Propargyl-PEG3-acid is a reagent with an alkyne group and a carboxylic acid. The terminal carboxylic acid can form amide bond with primary amines (activation is needed). The alkyne can form triazole linkage with azide containing molecules via copper catalyzed reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG3-acid is a reagent with an alkyne group and a carboxylic acid. The terminal carboxylic acid can form amide bond with primary amines (activation is needed). The alkyne can form triazole linkage with azide containing molecules via copper catalyzed reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
These kits are designed to clean-up and concentrate RNA (including miRNA) from TRIzol® and TRI Reagent®, enzymatic reactions, in vitro transcription, labelling reactions, etc. These are robust kits for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications. These kits purify all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.
Norgen’s RNA Clean-Up and Concentration Kit provides a rapid method for the purification, cleanup and concentration of up to 35 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 20 µL. Complete 10 purifications in 20 minutes.
Norgen’s RNA Clean-Up and Concentration 96-Well Kit provides a rapid method for the purification, cleanup and concentration of up to 50 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 75 µL. Complete 10 purifications in 30 minutes.
Norgen’s RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. This kit is made for eluting RNA in smaller volumes of 8 µL for all types of downstream applications, for the highest concentration of RNA sample. Complete 10 purifications in 20 minutes.
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| Kit Specifications (Spin Column) | |
| Maximum Column Binding Capacity | 35 μg |
| Size of RNA Purified | All sizes, including small RNA (<200 nt) |
| Maximum Amount of Starting Material | 35 μg of RNA |
| Minimum Elution Volume | 20 μL |
| Time to Complete 10 Purifications | 20 minutes |
| Average Recovery | ≥ 90% |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 23600 (50 preps) | Cat. 43200 (100 preps) | Cat. 25100 (192 preps) | Cat. 61000 (50 preps) |
|---|---|---|---|---|
| Buffer RL | 40 mL | 2 x 40 mL | 2 x 40 mL | 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 2 x 38 mL | 38 mL |
| Elution Solution A | 6 mL | 2 x 6 mL | 2 x 20 mL | 6 mL |
| Column Activation Solution | – | – | – | 30 mL |
| Micro Spin Columns | 50 | 100 | – | – |
| Micro-Elute RNA Spin Columns | – | – | – | 50 |
| 96-Well Plate | – | – | 2 | – |
| Adhesive Tape | – | – | 4 | – |
| Collection Tubes | 50 | 100 | – | 50 |
| 96-Well Collection Plate | – | – | 2 | – |
| Elution Tubes (1.7 mL) | 50 | 100 | – | 50 |
| 96-Well Elution Plate | – | – | 2 | – |
| Product Insert | 1 | 1 | 1 | 1 |
N-(t-butyl ester-PEG4)-N-bis(PEG4-Propargyl) is a trifunctional PEG linker that combines a t-butyl ester with two terminal alkynes. The alkynes can be applied in copper-click chemistry with azides to form stable triazole linkages with a target molecule, while the t-butyl ester can be deprotected and reacted with amines or alcohols to form amides or esters.
N-(t-butyl ester-PEG4)-N-bis(PEG4-Propargyl) is a trifunctional PEG linker that combines a t-butyl ester with two terminal alkynes. The alkynes can be applied in copper-click chemistry with azides to form stable triazole linkages with a target molecule, while the t-butyl ester can be deprotected and reacted with amines or alcohols to form amides or esters.
Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 – 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. This kit can be used to isolate DNA from a broad range of viruses in urine as well. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications. The DNA is of excellent quality for various downstream applications such as PCR, qPCR and DNA fingerprinting, methylation studies and more.
This kit is fully compatible with Norgen’s Urine Collection and Preservation Tubes.
Urine DNA Isolation Kit
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 50 μL to 1.75 mL of urine. Multiple samples can be processed in 30 minutes.
Urine DNA Isolation Kit (Slurry Format)
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 3 mL to 25 mL. Multiple samples can be processed in 30 minutes. Multiple samples can be processed in 45 minutes.
Urine DNA Isolation Maxi Kit (Slurry Format)
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 25 mL of urine up to 80 mL. Multiple samples can be processed in 45 minutes.
Background
DNA found in urine can be divided into 2 basic categories. The larger species, genomic-DNA (gDNA), is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid as reported by Halicka et al. (2000). Both types of DNA can be isolated reliably using this kit.
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| Kit Specifications | |
| Minimum Urine Input | 25 mL |
| Maximum Urine Input | 80 mL |
| Time to Complete Purification | < 45 minutes |
| Size of Urine DNA Purified | Large (> 1 kb) and small (150-250 bp) |
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm up Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
| Component | Cat. 18100 (50 preps) | Cat. 48800 (50 preps) | Cat. 50100 (50 preps) |
|---|---|---|---|
| Binding Solution K | 15 mL | – | – |
| Slurry B1 | – | 18 mL | 18 mL |
| Proteinase K in Storage Buffer | 2 mL | – | – |
| Pronase K in Storage Buffer | 2 mL | – | – |
| Soluton WN | 9 mL | – | – |
| Binding Buffer A | – | – | 50 mL |
| Lysis Buffer A | – | 30 mL | 30 mL |
| Wash Solution A | – | 38 mL | 38 mL |
| Wash Solution B | 30 mL | – | – |
| Wash Solution D | 9 mL | – | – |
| Binding Solution K | 15 mL | – | – |
| Elution Buffer B | 15 mL | 15 mL | 15 mL |
| Micro Spin Columns | 50 | – | – |
| Mini Filter Spin Columns | – | 50 | 50 |
| Collection Tubes | 50 | 50 | 50 |
| Elution Tubes (1.7 mL) | 100 | 100 | 100 |
| Product Insert | 1 | 1 | 1 |
