Propargyl-PEG3-alcohol

Gel images of different ranges of library size selection. Sheared human genomic DNA was used as input.

.

Library size selection is an enrichment of a specific range of library sizes for NGS library preparations. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.

There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.

Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.

The magnetic beads, or SPRI (Solid Phase Reversible Immobilization) beads, is well used for the purification of DNA due to their reversible DNA binding. The NGS library can be size-selected by the magnetic beads or SPRI beads. The properties of the magnetic beads can be changed for a specific range of DNA binding. The contaminants and other unwanted components in the libraries can also be removed during size selection.

Specific ranges of NGS libraries can be selected using magnetic beads with different buffer compositions. The first DNA-beads binding step, also called the right-side clean-up, removes large NGS library fragments. The large NGS library fragments that bind to the beads are discarded with the beads pellet. The desired NGS library fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-DNA binding, also called the left-side clean-up. After the rinsing step, the NGS library fragments with the dual selection are eluted in water or an appropriate buffer. The magnetic beads method has great advantages over time-consuming column purification and tedious gel-based purification.

NGS library size selection with dual clean-ups.

.

Library size selection for long-read sequencing only requires a single clean-up. In this case, only the large library fragments are bound to the beads, while other small library fragments are discarded with the supernatant. The selected larger library fragments are eluted in water or an appropriate buffer after the rinsing step.

NGS library size selection with single clean-up for >5 kb and >10 kb libraries.

.

Features of NGS library size selection

• • • 5 ranges for NGS library size selection
      • • illumina PE100 sequencing: 250-350 bp
        • illumina PE150 sequencing: 300-450 bp
        • illumina PE100 sequencing: 450-750 bp
        • Long-read sequencing: >5 kb
        • Long-read sequencing: >10 kb
    • Rapid protocol: only 20 minutes
    • Simple workflow
      • • No columns required
        • No gel purification required
        • No centrifugation required

Features: 

1. Brushless frequency motor with simpler construction, more reliable performance, longer life and quietly running

2. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of  high quality steel, safe and reliable.

3. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction, safe and reliable.

4. Microprocessor control.

5. Digital display in time, speed and RCF.

6. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.

7. 3 tiers protection steel cover, stainless steel chamber, safe and reliable.

 TG20Technical Parameter：

Max. Speed	21000rpm
Max. RCF	30910×g
Max. Capacity	6×100ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤60
Temperature	Normal
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	/
Voltage(V/Hz)	AC 220V/110V  50HZ/60HZ
Size (W x D x Hmm)	513×370×320mm
Net Weight/Gross weight(Kg)	43KG/60KG
Certificates	CE,ISO & Calibration report are available

 Matched Rotors for TG20 

Order no	Rotor	Max Speed(r/min)	Volume(ml)	Max RCF(*g)
G20-1	Fixed Rotor	16000	4×8PCR	15760
G20-2	Fixed Rotor	15000	6×8PCR	21420
G20-3	Fixed Rotor	16000	8×8PCR	17480
G20-4	Fixed Rotor	15000	12×8PCR	22930
G20-5	Fixed Rotor	21000	12×1.5/2ml	30910
G20-6	Fixed Rotor	15000	40×0.5ml	22920
G20-7	Fixed Rotor	17000	24×1.5/2ml	26460
G20-8	Fixed Rotor	13500	30×1.5/2ml	19340
G20-9	Fixed Rotor	13000	48×1.5/2ml	17930
G20-10	Fixed Rotor	16000	16×5ml	22020
G20-11	Fixed Rotor	16000	6×10ml	21500
G20-12	Fixed Rotor	15000	12×10ml	22680
G20-13	Fixed Rotor	16000	12×7ml	21380
G20-14	Fixed Rotor	13000	16×10ml	19490
G20-15	Fixed Rotor	11000	12×15ml	14330
G20-16	Fixed Rotor	13000	8×15ml(conical)	17790
G20-17	Fixed Rotor	5000	24×15ml	3500
G20-18	Fixed Rotor	15000	8×20ml	22680
G20-19	Fixed Rotor	5000	30×15ml	3830
G20-20	Fixed Rotor	14000	6×30ml	19060
G20-21	Fixed Rotor	13000	6×50ml(conical)	18840
G20-22	Fixed Rotor	13000	6×50ml(round)	18730
G20-23	Fixed Rotor	13000	4×85ml	18940
G20-24	Fixed Rotor	12000	6×70ml	15570
G20-25	Fixed Rotor	12000	4×100ml	14850
G20-26	Fixed Rotor	10000	6×100ml	11380
G20-27	Fixed Rotor	18000	30×0.5ml	26660
G20-28	Swing Rotor	13000	4×5ml	14960
G20-29	Vertical Rotor	16000	16×5ml	16540
G20-30	Vertical Rotor	14000	8×30ml	16450
G20-31	Microplate Rotor	4000	2×3×48（well）	2300

Description

Description

Attogene’ s RNase Inhibitor is a protein-based ribonuclease inhibitor which noncovalently binds and inactivates a wide variety of RNases in a range of temperature (37–65°C) and pH (5.5–8.5) conditions. This product is distinct from placental RNase Inhibitor protein in that it inactivates RNases I and T1 in addition to RNases A, B and C. NA2021 is distinct from RNase Inhibitor protein in that it is less expensive, have more robust interactions with RNAses.

MADE AND FUNCTIONALLY TESTED AT ATTOGENE (MADE IN AUSTIN TEXAS – USA)

RNase Inhibitor (10,000 units)
Concentration: 40 units/μL
Volume: 0.25 mL
Storage Conditions: Store at 2°C – 8°C
Inhibits: RNases A, B, and C
Supplied in: 1X PBS with 0.05% Sodium Azide.