Propargyl-PEG4-CH2CO2-NHS

Permagen’s  50 mL Centrifuge Tube Magnetic rack is designed for magnetic bead separations from up to six, 50 mL Centrifuge tubes

Accommodates any common 50 mL Centrifuge Tubes

Beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet

Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads

SKU #

MSR6X50

Features

• Aluminum alloy design for years of trouble-free use
• Fast magnetic bead separations
• Held to tighter tolerances for more consistent results
• Pulls beads to side of wells for easy aspiration without disturbing bead pellet 
• Rubber feet to help prevent slippage
• Stable design
• Four magnets per tube 

Compatibility

• Up to six 50 mL Centrifuge Tubes
• Any Magnetic Beads

Volumes

Maximum Volume – 50 mL

Minimum Volume – 10 mL

Permagen’s 50 mL Centrifuge Tube Magnetic rack is designed for magnetic bead separations from up to six, 50 mL Centrifuge tubes

Opentrons OT-2 Tips, 20µL. Optimized for volumes 1µL to 20µL. Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.

Compatible with: Opentrons 8-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN1 P10 Electronic Pipettes, Opentrons 8-Channel GEN1 P10 Electronic PipettesOpentrons platform and workstation accuracy and precision performance is not validated with use of non-Opentrons tips.

Opentrons OT-2 Tips, 20µL. Optimized for volumes 1µL to 20µL. Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.

Compatible with: Opentrons 8-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN1 P10 Electronic Pipettes, Opentrons 8-Channel GEN1 P10 Electronic PipettesOpentrons platform and workstation accuracy and precision performance is not validated with use of non-Opentrons tips.

Overview

• Purify amplified DNA ranging from 100 bp -15,000 bp in size
• Fast and efficient spin column format 
• Available in a 50 prep size and a 250 prep size
• Also available in 96 well format
   

This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.

The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Details

Supporting Data

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Kit Specifications – Spin Column
Column Binding Capacity	10 μg
Size of DNA Purified	100 – 15,000 bp
Average Recovery	> 90%
Average Primer Removal	> 90%
Minimum Elution Volume	30 μL
Time to Complete 10 Purifications	15 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 14400 (50 preps)	Cat. 45700 (250 preps)	Cat. 24800 (192 preps)
Binding Buffer C	30 mL	5 x 30 mL	3 x 30 mL
Wash Solution A	12 mL	2 x 20 mL	2 x 38 mL
Elution Buffer B	8 mL	2 x 30 mL	2 x 15 mL
Spin Columns	50	250	–
Collection Tubes	50	250	–
96-Well Plate	–	–	2
Adhesive Tape	–	–	4
96-Well Collection Plate	–	–	2
Elution Tubes (1.7 mL)	50	250	–
96-Well Elution Plate	–	–	2
Product Insert	1	1	2