Propargyl-PEG6-Ms is a PEG reagent that enables Click Chemistry reactions with azide-bearing compounds or biomolecules; copper is needed as a catalyst. The PEG spacer increases the water solubility of the reagent in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG6-Ms is a PEG reagent that enables Click Chemistry reactions with azide-bearing compounds or biomolecules; copper is needed as a catalyst. The PEG spacer increases the water solubility of the reagent in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product provides fast and easy method for purification of high purity DNA from bone samples. The obtained DNA can be directly used for PCR and STR detection down stream application.
| Contents | D312402 | D312403 |
| Purification Times | 50 | 250 |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2ml Collection Tubes | 50 | 250 |
| Buffer BGL | 40 ml | 180 ml |
| Buffer GXP | 30 ml | 150 ml |
| Buffer GW1* | 13 ml | 53 ml |
| Buffer GW2* | 20 ml | 2 x 50 ml |
| DTT Powder | 235 mg | 2 x 235 mg |
| Proteinase K | 48 mg | 240 mg |
| Protease Dissolve Buffer | 5 ml | 15 ml |
| Elution Buffer | 15 ml | 60 ml |
Storage and Stability
This product can be stored at room temperature (15~25°C) for 18 months. Proteinase K/DTT dry powder can be transported and stored at room temperature. For long-term storage (>6 months), it is recommended to store at -20~8°C. Dissolved Proteinase K should be stored at -20~8°C. The dissolved DTT should be stored at -20°C.
This product provides fast and easy method for purification of high purity DNA from bone samples. The obtained DNA can be directly used for PCR and STR detection down stream application.
K-PullG6
SKU: 700004329
100/200 assays per kit
| Content: | 100 / 200 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Pullulanase/Limit-Dextrinase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Signal Response: | Increase |
| Limit of Detection: | 0.18 U/mL for pullulanase preparations (50-fold dilution) 0.01 U/g for limit dextrinase in milled malt |
| Reproducibility (%): | ~ 3% |
| Total Assay Time: | ~ 10 min (Pullanase), ~ 30 min (Limit-Dextrinase) |
| Application examples: | Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts. |
| Method recognition: | Novel method |
PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-63-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.
Explore more of our assay kit products for enzyme activity measurement.
Advantages
The Opentrons Flex Waste Chute GEN1 occupies position D3 and is used to accept waste such as tips, tip racks, and plates in high volumes. The gripper will pick up the consumable and dispose it into the chute based on your workflow needs. The waste chute is neccesary for all high-throughput workflows and can be paired with the Opentrons Flex Deck Expansion Set to maximize the throughput capabilities of the Opentrons Flex. The following consumables can disposed of into the waste chute:
– NEST 2 mL Deep Well Plate, V Bottom
– Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt
– NEST 96 well Plate Flat
– All Opentrons Flex tip racks
The Opentrons Flex Waste Chute GEN1 occupies position D3 and is used to accept waste such as tips, tip racks, and plates in high volumes. The gripper will pick up the consumable and dispose it into the chute based on your workflow needs. The waste chute is neccesary for all high-throughput workflows and can be paired with the Opentrons Flex Deck Expansion Set to maximize the throughput capabilities of the Opentrons Flex. The following consumables can disposed of into the waste chute:
– NEST 2 mL Deep Well Plate, V Bottom
– Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt
– NEST 96 well Plate Flat
– All Opentrons Flex tip racks
