Propargyl-PEG4-amine has a propargyl group and an amine group. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls, etc. The propargyl group can form triazole linkage with azide-bearing biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Propargyl-PEG4-amine has a propargyl group and an amine group. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls, etc. The propargyl group can form triazole linkage with azide-bearing biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
IsoPol® BST+ is a heat-tolerant BST polymerase (large fragment) with enhanced strand-displacement activity. IsoPol® BST+ is active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.
Key features
IsoPol® BST+ lacks 5’-3’- and 3’-5’-exonuclease activity.
Inactivation – IsoPol® BST+ is completely inactivated after one minute incubation at 95°C
Enhanced strand displacement and processivity.
Increased salt tolerance.
IsoPol® BST+ Glycerol FREE
Is the popular choice f or isothermal applications such as LAMP and RT-LAMP at point-of-care diagnostics for its superior amplification performance and robustness. The enzyme is offered in a 1X storage buffer at a concentration < 300 U/µl
IsoPol® BST+ High Concentration Glycerol FREE
(IsoPol® BST+ HC Glycerol FREE) is used to ease the product development processes in sequencing technologies, solid phase amplification and several isothermal technologies. Same performance as IsoPol® BST+ (i.e., high processivity, relative SDA, and relative activity) with the only difference that it is offered in a 2X storage buffer at a concentration > 500 U/
Key features
High concentration
High purity
Lyophilisation and automation friendly
Figures
Properties
Document
These DNA polymerases are ideal for Isothermal Amplification
IsoPol® BST+ is a heat-tolerant BST polymerase (large fragment) with enhanced strand-displacement activity. IsoPol® BST+ is active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.
Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.
Versatile sample input ranging from 250 µL to 30 mL
Urine Exosome Purification Mini Kit (250 µL – 1 mL urine)
Urine Exosome Purification Midi Kit (2 mL – 10 mL urine)
Urine Exosome Purification Maxi Kit (11 mL – 30 mL urine)
Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins.
No precipitation reagents, overnight incubation, protease or coagulant treatments required
No time-consuming ultracentrifugation, filtration or special syringes required
Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
Purified exosomes are compatible with functional studies.
Purified exosomes are free from any protein-bound cell-free circulating RNA
Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.
Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method.
The Urine Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different urine sample volumes ranging from 250 µL to 30 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different urine sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.
These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.
NanoSight® Analysis
Exosomes enriched with Norgen’s Urine Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration.
Exosomal RNA Analysis
Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit from exosomes enriched using Norgen’s Urine Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for Biomarker discovery.
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.
Important Note Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.
Carbendazim is a fungicide used for the treatment and control of fungal diseases. Carbendazim continues to be used, frequently in combination with other fungicides.
This kit is based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 1.5 hours in one operation, it can considerably minimize operation error and work intensity.