Kit

Comparison of library conversion efficiency under the same condition. Input DNA amount is 25 ng.

NGS FFPE DNA Library Prep Kit

Comparison of library yield under the same condition. Input DNA amount is 25 ng.