Propargyl-PEG4-acid is a PEG reagent with an alkyne and a terminal carboxylic acid. The carboxylic acid reacts with primary amine groups with the help of activators (e.g. EDC, or HATU). The 4-unit PEG spacer increases hydrophilicity. The alkyne can participate in copper-catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Propargyl-PEG4-acid is a PEG reagent with an alkyne and a terminal carboxylic acid. The carboxylic acid reacts with primary amine groups with the help of activators (e.g. EDC, or HATU). The 4-unit PEG spacer increases hydrophilicity. The alkyne can participate in copper-catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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TG20 Table Top High Speed Centrifuge
Product Info
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Product Info
Features:
1. Brushless frequency motor with simpler construction, more reliable performance, longer life and quietly running
2. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
3. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction, safe and reliable.
4. Microprocessor control.
5. Digital display in time, speed and RCF.
6. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Pullulanase/Limit-Dextrinase
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
400
Signal Response:
Increase
Limit of Detection:
0.18 U/mL for pullulanase preparations (50-fold dilution) 0.01 U/g for limit dextrinase in milled malt
Reproducibility (%):
~ 3%
Total Assay Time:
~ 10 min (Pullanase), ~ 30 min (Limit-Dextrinase)
Application examples:
Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts.
Method recognition:
Novel method
PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-63-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.