Propargyl-PEG6-Ms is a PEG reagent that enables Click Chemistry reactions with azide-bearing compounds or biomolecules; copper is needed as a catalyst. The PEG spacer increases the water solubility of the reagent in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Propargyl-PEG6-Ms is a PEG reagent that enables Click Chemistry reactions with azide-bearing compounds or biomolecules; copper is needed as a catalyst. The PEG spacer increases the water solubility of the reagent in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
2,5-Dioxopyrrolidin-1-yl 2-(prop-2-yn-1-yloxy)acetate is a Click Chemistry reagent with a propargyl group and an NHS ester group. The propargyl group can react with biomolecules containing azide group via copper catalyzed Click Chemistry reaction. The NHS ester is an amine reactive group which can be used for derivatizing peptides, antibodies, amine coated surfaces, etc. Reagent grade, for research use only.
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2,5-Dioxopyrrolidin-1-yl 2-(prop-2-yn-1-yloxy)acetate is a Click Chemistry reagent with a propargyl group and an NHS ester group. The propargyl group can react with biomolecules containing azide group via copper catalyzed Click Chemistry reaction. The NHS ester is an amine reactive group which can be used for derivatizing peptides, antibodies, amine coated surfaces, etc. Reagent grade, for research use only.
MagPure Seed DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and seed. This kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo. Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridizationand transgenosis detection.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 50-100 mg easy-grinded plant (tender leaf) and seed
Applications
PCR, transgene detection, fluorescence quantitative PCR, southern blot, SNP site analysis, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Conventional economic plant samples
Sample amount
50-100mg
Elution volume
≥50μl
Time per run
≤60 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
Safe – require no phenol or chloroform extraction
Fast – several samples can be extracted in 60 minutes
High purity – high quality DNA, completely remove inhibitors
Kit Contents
Contents
D635201
D635202
D635203
Purification Times
48 Preps
96 Preps
5 x 96 Preps
MagPure Particles
1.7 ml
4 ml
18 ml
Buffer SOL
40 ml
90 ml
500 ml
Buffer SDS
4 ml
9 ml
50 ml
Buffer MPB
40 ml
70 ml
350 ml
Buffer GW1
26 ml
53 ml
220 ml
Elution Buffer
10 ml
30 ml
120 ml
Storage and Stability
MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
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MagPure Seed DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and seed. This kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo. Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridizationand transgenosis detection.
The genesig® Dengue, Zika and Chikungunya Virus Multiplex kit is designed for the detection and differentiation of Dengue virus, Zika virus (ZIKV) and Chikungunya virus (CHIKV) only. Individual tests have been designed in the conserved regions of each virus such that all isolates and subtypes will be detected simultaneously in the same test. The Dengue component of the test will detect subtypes 1, 2, 3 and 4 but will not differentiate between them. A positive Dengue test results indicates that the sample has either one of these four subtypes.
The primers and probe sequences in this kit have 100% homology with a broad range of clinically relevant reference sequences based on a comprehensive bioinformatics analysis. They therefore have a very broad quantification profile.
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Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions