Propargyl-PEG5-CH2CO2H is an alkyne linker with a carboxylic acid. The carboxylic acid can react with primary amines to form stable amide bonds; activator (e.g. EDC, or HATU) is needed. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry to form stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG5-CH2CO2H is an alkyne linker with a carboxylic acid. The carboxylic acid can react with primary amines to form stable amide bonds; activator (e.g. EDC, or HATU) is needed. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry to form stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This kit is used for extracting total pathongen nucleic acid from low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate, culture, etc. The purified DNA/RNA is ready for downstream clinical in vitro detection such as Real Time PCR, biochip analysis, NGS and other related detection.
Specifications
| Features | Specifications |
| Main Functions | Extract pathogen DNA/RNA from whole blood, body fluid, serum, plasma, sputum, immersion solution, tissue homogenate supernatant, etc. |
| Applications | RT-PCR，PCR，NGS |
| Products | Pathogen DNA and RNA |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Whole blood, plasma, serum, immersion solution, tissue homogenate supernatant |
| Sample amount | 200μl |
| Elution volume | ≥30μl |
| Time per run | 30 mins |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA is finally eluted with low-salt buffer (10 MmTris, pH 8.0).
Advantages
Kit Contents
| Contents | IVD4179 |
| Purification Times | 50 Preps |
| HiPure Viral Mini Column | 50 |
| 2ml Collection Tubes | 100 |
| 2ml Bead Tubes | 50 |
| Protease K | 50 mg |
| Protease Dissolve Buffer | 5 ml |
| DNase I (Powder) | 10 mg |
| DNase Buffer | 6 ml |
| Buffer CLB | 100 ml |
| Buffer SDS | 5 ml |
| Reagent DX | 1.5 ml |
| Buffer ACL | 30 ml |
| Buffer VHB* | 22 ml |
| Buffer RW2* | 20 ml |
| Buffer AVE | 15 ml |
Storage and Stability
Proteinase K and DNase I (Powder) should be stored at 2–8°C upon arrival. However, short-term storage (DNase I up to 1 week, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affecttheir performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
This kit is used for extracting total pathongen nucleic acid from low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate, culture, etc. The purified DNA/RNA is ready for downstream clinical in vitro detection such as Real Time PCR, biochip analysis, NGS and other related detection.
Usages:
For cultivating candida albicans.
Principle:
Corn flour extract provide carbon and other nutrients; polysorbate 80 as neutralizer; agar as medium coagulant.
Formulation(per liter):
Corn extract 6g
Polysorbate 80 10ml
Agar 15g
Final pH 5.6 ± 0.2
How to use:
1.Suspend 21g and 10ml of Tween 80 in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
500g
