Propargyl-PEG5-CH2CO2tBu

Description

For rapid, sensitive and accurate screening of potential Tyrosinase inhibitors

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Tyrosinase (EC 1.14.18.1) is a copper-binding enzyme that is expressed across a vast range of species ranging from bacteria and fungi to mammals. It is involved in two sequential reactions of the melanin synthesis pathway: first being the hydroxylation of a monophenol and second the conversion of an ortho-diphenol to a quinone. Quinone then undergoes a series of reactions including polymerization to form melanin.

Tyrosinase is of great interest to the agriculture industry since it causes browning of fruits, vegetable, and mushrooms, as well as to the cosmetic industry as it causes skin darkening. Development and screening of tyrosinase inhibitors, therefore, is very useful for conditions such as hyperpigmentation and melasma. Tyrosinase activity is significantly increased in melanoma. Therefore, the detection of tyrosinase activity could be promising as a specific diagnostic test for melanoma and may be useful in monitoring patient response to melanoma treatments.

This Tyrosinase Activity Assay Kit is a simple one-step, plate-based assay for the measurement of tyrosinase activity in biological samples. In this assay, tyrosinase catalyzes the conversion of a phenolic substrate to a Quinone intermediate, which reacts with the tyrosine enhancer forming a highly stable chromophore with absorbance at 520 nm. The assay can detect as low as 30 μU Tyrosinase in biological samples.

Highly reproducibility
Highly Sensitive Assay to Screen for Tyrosinase Activity
Stable formulation of ready to use Reaction Facilitator (tyrosinase)

The kit was developed for construction of high quality libraries for next generation sequencing (illumina and MGI Platforms). The kit needs double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library preparation, and is compatible with DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.). Library multiplexing is possible with different types of indexes.

The kit was optimized for next generation sequencing (NGS) library preparation with different types of samples. Most of DNA library preparation requires the ligation of sheared DNA fragments to library adaptors and the DNA library preparation is closely related to the quality of NGS data. With BioDynami’s unique DNA library preparation technologies, the fast and simple kit allows high quality NGS library preparation to be completed in 1.5 hours with only 10 minutes of hands-on time.

<img decoding="async" src="https://biodynami.com/wp-content/uploads/2020/07/NGS-DNA-Library-Prep-Kit-illumina-1.png" alt=""

Some genomic regions are very difficult to be covered evenly and usually result in very low coverage rate or gap in these regions.

Typical difficult regions are:
• with high GC contents
• have secondary structures: mainly due to repeat sequences
• the worst cases: have both high GC contents and repeated sequences.

Example: human TERT gene is one of the most difficult regions as shown above. NGS data showed that BioDynami kit has the best performance to cover the extremely difficult human TERT gene region.

Limited GC bias across whole genome

<img decoding="async" src="https://biodynami.com/wp-content/uploads/2020/07/BioDynami-NGS-DNA-Library-Prep-Kit-GC-bias.png" alt="

Three index types are available for the illumina platform kits:

Non-index (illumina Cat.# 30009): Libraries do not have index.

Index (illumina Cat.# 30021): A unique barcode sequence with 6 bases has been included in each of the index primers. RNA Sequencing library multiplexing is possible with up to 48 samples. Index information can be downloaded here.

Unique dual index (illumina Cat.# 30023): RNA Sequencing library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. the unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors. Index information can be downloaded here.

Indexes are available for the MGI platform kits (Cat.# 34021).

The kit was developed for construction of high quality libraries for next generation sequencing (illumina and MGI Platforms). The kit needs double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library preparation, and is compatible with DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.). Library multiplexing is possible with different types of indexes.

For simplified collection and shipment of leaf samples for our premium DNA Extraction and Genotyping Lab Services.

About

3CR Bio’s Leaf Sample Collection Kit – Your Gateway to High-Quality DNA Extraction and Genotyping Lab Services

The correct sampling and storage of leaf samples for DNA extraction have a significant impact on the final quality and quantity of the DNA extracted. Our simple, lightweight kits provide everything you need to sample and ship your leaf tissue from anywhere in the world, so it reaches us in top condition.

Use the 3CR Bioscience Leaf Sample Collection Kit to sample and prepare leaf tissue optimally when shipping to us for DNA extraction and Genotyping. Full instructions available in the Leaf Sample Collection User Guide.

Kit Contents

• 1 x 96-tube storage rack containing individual 2D-barcoded tubes sealed with a secure locking lid.
• 1 x Sponge pad to fit inside the lid of the storage rack for shipping.
• 1 x Sealed black plastic packet containing 2 x 10g sachets of desiccant.
• 1 x Large clear sealable plastic bag.
• 1 x Basic leaf cutting tool and leaf cutting mat (order separately)

or

• 1 x Advanced cutting tool (order separately)
• Plate Map for recording sample IDs (image not shown, provided in paper and electronic form)

The Plant Kit is used in combination with our Lab Services.
Visit the Lab Services page for more information about our DNA Extraction, Endpoint Genotyping, and GBTS services.

Here you can also:

• View an interactive workflow infographic detailing our user-friendly ordering, shipping, processing, and data retrieval.
• Access our online customer portal to register, submit projects. and review data.
• Request a quick quote

Seamless, reliable, and very competitive on price. We look forward to working with you.

For simplified collection and shipment of leaf samples for our premium DNA Extraction and Genotyping Lab Services.