Propargyl-PEG3-bromide

Propargyl-PEG5-Ms represents a bifunctional linker possessing a proparygyl group reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound as well as a mesyl group which is a good leaving group for nucleophilic reactions.

Propargyl-PEG5-Ms represents a bifunctional linker possessing a proparygyl group reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound as well as a mesyl group which is a good leaving group for nucleophilic reactions.

Overview

• Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
• Simple and quick workflow: library could be prepared in less than 5 hours
• Component of Norgen’s metagenomics workflow
• A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide 

The 16S V1-V2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V2 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The 16S V1-V2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V2 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.

Details

Supporting Data

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Storage Conditions and Product Stability
Norgen’s 16S V1-V2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

*Optical system: equipped with infinity plan achromatic stress-free metallographic objective lens,built-in polarizing ober-vation device

*Observation head device:The lens barrel can be rotated 360 degrees,and the interpupillary distance adjustment range is 50-75MM

*Eyepiece configuration:random eyepiece WF10X /22mm;

*Objective:Infinity  plan  achromatic  strain-free   metallographic  objective;5X,10X,20X,50X

*Loading  platform:Stage:Tripod  XY  composite   mechanical  moving  stage; size 140X120MM, stroke 80X50MM, minimum reading value 0.1mm

XTL-20 series metallographic microscopes are equipped with infinity plan achromatic stress-free metallographic objective lens and Wide-angle eyepiece, with the characteristics of beautiful appearance, clear imaging, broad vision, convenient operation and so on, etc. It can be widely used in biology, medicine, industry, agriculture and other fields, and is an ideal instrument for medical, teaching, scientific research and other units
Model:
XTL-20

Brand:
Yingtai