Introduction

Usages:
For isolating lactose-fermenting Gram-negative enteric bacilli.

Principle:
Peptones are sources of nitrogen and other nutrients. Lactose is a fermentable carbohydrate. When lactose is fermented, alocal pH drop around the colony causes a color change in the pH indicator (neutral red) and bile precipitation. Bile salts,bile salts no. 3, oxgall and crystal violet are selective agents that inhibit growth of gram-positive organisms. Agar is the solidifying agent.

Formulation（per liter）:

Pancreatic Digest of Gelatin	17.0 g
Peptones (meat and casein)	3.0 g
Lactose Monohydrate	10.0 g
Sodium Chloride	5.0 g
Bile Salts	1.5 g
Agar	13.5 g
Neutral Red	30.0 mg
Crystal Violet	1 mg
Final pH	7.1±0.2

How to use：
1.Suspend 50 g in 1 L of distilled or deionized water. Heat to boiling to dissolve completely. Autoclave at 121°C for 15 minutes.

2.Transfer 1 mL of Soybean–Casein Digest Broth to 100 mL of MacConkey Broth, and incubate at 42 to 44 for 24 to 48 hours. Subculture on a plate of MacConkey Agar at 30 to 35 deg.C for 18 to 72 hours.

Quality control：
          　　

Item	The name and number of strain	PR/G	Reaction
Growth rate	E.Coli ATCC8739	PR≥0.7	Rose-red
Characteristic difference	Proteus mirabilis CMCC(b)49005	PR≥0.7	Colorless, no swarming
Selective	Staphylococcus aureus ATCC6538	G≤1	－

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of three years.

Specifications: 250g/bottle

250g

K-GLUM

SKU: 700004300

50 Assays per kit

Content:	50 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Glucomannan
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	4 to 80 μg of glucomannan per assay
Limit of Detection:	1 g/100 g
Total Assay Time:	120 min
Application examples:	Jelly sweets, cosmetics, food gums and other materials.
Method recognition:	Novel method

The Glucomannan test kit is suitable for the measurement and analysis of Glucomannan in plant products and food.

View more of our polysaccharide test kits.

Advantages

• Very cost effective 
• Only enzymatic kit available 
• Simple format 
• All reagents stable for > 2 years after preparation 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The Glucomannan test kit is suitable for the measurement and analysis of Glucomannan in plant products and food.

Introduction

This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 250-500mg soil sample
Applications	PCR, southern blot and enzyme digestion, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Soil
Sample amount	250-500mg
Elution volume	≥50μl
Time per run	≤70 minutes

Principle

This product is based on the purification method of high binding magnetic particles. Soil sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our propietary Absorber Solution. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• High concentration – maximum extraction of total DNA from soil samples
• High purity – purified DNA can be directly used in various downstream applications

Kit Contents

Contents	D635601	D635602	D635603
Purification Times	48 Preps	96 Preps	5 x 96 Preps
MagPure Particles	2.5 ml	5 ml	22 ml
2ml Bead Tubes	48	96	400
Buffer SOL	60 ml	100 ml	500 ml
Buffer SDS	6 ml	10 ml	50 ml
Reagent DX	1 ml	1 ml	5 ml
Buffer PS	10 ml	20 ml	90 ml
Absorber Solution	10 ml	20 ml	90 ml
Buffer GDP	70 ml	150 ml	2 x 350 ml
Buffer GW1*	22 ml	44 ml	220 ml
Elution Buffer	20 ml	20 ml	60 ml

Storage and Stability

MagPure Particles and Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.