Introduction

Usages:
Peptone obtained by enzymatic digestion of selected animal tissues, widely used in the preparation of microbiological culture media.

Technical specification:
Total nitrogen(TN)—————————≥14.5%
Amino nitrogen(AN) ————————≥2.0%
Moisture—————————————≤5.0%
Ash———————————————≤5.0%
Clˉ——————————————–≤3.0%
PH(2% solution)——————————5-7

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.

Specifications: 500g/bottle; 10kg /bag

500g/bottle; 10kg /bag

Introduction

This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 50mg simple plant using 96 well bind plate
Applications	PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method	96 well plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Economic plant samples
Sample amount	Fresh / frozen plant samples: ≤50mgDried plant / seed samples: ≤15mg
Elution volume	≥75μl
Time per run	30-50 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.

Advantages

• High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, blot hybridization, etc.
• High throughput – 96 samples can be processed simultaneously by 96 well plate
• High permeability – using composite membrane technology, no hole plugging

Kit Contents

Contents	D316702	D316703
Purification Times	4 x 96 Preps	20 x 96 Preps
RNase Solution	5 ml	22 ml
Elution Buffer	120 ml	500 ml
Buffer SPL	200 ml	2 x 500 ml
Buffer PS	100 ml	400 ml
Buffer GW1	220 ml	5 x 220 ml
Buffer GW2	100 ml	3 x 100 ml
1.6ml Collection Plate	4	20
HiPure gDNA Plate	4	20
2.2ml Collection Plate(DW Plate)	8	40
0.8ml Collection Plate(DW Plate)	4	20
Sealing Film	20	100

Storage and Stability

RNase solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Name	CAT NO	Fresh / frozen tissue amount	Dried tissue amount	Column type	Elution volume	Yield (muscle)
HiPure Plant DNA Mini Kit	D3187	150mg	40mg	1.5ml column	50 – 100μl	3-70μg
HiPure HP Plant DNA Maxi Kit	D3163	5g	1g	50ml column	0.7 – 3ml	75-1250μg
HiPure SF Plant DNA Kit	D3164	100mg	20mg	1.5ml column	50 – 100μl	3-50μg
HiPure SF Plant DNA 96 Kit	D3167	50mg	15mg	96 well plate	75 – 150μl	3-30μg

This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.

K-FRUC

SKU: 700004285

100 assays per kit

Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Fructan
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	410
Signal Response:	Increase
Linear Range:	2.3 to 55 µg of D-fructose or D-glucose per assay
Limit of Detection:	0.16 g/100 g
Total Assay Time:	~ 90 min
Application examples:	Flours, infant formula, animal feed, pet foods, plant materials (e.g. onion), food products and other materials
Method recognition:	AACC Method 32-32.01, AOAC Method 999.03, AOAC Method 2016.14, AOAC Method 2018.07 and CODEX Method Type III

The Fructan Assay Kit is suitable for the specific measurement of fructan in plant extracts, animal feed and food products containing starch, sucrose and other sugars. It is used in three validated methods for the determination of fructan: AOAC method 999.03 (foods), AOAC method 2018.07 (Animal Feed) and AOAC method 2016.14 (infant formula and adult nutritionals).

New, improved procedure.

In the most recent development, a recombinant endo-levanase has been incorporated into the fructanase mixture, extending the use of the method to the measurement of levan-type fructans as are present in grasses such as timothy, cocksfoot, ryegrass and red fescue.

The method described in this booklet employs ultra-pure, recombinant enzymes and specifically measures fructans including inulin-type fructans from chicory, dahlia, jerusalem artichoke; highly branched fructans from onion and wheat stems and leaves; and levan-type fructans from pasture grasses such as timothy grass. The enzymes employed are completely devoid of contaminating enzymes active on β-glucan or gluco-oligosaccharides.

Browse our full range of polysaccharide assay kits.

Validation of Methods

Advantages

• Very cost effective 
• All kit reagents stable for > 2 years after preparation 
• Unaffected by high sucrose / reducing sugar concentrations  
• Fructan kits are only available from Megazyme 
• Simple format 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The Fructan Assay Kit is suitable for the specific measurement of fructan in plant extracts, animal feed and food products containing starch, sucrose and other sugars. It is used in three validated methods for the determination of fructan: AOAC method 999.03 (foods), AOAC method 2018.07 (Animal Feed) and AOAC method 2016.14 (infant formula and adult nutritionals).