Propargyl-PEG5-Ms

TCO-PEG24-DBCO is a monodisperse, long PEG linker featuring a trans-cyclooctene and a DBCO group. DBCO easily reacts with azides through click chemistry, while the TCO group readily reacts with tetrazine-containing compounds.

TCO-PEG24-DBCO is a monodisperse, long PEG linker featuring a trans-cyclooctene and a DBCO group. DBCO easily reacts with azides through click chemistry, while the TCO group readily reacts with tetrazine-containing compounds.

Description

The ExcelTaq™ Hot Start II DNA Polymerase is a mixture of an aptamer-based inhibitor and a recombinant thermo-stable Taq DNA polymerase designed for preventing or minimizing non-specific DNA amplification in PCR reaction. The inactivation of polymerase is achieved by a reversible binding of the aptamer to the polymerase at temperatures below 45°C. The aptamer inhibitor releases polymerase during normal PCR cycling. The aptamer-based inhibition omits the time-consuming initial activation step required by chemically modified or antibody-based hot start polymerases.

The high specificity and sensitivity of ExcelTaq™ Hot Start II DNA Polymerase allows sensitive detection from limited amount of DNA templates, such as 1 pg of cDNA or 1 fg of plasmid DNA. With a high DNA synthesis rate and high thermo-stability, the ExcelTaq™ Hot Start II DNA Polymerase allows reactions to be set up at room temperature and is suitable for common and specialized PCR applications

Features

• Aptamer-based hot start PCR
• Reversible enzyme inactivation
• Omits extra enzyme activation step
• Convenient for room temperature PCR set-up
• High yield and specificity of target amplicons
• Wide range of amplicon length (up to 10 kb)
• High sensitivity (as low as 1 fg of plasmid)

Applications 

• High specificity PCR
• Generation of PCR products for TA cloning
• Routine PCR, multiplex PCR, colony PCR, and RT-PCR

Storage

-20°C for 24 months 

The ExcelTaq™ Hot Start II DNA Polymerase is a mixture of an aptamer-based inhibitor and a recombinant thermo-stable Taq DNA polymerase designed for preventing or minimizing non-specific DNA amplification in PCR reaction. The inactivation of polymerase is achieved by a reversible binding of the aptamer to the polymerase at temperatures below 45°C. The aptamer inhibitor releases polymerase during normal PCR cycling. The aptamer-based inhibition omits the time-consuming initial activation step required by chemically modified or antibody-based hot start polymerases.

The high specificity and sensitivity of ExcelTaq™ Hot Start II DNA Polymerase allows sensitive detection from limited amount of DNA templates, such as 1 pg of cDNA or 1 fg of plasmid DNA. With a high DNA synthesis rate and high thermo-stability, the ExcelTaq™ Hot Start II DNA Polymerase allows reactions to be set up at room temperature and is suitable for common and specialized PCR applications

Overview

• Reliable and cost-effective
• Non-invasive, user-friendly, convenient sample collection & preservation in a single tube system
• Preserved exosomes are stable for 2 years at ambient temperature
• Shipping accessories can be purchased separately
• Compatible with Norgen’s Saliva Exosome Purification Kit (Cat. 65300)
• RNA can be isolated from the purified exosomes using Norgen’s Exosome RNA Purification kit (Cat. 58000)

Norgen’s Saliva Exosome Collection and Preservation Kit is an all-in-one solution designed for 1) simple and non-invasive saliva collection; 2) preservation of exosomes in saliva samples at ambient temperature; and 3) isolation of high quality intact exosomes and exosomal RNA within a laboratory setting. The Saliva Exosome Collection, Preservation and Isolation Kit contains 50 Individual Saliva Exosome Collection and Preservation Devices. Each of the 50 individual Saliva Exosome Collection and Preservation Devices consists of 3 components:

• Saliva Collection Funnel and Collection Tube that contains Norgen’s Preservative in a dried format
• Collection Tube Cap
• ID Label

Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed, the Collection Tube is securely capped with Collection Tube Cap. The collected saliva is mixed by gentle inversion for several times till the saliva colour changes into yellow. The Saliva Collection Tube is subsequently sent to the laboratory for the purification of intact exosomes and for the subsequent isolation and analysis of saliva exosomal RNA. The saliva exosomes in preserved samples are stable for 2 years at room temperature.

Details

Shelf Life and Handling

• The Collection Device should be kept tightly sealed and stored at room temperature for up to 2 years without any reduction in kit performance (The collection due date is written on the device label).
• The Collection Tube, the Collection Funnel and the Device Container of each Individual Saliva DNA Collection and Preservation Device are recyclable.