Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue, whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from blood, tissue, culture cells, swab, blood spots using 96 plate
Applications	PCR, southern bolt and virus detection, etc
Purification method	96 well plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Blood, serum, plasma, milk, saliva, and other liquid samples and cultured cells
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column

Principle

This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
• High throughput – 96 samples can be processed simultaneously

Kit Contents

Contents	D311701	D311702
Purification Times	1 x 96	4 x 96
HiPure gDNA Plate	1	4
96 well Plate (2.2ml)	1	4
1.6ml Collection Plate	1	4
0.5ml Collection Plate	1	4
Silicon Seal Tape	1	4
Seal Film	5	25
Buffer ATL	30 ml	100 ml
Buffer AL	30 ml	100 ml
Buffer DW1	60 ml	250 ml
Buffer GW2	50 ml	2 x 100 ml
Proteinase K	50 ml	200 ml
Protease Dissolve Buffer	5 ml	15 ml
Buffer AE	30 ml	120 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Name	CAT NO	Sample amount	Leukocyte protocol*	Colum type	Elutio volume	Average yield	Time per run
HiPure Blood DNA Mini Kit	D3111	10-200μl	1ml	2ml column	≥20μl	5-9μg/200μl	≤30 minutes
HiPure Tissue&Blood DNA Midi Kit	D3113	0.2-2ml	10ml	1.5ml column	≥300μl	20-40μg/1m	≤80 minutes
HiPure Tissue&Blood DNA Maxi Kit	D3115	3 -10ml	10ml	15ml column	≥700μl	20-40μg/1m	≤90 minutes
HiPure Tissue&Blood DNA 96 Kit	D3117	1-200μl	1ml	96 well plate		3-8μg/200μl

*Note：Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue, whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

DD6 Floor Standing 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

Application 

for DD6 Floor standing 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

Widely used in the area of laboratory, pharmaceutical factory, biochemistry, biological products and so on. It is the ideal instruments for blood separation, protein precipitation and cell collection.

Features 

for DD6 Floor standing 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

1. Brushless DC motor in great torque, free maintenance, no carbon dust pollution, quick in speed up and down.
2. Flexible axle driven system which drive the rotor directly, smooth in operation, low noise and small vibration.
3. Automatically electric lid lock, super speed, over temperature protection and imbalance protection.
4. Microprocessor control, speed, time, temperature and RCF in operation, speed rising and reducing quick, operate simply.
5.10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
6. There are many rotors for your choice, adapters are available by experiment requirements.
7.3 tiers protection steel cover, safe and reliable.

Technical Parameters 

for 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

Max Speed: 6000r/min	Max.RCF: 6600xg
Max Volume: 4x1000ml	Power supply:AC220V,50HZ,10A
Timer: 1~99min	Noise:≤55dBA
Dimension:800×645×830mm	Net Weight: 170KG
Speed Accuracy: ±20r/min	Package(W*D*H): 920×800×1320mm
Temperature range:	Inapplicable

Matched Rotors for DD6 laboratory centrifuge

Order No.	Rotor type	Max.Speed (rpm)	Max.Volume (ml)	Max.RCF (xg)
No33097	Swing out rotor	4,000	4x1000ml (round/square cup )	4,060
No30221	Angle rotor	6,000	4x300ml	5,390
No30222	Angle rotor	6,000	6x300ml	5,660
No30223	Angle rotor	6,000	6x500ml	6,600

Packing & Shipping_____________________

by Sea/ by Air / by Express Sevices DHL, Fedex, TNT…

DD6 is floor standing large capacity 4x1000ml, 6x300ml, 6x500ml laboratory centrifuge machine without refrigerated one. It is up to max.speed 6000rpm.

Overview

• Detection kits for Cryptosporidium
• CE-IVDD marked in accordance with the European Commission Directive 98/79/EC.
• Research version available
• Available in Taqman Probe format for analysis

Cryptosporidium is a parasite found in water that causes an infection in mammals termed cryptosporidiosis. It is one of the most common water-borne diseases and is found world-wide. It affects the intestines of mammals and typically causes an acute short-term infection. The most common symptom is self-limiting diarrhea in healthy individuals, however in immunocompromised individuals the symptoms are particularly severe and often fatal. There is no specific treatment for cryptosporidiosis other than fluid rehydration and management of any pain. Therefore early detection of Cryptosporidium in water is the foremost action to prevent the infection.

Cryptosporidium TaqMan RT-PCR Kit, 24 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Details

Supporting Data

Figure 1 / 3

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Storage Conditions

The Cryptosporidium TaqMan PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. DxTM39100 (24 rxns)
MDx TaqMan 2X RT-PCR Master Mix Dx	550 µL
Cryptosporidium Primer & Probe Mix Dx	2 x 70 µL
Cryptosporidium Positive Control Dx – 200,000 μL	50 µL
Nuclease-Free Water	2 x 1.25 mL
Product Insert	1