Propargyl-PEG5-amine is a propargyl linker that is commonly used as a Click Chemistry reagent for copper-catalyzed reactions with azides. The amine moiety reacts with carboxylic acids, activated NHS esters and other carbonyl compounds. The PEG spacer helps improve the water solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG5-amine is a propargyl linker that is commonly used as a Click Chemistry reagent for copper-catalyzed reactions with azides. The amine moiety reacts with carboxylic acids, activated NHS esters and other carbonyl compounds. The PEG spacer helps improve the water solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Solid Phase Reversible Immobilization magnetic beads are often used for DNA purification because they are simple, fast, and effective. The beads are paramagnetic particles coated with carboxyl groups that reversibly bind to nucleic acid. However, Standard SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered by SPRI beads. We have developed Magnetic Beads (microRNA & Oligo Purification) to solve the problem.
With our proprietary beads technology, the beads overcomes the hurdle of the short DNA/RNA recovery problem. The magnetic beads is ideal for microRNA purification, oligo purification, short DNA/RNA purification, and removing impurities and unwanted components such as dNTPs, detergents, salts, proteins, and other contaminants effectively. The magnetic bead reagents are RNase-free and can be used for both DNA and RNA applications.
Our magnetic beads are optimized for microRNA purification, oligo purification, and DNA/RNA purification. The fragments can be as short as 20 bases, such as microRNA, tRNA, dsDNA fragments 20 bp or longer, ssDNA fragments 20 nt or longer, RNA fragments 20 nt or longer, DNA/RNA hybrid fragments 20 bp or longer, and oligos and chimeric oligos 20 nt or longer. Purified short DNA and RNA fragments are ideal for applications requiring high-quality fragments, as the fragments are free of impurities and contaminants.
Comparison of short DNA fragments recovery. BioDynami 20 bp DNA ladder (Cat. # 10002) was used as DNA input. Ampure XP beads, BioDynami beads (DNA & RNA purification) and BioDynami beads (microRNA & Oligo purification) were tested. Only BioDynami beads (microRNA & Oligo purification) successfully recovered DNA fragments between 20-100 bp.
Recovery of DNA and RNA oligos with BioDynami magnetic Beads (microRNA & Oligo Purification). 20 nt of DNA oligos and RNA oligos were used as input. Input and recovered oligos were quantified with BioDynami ssDNA Quantification kit (Cat. # 40043) and BioDynami RNA Quantification kit (Cat. # 40044).
Features
This product is specially designed for DNA extraction from forensic samples such as bone, hair, fingernail, seminal strain, dry blood spot, ect. Especially designed for forensic detection. The obtained DNA can be directly used for PCR, STR detection and other down stream applications.
| Contents | D635901D | D635902D | D635903D |
| Purification Times | 48 | 96 | 480 |
| MagPure Particles N | 1.1 ml | 2.2 ml | 11 ml |
| Buffer ATL | 40 ml | 80 ml | 2 x 200 ml |
| Buffer BGL | 40 ml | 80 ml | 2 x 200 ml |
| Buffer BST1 | 60 ml | 120 ml | 550 ml |
| Buffer GW2* | 20 ml | 25 ml | 2 x 50 ml |
| Proteinase K | 48 mg | 110 mg | 2 x 220 mg |
| Protease Dissolve Buffer | 5 ml | 10 ml | 30 ml |
| DTT | 235 mg | 235 mg | 3 x 235 mg |
| Elution Buffer | 10 ml | 10 ml | 30 ml |
32/48 channel machine
| Product | Contents and volume | D6359D-TL-06 |
| Buffer BGL | 80 ml | |
| Buffer ATL | 80 ml | |
| Proteinase K | 110 mg | |
| Protease Dissolve Buffer | 10 ml | |
| DTT Powder | 2 x 235 mg | |
| Elution Buffer | 5 ml | |
| AS-Tip | 12 pcs | |
| 2.0ml V bottom plate | Row 1/7：empty | 6 plates |
| Row 2/8：500μl Buffer BST1 | ||
| Row 3/9：500μl Buffer BST1 | ||
| Row 4/10：20μl MagPure Particles N 500μl Buffer GW2 Row 5/11：500μl Buffer GW2 (ethanol ~350ul) | ||
| Row 6/12：60μl Elution Buffer |
96 channel machine
| Product | Contents and volume | D6359D-F-96 | D6359D-F-48 |
| Buffer BGL | 80 ml | 40 ml | |
| Buffer ATL | 80 ml | 40 ml | |
| Proteinase K | 110 mg | 48 mg | |
| Protease Dissolve Buffer | 10 ml | 5 ml | |
| DTT Powder | 235 mg | 235 mg | |
| Elution Buffer | 5 ml | 5 ml | |
| 96-Tip (AS) | 1 | 1 | |
| Sample Plate 1 | 500μl Buffer BST1 | 1 | 1 |
| Sample Plate 2 | 500μl Buffer BST1 | 1 | 1 |
| Wash Plate 1 | 20μl MagPure Particles N500μl Buffer GW2 | 1 | 1 |
| Wash Plate 2 | 500μl Buffer GW2 | 1 | 1 |
| Elute Plate | 60μl Elution Buffer | 1 | 1 |
Storage and Stability
Proteinase K and DTT Powder should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these condition.
This product is specially designed for DNA extraction from forensic samples such as bone, hair, fingernail, seminal strain, dry blood spot, ect. Especially designed for forensic detection. The obtained DNA can be directly used for PCR, STR detection and other down stream applications.
K-RHAMNOSE
SKU: 700004334
50 / 100 assays (manual) / 550 assays (microplate) / 550 assays (auto-analyser)
| Content: | 50 / 100 assays (manual) / 550 assays (microplate) / 550 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 1 year under recommended storage conditions |
| Analyte: | L-Rhamnose |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 5 to 100 µg of L-rhamnose per assay |
| Limit of Detection: | ~ 1.2 mg/L |
| Reaction Time (min): | ~ 5 min at 25oC or ~ 4 min at 37oC |
| Application examples: | Hydrolysates of plant material and polysaccharides, culture media / supernatants and other materials. |
| Method recognition: | Novel method |
The L-Rhamnose Assay Kit for the measurement of L-rhamnose in plant extracts, culture media/supernatants and other materials is a simple, rapid method.
L-Rhamnose occurs naturally in the L-form and is commonly present as a component of the carbohydrate moiety of eukaryotic glycoproteins and in plant cell wall polysaccharides. The most abundant occurrence of L-rhamnose is within the pectic fraction of plant cell wall polysaccharides. L-Rhamnose is commonly used as a non-metabolisable marker along with lactulose for dual-permeability testing in the diagnosis of intestinal diseases such as Crohn’s disease or coeliac disease.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
View more of our monosaccharide and disaccharide assay kits.
Advantages
The L-Rhamnose Assay Kit for the measurement of L-rhamnose in plant extracts, culture media/supernatants and other materials is a simple, rapid method.
