Introduction

This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 3g of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blot in less than 1 hour.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 3g plant and fungal tissue
Applications	PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Various plant samples (including conventional, polysaccharides and polyphenols)
Sample amount	Fresh / frozen plant samples: 2-3 gDried plant / seed samples: 0.5-1 g
Elution volume	≥500μl
Time per run	≤120 minutes
Liquid carrying volume per column	20ml
Binding yield of column	5mg

Principle

This product is based on silica Column purification. Plant material is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged.DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.

Advantages

• Broad spectrum – suitable for extracting DNA from various plant samples
• Fast – several samples can be extracted in 30 minutes by silica technology
• High purity – high quality DNA, completely remove inhibitors
• High yield – silica technology can achieve the highest yield

Kit Contents

Contents	D316302	D316303
Purification Times	10 Preps	50 Preps
RNase A	20 mg	90 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Buffer PAL	180 ml	900 ml
Buffer GWP	150 ml	800 ml
Buffer GW2	25 ml	200 ml
Buffer AE	30 ml	120 ml
HiPure DNA Maxi Columns II	10	50
50 ml Collection Tubes	20	100

Storage and Stability

RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Purchase Guide

Name	CAT NO	Fresh / frozen tissue amount	Dried tissue amount	Column type	Elution volume	Yield (muscle)
HiPure Plant DNA Mini Kit	D3187	150mg	40mg	1.5ml column	50 – 100μl	3-70μg
HiPure HP Plant DNA Maxi Kit	D3163	5g	1g	50ml column	0.7 – 3ml	75-1250μg
HiPure SF Plant DNA Kit	D3164	100mg	20mg	1.5ml column	50 – 100μl	3-50μg
HiPure SF Plant DNA 96 Kit	D3167	50mg	15mg	96 well plate	75 – 150μl	3-30μg

This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 3g of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blot in less than 1 hour.

TXK4 Blood card centrifuge, ID card Centrifuge, Gel card centrifuge

Application:

TXK4 blood group serological tests multipurpose centrifuge widely used in the test of  blood group serology, blood type,  Microcolumn agglutination, immunodetection and etc.

Features:

1. Brushless motor to make sure free maintenance,no powder polution, micro computer control and digital display the time, speed and RCF to let operation easily.

2. Special centrifugation technical to different reagent according to the requirement.

3. Regular centrifugation program, customers can press the button to choose the required one.

4. Electrical lid lock, over speed and imbalance protection.

Technical Parameters:

Max speed	4000rpm	Max RCF	2250xg
Max volume	24 cards	Noise	≤45dBA
Timer	0～99min	Net weight	23KG
Dimension(HxDxW)	483×320×265mm	Power supply	AC 220 50HZ 2A
Speed accuracy	±10rpm	Package	carton box

Matched Rotors for TXK4:

Order No.	Rotor Type	Max Speed (rpm)	Max Volume (ml)	Max RCF(g)
NO33183	Swing Rotor	3000	24 cards	1080
		4000	24 cards	2120
NO31605	Fixed Rotor	4000	30×7/5ml	2250
NO31601	Fixed Rotor	4000	12×10ml	2150

TXK4 blood group serological tests multipurpose centrifuge widely used in the test of blood group serology, blood type, Microcolumn agglutination, immunodetection and etc.