Propargyl-PEG5-acid

Name of Product

Schistosoma mansoni – IgG ELISA

Catalog Number

AF 9600

Short Info

This ELISA kit is intended for the quantitative detection of IgG antibodies against Schistosoma mansoni and Schistosoma haematobium in human serum.

This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Method/Platform

ELISA in microplate format

Range/Assay Sensivity

pNPP, λ=405 nm

Test Principle

Specific antibodies in the sample will bind to Schistosoma mansoni antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A alkaline phosphatase conjugate.

Brief Instructions

Storage

2-8°C

Components

ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, enzyme buffer, stopping solution

12 x 8 strips (96 tests)
This ELISA kit is intended for the quantitative detection of IgG antibodies against Schistosoma mansoni and Schistosoma haematobium in human serum.

Description 

Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels. 

Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.

Key Features

• User-friendly gel cassette:
  • Numbered and framed wells for sample loading
  • With cassette opener for easy use
• Enhanced gel performance:
  • Enhanced band sharpness
  • Better resolution of small proteins
  • Stable for shipping at ambient temperature
• Easy compatibility: 
  • Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
  • Compatible with most popular protein electrophoresis systems 

Storage and stability

Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.

Do not freeze Q-PAGE™ Precast Gels Remove tape and comb before electrophoresis. 

Keep Q-PAGE™ Precast Gels flat during storage.

Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.

Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.

Overview

• Extract high quality & quantity total RNA including miRNA
• No phenol step required – isolate all RNA in one fraction
• Rapid processing in under 40 minutes
• Isolate total RNA from a wide variety of specimens
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants, and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen’s Purification Technology

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Buffer RL (please see the flow chart on page 4). Ethanol is then added to the lysate, and the solution is loaded onto a maxi spin column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Solution A. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.

Details

Supporting Data

Figure 1 / 2

Previous

Next

Click for expanded view

Kit Specifications
Maximum Column Binding Capacity	1.5 mg
Maximum Column Loading Volume	20 mL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material
Liver Tissue	100 mg
Heart Tissue	50 mg
Kidney Tissue	100 mg
Brain Tissue	250 mg
Lung Tissue	100 mg
Whole Blood	2 – 10 mL*
Bacteria	2.5 x 10 10 cells
Yeast	2 x 108 cells
Fungi	1 g
Plant Tissues	1 g
Time to Complete 4 Purifications	40 minutes
Average Yield: HeLa Cells (5 x 107 cells) E. coli (2.5 x 1010 cells)	~750 μg ~1.5 mg

*For isolating total RNA from purified leukocytes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 26800 (8 preps)
Buffer RL	2 x 40 mL
Wash Solution A	4 x 38 mL
Elution Solution A	3 x 20 mL
RNA Maxi Spin Columns (with collection tubes)	8
Elution Tubes (50 mL)	8
Product Insert	1