Propargyl-PEG4-amine

The ChIP-Seq Library Prep Kit (illumina and MGI Platform) was developed for the construction of high quality ChIP-Seq libraries using 5 ng to 400 ng of ChIP DNA as input. The kit is compatible with ChIP DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.).

ChIP-Seq Library Prep Kit Workflow

ChIP-Seq is the combination of chromatin immunoprecipitation (ChIP) with next generation sequencing. It is a powerful tool for the analysis of global transcription factors and other proteins in diseases and biological pathways, and characterization of histone modifications in a genome-wide level at single-base resolution. ChIP-Seq delivers whole genome level of functional profiling of global transcription factors, and provides better understanding of epigenetic modifications.

Three index types are available for the ChIP-Seq Library Prep Kit of the illumina platform:

Non-index (Cat.# 30032): Libraries do not have index.

Index (Cat.# 30034): Each index primer contains a unique 6-base index sequence can be used for identification. 48 samples can be pooled together. Index information can be downloaded here.

Unique dual index (Cat.# 30036): The ChIP-Seq library multiplexing for 96 samples is possible. Our unique 4-Base Difference Index System have 8 bases index length and at least 4 bases are different from each other for better library identification. Our unique dual indexing primers remove sequencing errors such as index hopping, index contamination, mis-assignment, and other errors. Index information can be downloaded here.

Indexes are available for the MGI platform kits (Cat.# 34034).

Kit advantages:

• Super fast protocol
  • Library prep can be done in 1.5 hours
  • The hands-on time is only around 10 minutes
• Easy procedure
  • Ready-to-use master mix simplified the procedure
  • Less reaction components make it easy to setup reactions
• Reduced more than half of the beads cost
• Input ChIP DNA: From 5 ng to 400 ng

Comparison of library conversion efficiency under the same condition. Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30034) was used.

Comparison of aligned reads, aligned rate and duplication rate. Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30034) was used.

Data comparison: Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30034) was used. Sequencing peak regions are shown.

The ChIP-Seq Library Prep Kit (illumina and MGI Platform) was developed for the construction of high quality ChIP-Seq libraries using 5 ng to 400 ng of ChIP DNA as input. The kit is compatible with ChIP DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.).

Name of Product

HAMA – Human Anti Mouse
Antibodies Detection Kit

Catalog Number

MQHM Z

Short Info

Lateral Flow Assay designed for the detection of Human Anti Mouse Antibodies (HAMA) in human serum.

This product is only available in the EU!

Method/Platform

Lateral Flow

Range/Assay Sensivity

97%

Test Principle

The HAMA Detection Kit is an immunoassay designed for qualitative determination of Human Anti Mouse Antibodies in human serum.

HAMA from patient’s sample bind first to mouse antibodies which are conjugated to gold nanoparticles.

In a second step the HAMA will be caught by mouse IgG antibodies, coated on the Test Line (T) of the nitrocellulose membrane.

In the presence of the HAMA-conjugat-complex  a band becomes visible at the location of the Test Line (T).

The surplus of gold particles continues to migrate through the membrane and is captured at the Control Line (C) by specific antibodies.

Color’s intensitiy of the test line is directly proportional to the HAMA concentration in the sample.

Brief Instructions

Storage

2-8°C

Components

HAMA Test Unit, Chase Buffer, Evaluation Cards

5 tests
Lateral Flow Assay designed for the detection of Human Anti Mouse Antibodies (HAMA) in human serum.

1 kb DNA Ladder in 1% agarose gel

• For sizing and quantification of double strand DNA fragments.
• Composed of ten bands as shown on right.
• The 4 kb band with higher concentration is easily distinguishable from the others.
• Premixed with 6X DNA loading buffer for direct gel loading.

1 kb DNA Ladder in 1% agarose gel

• For sizing and quantification of double strand DNA fragments.
• Composed of ten bands as shown on right.
• The 4 kb band with higher concentration is easily distinguishable from the others.
• Premixed with 6X DNA loading buffer for direct gel loading.