Propargyl-PEG2-t-butyl ester

Description

Attogene Fluorescent Universal Lateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a fluorescent lateral flow dipstick assay for detection of DNA and RNA products.

Fluorescent lateral flow assays can be 2-100 fold more sensitive that gold based lateral flow tests.  Europium dyed polystyrene beads conjugated to streptavidin

Formats (fluorescent broad range UV light excitation range of 300nm to 400nm, 610nm emission) Streptavidin conjugate pad):

• • Detectable using a black light such as a black light UV flashlight or fluorescent lateral flow reader.
• • Detection of nucleic Acid (DNA or RNA) requires the use of a biotin and FAM-labelled primer during amplification. 
• • Test line: anti-FITC/FAM
• • Control Line: Biotin
• • Multiplex detection of nucleic Acid (DNA or RNA) requires the use of a biotin, FAM and Dig labelled primers during amplification.:
• • Test Line #1: anti FITC/FAM, Line #2: anti-Dig, Line #3 Biotin.

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Inquire about custom configurations: sales@attogene.com. 

For example, this product can be configured with alternative/custom streptavidin fluorescent particles.

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Kit Components 

• 50 -4.5mm Fluorescent Lateral Flow Dipsticks
• 10 mL Sample assay running buffer

Features & Benefits

• Can be used for development of a lateral flow assay for detection of a variety of different molecules such as amplified DNA products from PCR, LAMP and RPA reactions.
• No need to stripe capture antibodies
• No expensive equipment required (Black Light)
• Cost-effective way to screen for further downstream lateral flow assay development.

Description 

The DM5100 ExcelBand™ Super Range DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM5100 is composed of 26 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100 and 50 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains four enhanced bands (3k, 1.2k, 500 and 200 bp) for easier reference. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are added for real time monitoring.

Features

• Sharp bands
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA. 

Range 

50 ~ 25,000 bp 

Concentration 

88.7 µg/ 500 µl 

Recommended loading volume 

5 µl/ well 

Storage

Room temperature for 6 months
4°C for 12 months 
-20°C for 36 months

This test has been authorized by FDA under an EUA for use by authorized laboratories.

Description

The Primerdesign Ltd COVID-19 genesig® Real-Time PCR assay is a real-time RT-PCR assay intended for the qualitative detection of nucleic acid from SARS-CoV-2 in oropharyngeal swab specimens from patients suspected of COVID-19 by their healthcare provider. Testing is limited to laboratories certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA), 42 U.S.C. §263a, to perform high complexity tests.

*FDA clinical study. See Instructions for Use for additional clinical data.

FOR US ONLY
Rapid detection and exclusive to the COVID-19 strain
Does not detect other related coronavirus strains
High priming efficiency
Accurate controls to confirm extraction, and assay validity
Lyophilised components for ambient shipping
Highly specific detection profile
This test has been authorized by FDA under an EUA for use by authorized laboratories.