Propargyl-PEG5-CH2CO2tBu enables the formation of triazole linkages with azide compounds or biomolecules in copper catalyzed Click Chemistry reactions. The t-butyl group can be hydrolyzed under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Propargyl-PEG5-CH2CO2tBu enables the formation of triazole linkages with azide compounds or biomolecules in copper catalyzed Click Chemistry reactions. The t-butyl group can be hydrolyzed under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
For the cultivation of E. coli in fermentation and molecular genetic studies.
Principle and Interpretation
Peptone provides nitrogen . Vitamins (including B vitamins) and certain trace elements are provided by yeast extract. Glucose provides carbon.Sodium ions for transport and osmotic balance are provided by sodium chloride. Agar is the solidifying agent.
Formulation
Ingredients
/liter
Peptone
10.0g
Yeast extract
5.0g
Sodium chloride
5.0g
Glucose
1.0g
Agar
15.0g
pH7.0±0.2 at 25°C
Preparation
Suspend 36g in 1 litre of distilled water. Bring to a boil to dissolve completely. Sterilize by autoclaving at 121°C for 15minutes.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 24 hours
Quality control strains
Growth
Escherichia coli ATCC25922
good
Storage and Shelf Life
2-30℃,Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
The Milenia swab PCR-System allows the direct detection of obligate spoilers from a swab. Areas of poor Hygiene and hotspots of Biofilm Formation can be quickly identified and monitored.
Brief Instructions
Storage
Components
Document
Name of Product Swab Detection Buffer Catalog Number MGSDB Short Info Extractionbuffer for swabs
Method/Platform Buffer for lateral flow assay Range/Assay Sensivity Test Principle The Milenia swab PCR-System allows the direct detection of obligate spoilers from a swab. Areas of poor Hygiene and hotspots of Biofilm Formation can be quickly identified and monitored.