Propargyl-PEG4-t-butyl ester

Overview

• Rapid and simple procedure
• Excellent quality and yield of DNA
• Process a broad spectrum of plant species and filamentous fungi
• Isolate total DNA including pathogen DNA without phenol 
• Available in spin column format and 96-well format for high throughput applications

These kits provide a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.

Plant/Fungi DNA Isolation Kit (Spin Column)

Complete 10 purifications in 45 minutes. This kit offers a maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.

Plant/Fungi DNA Isolation 96-Well Kit (High Throughput)

For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system. Complete 96 purifications in 50 minutes. This kit offers a maximum loading volume of 500 μL per well, and a maximum binding capacity of 50 μg per well.

Plant/Fungi DNA Isolation Kit (Magnetic Bead System)

The DNA is bound to the surface of the magnetic beads under optimized buffer conditions and released using a low salt buffer system. The Plant DNA Isolation Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.

Plant/Fungi DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Details

Supporting Data

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Kit Specifications – Spin Column
Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Maximum Amount of Starting Material:Plant TissuesFungi (wet weight)	100 mg 100 mg
Average Yields* 50 mg Tomato Leaves 50 mg Grape Leaves 50 mg Peach Leaves 50 mg Plum Leaves 50 mg Pine Needles Botrytis cinerea (50 mg wet weight) Fusarium sp. (50 mg wet weight) Aspergillus niger (50 mg wet weight)	18 µg 10 µg 10 µg 10 µg 5 µg 1.5 µg 2 µg 4 µg
Time to Complete 10 Purifications	45 minutes

* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
 

* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage. 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. This kit is stable for 1 year after the date of shipment.

Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits

Plants	Plants (Cont’d)	Fungi
Tomato	Turnip	Aspergillus niger
Grape	Chinese Cabbage	Mucor racemosus
Peach	Radish	Cladosporium cladosporioides
Plum	Komatsuna	Fusarium oxysporum
Pine Needles	Apricot	Penicillium sp.
Raspberry	Sweet Potato	Botrytis cinerea (Botryotinia fuckeliana)
Strawberry	Hydrangea	Pichia sp.
Legumes	Fig	Rhizopus oryzae
Prosopis cineraria (Ghaf)	Turf	Alternaria tenuissima
Sorghum grass	Cherry	Fusarium sp.
Tobacco	Saintpaulia
Arabidopsis	Lotus
Lichen	Carrot
Corn seed	Hansen
Sunflower seed	Pistachio
Olive seed
Soybean seed

Component	Cat. 26200 (50 preps)	Cat. 26250 (250 preps)	Cat. 26900 (192 preps)	Cat. 58200 (50 preps)	Cat. 62400 (192 preps)
Lysis Buffer L	30 mL	1 x 30 mL 2 x 60 mL	2 x 60 mL	60 mL	2 x 60 mL
Binding Buffer I	7 mL	1 x 7 mL 1 x 25 mL	25 mL	7 mL	25 mL
Solution WN	18 mL	1 x 18 mL 1 x 55 mL	55 mL	18 mL	55 mL
Wash Solution A	38 mL	2 x 38 mL	2 x 38 mL	–	–
Elution Buffer B	15 mL	30 mL	30 mL	8 mL	30 mL
RNAse A	1 vial (80 μL)	5 vials (5 x 80 μL)	1 vial	1 vial	1 vial
Magnetic Bead Suspension	–	–	–	4 x 1.1 mL	2 x 8.5 mL
Filter Columns	50	250	–	–	–
Spin Columns	50	250	–	–	–
Collection Tubes	100	500	–	–	–
96-Well Plate	–	–	2	–	2
96-Well Collection Plate	–	–	2	–	–
Adhesive Tape	–	–	4	–	2
Elution Tubes (1.7 mL)	50	250	–	50	–
96-Well Elution Plate	–	–	2	–	2
Product Insert	1	1	1	1	1

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings

K-ARGA

SKU: 700004264

115 assays (manual) / 1150 assays (microplate) / 1150 assays (auto-analyser)

Content:	115 assays (manual) / 1150 assays (microplate) / 1150 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	L-Arabinose, D-Galactose
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	4 to 80 μg of L-arabinose or D-galactose per assay
Limit of Detection:	0.58 mg/L (L-arabinose), 0.69 mg/L (D-galactose)
Reaction Time (min):	~ 12 min (L-arabinose), ~ 6 min (D-galactose)
Application examples:	Analysis of hydrolysates of oligo- and polysaccharides (e.g. arabinan, arabinoxylan, galactan, arabinogalactan), milk, dairy products, foods containing milk (e.g. dietetic foods, bakery products, baby food, chocolate, sweets and ice-cream), food additives (e.g. sweeteners), cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Novel method

The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

See more of our monosaccharide assay kits.

Advantages

• Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.
• Very rapid reaction due to inclusion of galactose mutarotase (patented technology) 
• Very cost effective 
• All reagents stable for > 2 years after preparation 
• Only enzymatic kit available 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included 
• Suitable for manual, microplate and auto-analyser formats

The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products.