Introduction

A highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. This kit can be easily used in manual and automated 96 or 384-well formats.

Details

Specifications

Features	Specifications
Main Functions	Recover 100-400μl DNA/ RNA from PCR products / enzymatic reaction solution / or crude DNA / RNA
Applications	Automatic sequencing, enzyme digestion, PCR and labeling
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Agarose Gel, PCR products, enzymatic reaction solution
Sample amount	100-400µl
Recovery	80%
Elution volume	≥30μl

Principle

The MagPure method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments 100bp and larger toparamagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.

Advantages

• High recovery efficiency – up to 80% DNA recovery

Kit Contents

Contents	D500301	D500302	D500303
Purification Times	50 Preps	500 Preps	5000 Preps
Buffer AL	10 ml	60 ml	550 ml
Buffer BD*	5 ml	25 ml	2 x 100 ml
MagPure RNA Particles	1.2 ml	12 ml	120 ml
RNase Free Water	5 ml	30 ml	250 ml

Storage and Stability

MagPure RNA Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

A highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. This kit can be easily used in manual and automated 96 or 384-well formats.

Introduction

The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA and miRNA from cell and tissue without MagZol reagent
Applications	RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Cell, Animal tissue, Plant tissue
Sample amount	Cells: ≤ 5 x 10^6, Animal tissue:<10mg
Elution volume	≥30μl
Time per run	≤40 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

Biological samples are first lysed and homogenized in a highly denaturing guanidine isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.

Advantages

• High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• Fast – several samples can be extracted in 40 minutes
• High applicability – samples including animals, plants, bacteria, cells, etc.
• High concentration – efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity
• Safe – no phenol chloroform extraction and no use of Trizol reagent

Kit Contents

Contents	R431102	R431103
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	100	2 x 250
2ml Collection Tubes	100	2 x 250
Proteinase K	48 mg	240 mg
Protease Dissolve Buffer	5 ml	15 ml
Buffer RLC	40 ml	200 ml
Buffer RWC	20 ml	80 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	60 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.

Overview

Optically clear heat sealing film which is non-peelable and non-pierceable. The seal is suitable for qPCR, optical applications and storage.

• Heat sealing offers a 100% effective method for plate sealing for a complete seal integrity, as well as being quick and cost effective
• Our ClearASeal Weld is an optically clear polymer film forming a permanent seal to polypropylene plates
• The optical clarity of this seal enables its use for sealing plates required for imaging use, including fluorescent detection methods such as qPCR and colorimetric assays
• The ClearASeal Weld forms a complete seal to a plate, enabling both low and very high temperature uses, including low temperature storage and high temperature incubations
• This seal is suitable for PCR/qPCR, even without the use of a pressurized heated lid, and is 100% effective when used in water bath thermal cyclers
• The permanent nature of this 100% effective seal renders it suitable for the storage and disposal of hazardous material
• ClearASeal Weld demonstrates a good solvent resistance and can be utilized for long term compound storage
• This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
• Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT

Optically clear heat sealing film which is non-peelable and non-pierceable. The seal is suitable for qPCR, optical applications and storage.