Description

Specifications

Clone	IHC409
Source	Mouse Monoclonal
Positive Control	Colon, Colon Carcinoma
Dilution Range	1:200

MutL Homolog 1 (MLH1) is a protein involved in the mismatch-repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, as the MLH1 gene is frequently mutated in patients with this cancer. Studies have shown MLH1 to be deficient in a high percentage of patients with microsatellite instability, as well as endometrial and ovarian cancers. Use of Anti-MLH1 is optimized when paired in an IHC panel with MSH6, MSH2, and PMS2. Anti-MLH1 is useful in the detection of MLH1 in a number of normal and neoplastic tissues, and for identifying a loss of MLH1 in tumors that are microsatellite-unstable.

Assay Tubes (Qubit Fluorometer)

The Qubit assay tubes are thin-wall polypropylene tubes (500 µl) for use with the Qubit Fluorometer. High-quality of the tubes guarantee the accurate measurement of different types of samples such as DNA (dsDNA, ssDNA), RNA and protein.

Qubit tubes from the same lot should be used for the experiments for optimal result. 500 tubes per package.

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Features

• • High-quality: Accurate measurement of different types of samples
  • Cost cutting: Half price as compared to other vendors

Specifications

Shipping condition	Room temperature
Type	Assay tube (for Qubit fluorometer)
For use with (equipment)	Qubit fluorometer
Storage	Store at room temperature
Package	500 tubes

Qubit is a registered trademark of ThermoFisher Scientific.

The Qubit assay tubes are thin-wall polypropylene tubes (500 µl) for use with the Qubit Fluorometer. High-quality of the tubes guarantee the accurate measurement of different types of samples such as DNA (dsDNA, ssDNA), RNA and protein.

Overview

• Detection kits for HIV
• Available in TaqMan format for analysis

Human Immunodeficiency Virus (HIV) is a retrovirus which destroys essential cells in the human immune system, such as CD4+ lymphocytes (a type of T cell), macrophages and dendritic cells. Infection with HIV therefore leads to an impaired immune system and increased susceptibility to opportunistic cancers and infections. Due to the mutating nature of HIV and damage to the T cells, the immune system is unable to fight off infections and disease. Without treatment, HIV can progress to Acquired Immunodeficiency Syndrome (AIDS). The rate of HIV progression to AIDS depends on a number of different factors including viral, host and environmental factors. However, with adherence to antiretroviral therapy (ART), many individuals with HIV will never progress to AIDS, having near normal life expectancy.   Transmission of HIV is through direct contact with infected bodily fluids, either sexually; by the sharing of blood-contaminated needles or instruments; maternally through childbirth or breastfeeding; or via blood transfusion/organ transplant. 

HIV TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

HIV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

Figure 1 / 3

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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM33750 (100 preps)	Cat. TM33710 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
HIV Primer & Probe Mix	280 μL	280 μL
HIV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1