Propargyl-PEG4-Maleimide has a propargyl group and a maleimide group. The propargyl group reacts with azide compounds or biomolecules under the catalyzation of copper in Click Chemistry reactions. The maleimide group enables the formation of a covalent bond with biomolecules containing a thiol group. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG4-Maleimide has a propargyl group and a maleimide group. The propargyl group reacts with azide compounds or biomolecules under the catalyzation of copper in Click Chemistry reactions. The maleimide group enables the formation of a covalent bond with biomolecules containing a thiol group. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Reagent technology of protein/enzyme system: freeze-dried powder, freeze-dried microspheres
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This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39ºC~42ºC), with the help of auxiliary proteins and single-strand binding proteins,the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins;along with the dissociation of the recombinase from the complex,the polymerase also binds to the 3′ end of the primer and begins chain extension.It is suitable for laboratory-level DNA amplification and DNA amplification for other detection purposes.
Usages: For the growth of pathogenic and non-pathogenic microorganisms.
Principle: Polypeptone provide carbon and nitrogen sources, vitamins and growth factors; Neisseria starch can promote the growth characteristics and enhanced hemolytic streptococci; sodium chloride to maintain osmotic balance; agar as culture medium coagulant.
Formulation (per liter): Peptone: 10g Beef extract powder :10g Sodium chloride: 5g Agar :15g Final pH 7.4 ± 0.2
How to use: 1. Suspend 40g of the product, adding 1 L of distilled or deionized water, ,heated to boiling stirring until completely dissolved, dispensing into flask, 121 autoclaved 15min. 2.After the medium is cooled to about 50 , sterile procedures, basal medium was added per 100mL defibrinated sheep or rabbit blood 5-10mL, shake pour plate; also dispensing sterile tubes set into the slope. Spare. 3. take the bacteria to be tested fresh pure cultures inoculated crossed or coated on the plate, set the temperature of the incubator training requirements were cultured for a predetermined time. 4. Observe the results.
Quality control: The following were inoculated after 36 ± 1 for 24h , the results are as follows: bacteria name bacteria No. growth status colony characteristics Escherichia coli ATCC25922 not good hemolysis
Staphylococcus aureus, ATCC6538 good β-hemolytic
Beta-hemolytic streptococcus CMCC (B) 32210 good β-hemolytic
Streptococcus pneumoniae CMCC (B) 31001 good α-hemolytic
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
DBCO-PEG36-TFP ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules. The TFP ester is less susceptible to undergo hydrolysis compared to the NHS ester. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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DBCO-PEG36-TFP ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules. The TFP ester is less susceptible to undergo hydrolysis compared to the NHS ester. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.