Propargyl-PEG6-amine

The NGS DNA Fragmentation & Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality libraries for next generation sequencing. The kit uses intact genomic DNA (EDTA-free DNA or DNA resuspended in TE buffer) as input DNA without an additional DNA fragmentation step. Our technology provides a fast and simple workflow. DNA libraries can be generated around 2 hours with only 10 min hands-on time. Library multiplexing is possible.

NGS DNA Fragmentation & Library Prep Kit Workflow

The incorporation of DNA fragmentation in the kit makes it possible to directly use intact genomic DNA as input DNA without the need of mechanical DNA shearing or enzymatic DNA fragmentation. The NGS DNA Fragmentation & Library Prep Kit does not generate sequencing bias as compared to library using mechanical sheared DNA as input. Sequence coverage is also consistent between enzymatic shearing and mechanical shearing. The library size is inversely correlated with the incubation time of step 1 at 20°C.

Three index types are available for the kit of the illumina platform:

Non-index (Cat.# 30026): Libraries do not have index.

Index (Cat.# 30028): Each of the index primers contains a unique index sequence of 6 bases. Library multiplexing for 48 samples is possible. Index information can be downloaded here.

Unique dual index (Cat.# 30030): Library multiplexing for 96 samples is possible. With the unique features of our 4-Base Difference Index System, the index sequence is 8-base long and each index has 4 bases different from others. Our unique dual index primers effectively identify sequencing errors such as index hopping, mis-assignment of reads, and de-multiplexing errors etc. The unique dual index primers set consists of 96 pre-mixed unique pairs of  index primers. Index information can be downloaded here.

Indexes are available for the MGI platform kits (Cat.# 34028).

Kit features:

• • • 1.5-hour protocol from intact genomic DNA to NGS library
    • Intact genomic DNA as input, DNA fragmentation is not needed.
    • Works with both EDTA-free DNA and DNA resuspended in TE buffer
    • Simple workflow: Less steps
    • Guaranteed quality: Higher library conversion efficiency

Library conversion efficiency: 100 ng, 300 ng and 500 ng of intact genomic DNA were used as input.

The library size is inversely correlated with the incubation time of step 1 at 20°C.

NGS data comparison: enzymatic shearing versus mechanical shearing

Enzymatic shearing
• DNA shearing and library prep: BioDynami NGS DNA Fragmentation & Library Prep Kit
Mechanical shearing
• DNA shearing: Covaris sonication
• Library prep: BioDynami NGS DNA Library Prep Kit.

The NGS DNA Fragmentation & Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality libraries for next generation sequencing. The kit uses intact genomic DNA (EDTA-free DNA or DNA resuspended in TE buffer) as input DNA without an additional DNA fragmentation step. Our technology provides a fast and simple workflow. DNA libraries can be generated around 2 hours with only 10 min hands-on time. Library multiplexing is possible.

Application 

Hereby we (Changsha Yingtai Instrument Co.,Ltd) confirm that CDL7M centrifuge is recommended for Graphene Base Manufacturing Industries. This centrifuge type is widely used in Large Laboratory Systems, Graphene Base Manufacturing Industries, and also used in the field of blood stations, pharmaceutical factories, biochemistry, biological products, and so on

 Features 

1. Widely used in the field of blood bank, pharmaceutical factory and laboratory.

2. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.

3. Digital display which indicates the program, speed, time,RCF, temperature.

4. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.

5. There are 3 kinds of rotors for your choice.

6. Electric lid lock, compact design, super speed and imbalance protection.

7. The centrifuge body is made of high-quality steel, safe and reliable.

 CDL7M Technical Parameter：

Max. Speed	8000rpm
Max. RCF	14551×g
Max. Capacity	6×2400ml
Time Range	1~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 65
Temperature	-20~40℃
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V/380V  50HZ/60HZ
Size (W x D x Hmm)	940×890×1000mm
Net Weight(Kg)	570KG
Certificates	CE,ISO & Calibration report are available

 Matched Rotors for CDL7M 

Order No.	Rotor No.	Max Speed (rpm)	Max Volume(ml)	Max.RCF（×g）
7M-1	Swing Rotor	4000	6×2400ml	5330
7M-2	Angle Rotor	8000	6×1000ml	14551
7M-3	Swing Rotor	4200	2×6×1000ml	5680

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Ten discrete fragments ranging from 300 bp to 5000 bp

The Norgen MidRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our MidRanger contains ten discrete fragments ranging from 300 bp to 5000 bp. This Ladder is ideal for sizing larger PCR products (>1kb) and most cloning applications.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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MidRanger 1kb DNA Ladder (Cat# 11700) – 100 loads

Ladder Properties:
• Ten discrete fragments ranging from 300 bp to 5000 bp

Fragment	Size (bp)	Mass (ng)
1	5000	88
2	4000	78
3	3000	67
4	2500	58
5	2000	50
6	1500	43
7	1000	25
8	700	34
9	500	33
10	300	24

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.