Propargyl-PEG4-amine has a propargyl group and an amine group. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls, etc. The propargyl group can form triazole linkage with azide-bearing biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Propargyl-PEG4-amine has a propargyl group and an amine group. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls, etc. The propargyl group can form triazole linkage with azide-bearing biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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022070P1 Plate Count Agar
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Product Info
Introduction
Usages: For determination of total bacterial count.
Principle: Tryptone provide carbon and nitrogen; yeast extract powder provides B vitamins; glucose to provide energy, agar is the solidifying agent.
Formulation(per liter): Tryptone 5.0g Yeast extract powder 2.5g Glucose 1.0g Agar 15.0g Final pH 7.0 ± 0.2
How to use: 1.Suspend 23.5g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min. 2.Diluted and treated samples.
Quality control:
Item
The name and number of strain
Growth
Colony
1
E.Coli ATCC8739
Good
white
2
Staphylococcus aureus ATCC6538
Good
yellow
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Plasma/Serum Exosome and Free-Circulating RNA Isolation Kits
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Overview
Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
Versatile sample input ranges
Isolate all sizes of free-circulating RNA, including microRNA
The purified exosomal RNA is free from any circulating RNA-binding proteins
No phenol extractions, Proteinase K treatment, nor carrier RNA
No time-consuming ultracentrifugation, filtration nor special syringes are required
No precipitation reagents, nor overnight incubation required
Concentrate isolated exosomal RNA and are free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA as well as all sizes of the free-circulating protein-bound RNA, including microRNA. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, these kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit
For sample volumes ranging from 50 µL to 1 mL.
Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit
For sample volumes ranging from 1 mL to 4 mL.
Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50 – 100 µL
Time to Complete 10 Purifications
40 – 45 minutes
Average Yields*
Variable depending on specimen
*Please check page 5 of the product insert for the average yields and the common RNA quantification methods
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Important Note This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
Ideal magnet for all of your low volume needs. From Low elution 96-well PCR applications to 384-well, the X396 has you covered. No need to purchase two separate plates anymore. 96-well volumes as low as 5 µL are achieved from 96-well, PCR plates on the inside of the magnet, while the outside of each magnet will pull beads to well sides in 384-well plates.
ANSI/ SBS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot on bottom. ANSI/SBS footprint on top to accept all common microplates.
Integrated Cushion base for maximum recovery. Helps aid in set-up, robot positioning inconsistencies, and labware consumable inconsistencies
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
Ideal magnet for all of your low volume needs. From Low elution 96-well PCR applications to 384-well, the X396 has you covered. No need to purchase two separate plates anymore. 96-well volumes as low as 5 µL are achieved from 96-well, PCR plates on the inside of the magnet, while the outside of each magnet will pull beads to well sides in 384-well plates.