Propargyl-PEG3-bromide

TGL20 Technical Parameters
Max Speed	21000r/min	Max Volume	6×100ml
Timer	1~99h59min	Dimension	570×620×380mm
Speed Accuracy	±20r/min	Max RCF	30910×g
Noise	≤58dBA	Net Weight	84KG
Power supply	AC 220V, 50HZ 10A	Temperature Range	-20℃~40℃
Temperature Accuracy	±1℃

Matched Rotors for TGL20
Order no	Rotor	Max Speed(r/min)	Volume(ml)	Max RCF(*g)
G20-1	Fixed Rotor	16000	4×8PCR	15760
G20-2	Fixed Rotor	15000	6×8PCR	21420
G20-3	Fixed Rotor	16000	8×8PCR	17480
G20-4	Fixed Rotor	15000	12×8PCR	22930
G20-5	Fixed Rotor	21000	12×1.5/2ml	30910
G20-6	Fixed Rotor	15000	40×0.5ml	22920
G20-7	Fixed Rotor	17000	24×1.5/2ml	26460
G20-8	Fixed Rotor	13500	30×1.5/2ml	19340
G20-9	Fixed Rotor	13000	48×1.5/2ml	17930
G20-10	Fixed Rotor	16000	16×5ml	22020
G20-11	Fixed Rotor	16000	6×10ml	21500
G20-12	Fixed Rotor	15000	12×10ml	22680
G20-13	Fixed Rotor	16000	12×7ml	21380
G20-14	Fixed Rotor	13000	16×10ml	19490
G20-15	Fixed Rotor	10000	12×15ml	11840
G20-16	Fixed Rotor	13000	8×15ml(falcon)	17790
G20-17	Fixed Rotor	5000	24×15ml	3500
G20-18	Fixed Rotor	15000	8×20ml	22680
G20-19	Fixed Rotor	5000	30×15ml	3830
G20-20	Fixed Rotor	14000	6×30ml	19060
G20-21	Fixed Rotor	13000	6×50ml(falcon)	18840
G20-22	Fixed Rotor	13000	6×50ml(round)	18730
G20-23	Fixed Rotor	13000	4×85ml	18940
G20-24	Fixed Rotor	12000	6×70ml	15570
G20-25	Fixed Rotor	12000	4×100ml	14850
G20-26	Fixed Rotor	12000	6×100ml	16390
G20-27	Fixed Rotor	12000	24 pieces capillary vessel	15800
G20-28	Fixed Rotor	18000	30×0.5ml	26660
G20-29	Swing Rotor	13000	4×5ml	14960
G20-30	Vertical Rotor	16000	16×5ml	16540
G20-31	Vertical Rotor	14000	8×30ml	16450
G20-32	Microplate Rotor	4000	2×3×48（well）	2300

Features:
1. Digital display indicates the speed,time,temperature and RCF.
2. Brushless DC motor in great torque, free maintenance,no powder pollution.quick in speed up and down.
3. There are many kinds of rotors for choice.
4. Automatically electric lid lock,super speed,over temperature protection and imbalance protection.
5. The centrifuge body is made of high-quality steel,safe and reliable.

Introduction

Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.

The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen’s columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.

Details

Specifications

Features	Specifications
Recommended application	gDNA maxi yield preparation, total RNA maxi preparation
Preservation conditions	Room temperature
Stability	Up to 4 years
Filter membrane	High quality glass fiber filter GF/B, 4 layers
Membrane aperture	1.0μm
Maximum binding yield of plasmid	500 μg
Maximum yield of alcohol mediated binding	5 mg
gDNA or RNA yield (>30%ethanol)	Up to 5mg
Single liquid carrying capacity of column	20 ml
Minimum elution volume	800 μl
Withstand centrifugal force	3,000~5,000 x g
Centrifuge	Low speed centrifuge for 50mlcentrifuge tubes, >3000 x g, swing-out Rotor

Adsorption Mechanism

Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.

Ordering information

CAT.No.	Product Name	Package
C13122	HiPure gDNA Maxi Column (4 x GF/B)with 50ml Collection Tubes	100/Bag

Purchase Guide

Item No.	Product Name	Membrane type/number of layers	Collection tubes	Plasmid DNA binding capacity (Physical adsorption)	gDNA/RNA binding capacity (Alcohol-mediated adsorption)	Minimum Elution volume	Liquid volume capacity
C13010	HiPure DNA Nano Column	2 layers GF/F	2ml without cap	5μg	20μg	10μl	700μl
C13011	HiPure DNA Micro Column	3 layers GF/F	2ml without cap	10μg	50μg	15μl	700μl
C13100	HiPure DNA Mini Column I	2 layers GF/B	2ml without cap	15μg	100μg	30μl	700μl
C13110	HiPure DNA Mini Column II	4 layers GF/B	2ml without cap	35μg	200μg	50μl	800μl
C13111	HiPure RNA Mini Column	3 layers GF/B	2ml without cap	30μg	200μg	30μl	800μl
C13112	HiPure Viral Mini Column	3 layers GF/F	2ml without cap	30μg	200μg	30μl	800μl
C13113	HiPure CFDNA Mini Column	3 layers GF/F,1 layer GF/B	2ml without cap	30μg	200μg	30μl	800μl
C13120	HiPure DNA Midi Column	4 layers GF/B	15ml Centrifuge tube	125μg	1mg	500μl	4ml
C13121	HiPure DNA Midi Column III	8 layers GF/B	15ml Centrifuge tube	250μg	1mg	500μl	4ml
C13122	HiPure DNA Maxi Column	4 layers GF/B	50ml Centrifuge tube	500μg	5mg	1000μl	20ml
C13123	HiPure DNA Maxi Column III	8 layers GF/B	50ml Centrifuge tube	1mg	5mg	1000μl	20ml
C13124	HiPure DNA Maxi Column C	8 layers GF/B	50ml high speed Centrifuge tube	1mg	5mg	700μl	12ml
C13130	HiPure DNA Plate	2 layers GF/F	1.6ml Plate	30μg	100μg	80μl	900μl
C13131	HiPure gDNA Plate	2 layers GF/B	1.6ml Plate	30μg	100μg	80μl	900μl

Note: GF/B pore size is for 1.0μM glass fiber membrane; GF/F pore size is for 0.7μm glass fiber membrane.

Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.

Gel images of different ranges of library size selection. Sheared human genomic DNA was used as input.

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Library size selection is an enrichment of a specific range of library sizes for NGS library preparations. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.

There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.

Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.

The magnetic beads, or SPRI (Solid Phase Reversible Immobilization) beads, is well used for the purification of DNA due to their reversible DNA binding. The NGS library can be size-selected by the magnetic beads or SPRI beads. The properties of the magnetic beads can be changed for a specific range of DNA binding. The contaminants and other unwanted components in the libraries can also be removed during size selection.

Specific ranges of NGS libraries can be selected using magnetic beads with different buffer compositions. The first DNA-beads binding step, also called the right-side clean-up, removes large NGS library fragments. The large NGS library fragments that bind to the beads are discarded with the beads pellet. The desired NGS library fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-DNA binding, also called the left-side clean-up. After the rinsing step, the NGS library fragments with the dual selection are eluted in water or an appropriate buffer. The magnetic beads method has great advantages over time-consuming column purification and tedious gel-based purification.

NGS library size selection with dual clean-ups.

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Library size selection for long-read sequencing only requires a single clean-up. In this case, only the large library fragments are bound to the beads, while other small library fragments are discarded with the supernatant. The selected larger library fragments are eluted in water or an appropriate buffer after the rinsing step.

NGS library size selection with single clean-up for >5 kb and >10 kb libraries.

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Features of NGS library size selection

• • • 5 ranges for NGS library size selection
      • • illumina PE100 sequencing: 250-350 bp
        • illumina PE150 sequencing: 300-450 bp
        • illumina PE100 sequencing: 450-750 bp
        • Long-read sequencing: >5 kb
        • Long-read sequencing: >10 kb
    • Rapid protocol: only 20 minutes
    • Simple workflow
      • • No columns required
        • No gel purification required
        • No centrifugation required