Propargyl-PEG5-CH2CO2H is an alkyne linker with a carboxylic acid. The carboxylic acid can react with primary amines to form stable amide bonds; activator (e.g. EDC, or HATU) is needed. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry to form stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG5-CH2CO2H is an alkyne linker with a carboxylic acid. The carboxylic acid can react with primary amines to form stable amide bonds; activator (e.g. EDC, or HATU) is needed. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry to form stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
General information
Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.
Kit contents
Shipping condition
Throughout the shipping process, the temperature is maintained under -70°C.
Storage and expiration
Champion™ Competent Cells must be stored between -70°C to -80°C. Subsequent freeze-thaw cycles will reduce transformation efficiency. If high efficiency is required for the experiment, do not use aliquots that have gone through several freeze-thaw cycles. The efficiency of Champion™ Competent Cells lasts for 1 year with proper storage.
Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.
K-INTDF
SKU: 700004305
100 assays per kit
| Content: | 100 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Dietary Fiber |
| Assay Format: | Enzymatic |
| Detection Method: | Gravimetric/HPLC |
| Signal Response: | Increase |
| Limit of Detection: | 0.5 g/100 g |
| Total Assay Time: | ~ 3 h work (over 2 days) |
| Application examples: | Food ingredients, food products and other materials. |
| Method recognition: | AACC Method 32-45.01, AACC Method 32-50.01, AOAC Method 2009.01 and AOAC Method 2011.25 |
The Integrated Total Dietary Fiber test kit is suitable for the measurement and analysis of Integrated Total Dietary Fiber.
Updated to include resistant, branched maltodextrins as a component in dietary fiber.
K-INTDF – An Integrated Procedure (AOAC Method 2009.01 & 2011.25) for the measurement of Total Dietary Fiber (including resistant starch and non-digestible oligosaccharides). This method combines the key attributes of AOAC Official Methods of Analysis 2002.02, 985.29, 991.43, 2001.03.
Specific dietary fiber fractions are measured as follows:
1. Insoluble dietary fiber (IDF), Higher Molecular Weight Soluble Dietary Fiber (SDFP) and Lower Molecular Weight Soluble Dietary Fiber (SDFS) determination (AOAC Method 2011.25)
2. Total High Molecular Weight Dietary Fiber (HMWDF) and SDFS determination (AOAC Method 2009.01)
The enzymes used in these methods are of very high purity; they are effectively devoid of contaminating enzymes active on β-glucan, pectin and arabinoxylan.
Data calculators are located in the Documents tab.
See our full range of dietary fiber and starch products.
Validation of Methods
Advantages
The Integrated Total Dietary Fiber test kit is suitable for the measurement and analysis of Integrated Total Dietary Fiber.
endo-BCN-PEG3-acid is a click chemistry linker consisting of a BCN group with a terminal carboxylic acid. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to yield a amide bond. The BCN group can react with azide-tagged biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
endo-BCN-PEG3-acid is a click chemistry linker consisting of a BCN group with a terminal carboxylic acid. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to yield a amide bond. The BCN group can react with azide-tagged biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
