Propargyl-PEG6-Ms

RNA into DNA and PCR in one step? Then, this enzyme will simplify PCR analysis from RNA templates reducing labor and time. RT-KTQ2 was evolved from the thermostable KlenTaq DNA polymerase with no significant reverse transcriptase activity. Four mutations ensure that the variant is reverse transcriptase active and even PCR active, while maintaining the thermostability. This allows to perform reactions at high temperatures minimizing problems encountered with strong secondary structures in RNA that melt at elevated temperatures. For further information refer to the original publication.

Available upon request and for R&D use only – Contact Us

RT-KTQ2 DNA polymerase is supplied as a 5 µM solution containing glycerol and is supplied together with 10x reaction buffer.

The enzyme can also be used for real-time cycling, when adding a suitable dye.

RNA into DNA and PCR in one step? Then, this enzyme will simplify PCR analysis from RNA templates reducing labor and time. RT-KTQ2 was evolved from the thermostable KlenTaq DNA polymerase with no significant reverse transcriptase activity. Four mutations ensure that the variant is reverse transcriptase active and even PCR active, while maintaining the thermostability. This allows to perform reactions at high temperatures minimizing problems encountered with strong secondary structures in RNA that melt at elevated temperatures. For further information refer to the original publication.

Description 

The FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) is designed to substitute the common Coomassie Blue protein staining method, offering greater sensitivity and ease of operation. Unlike Coomassie Blue stain, the FluoroStain Protein Fluorescent Staining Dye binds to protein with high specificity, making destaining process an option rather than a requirement. With further reduction of background signals via destaining process, the FluoroStain™ is capable of achieving detection level parallel to silver staining without specialized imaging equipment, making it one of the most sensitive dyes available. In addition to its remarkable sensitivity, the FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) brings a more reliable and safer user experience, since the stained gel can be visualized with blue-light illumination, avoiding the risk of skin/ eye damage caused by UV light. For best result, we suggest using B-BOX™ Blue Light LED epi-illuminator to visualize and analyze the gel stained with FluoroStain Protein Fluorescent Staining Dye (Red, 1000×). The FluoroStain™ Protein Fluorescent Staining Dye is compatible to the analysis of mass spectra, i.e. LC-MS/MS, MALDI-TOF, etc.

Spectral Characteristics

When it is bound with bovine serum albumin (BSA), the fluorescent emission of FluoroStain Protein Fluorescent Staining Dye can be excited by UV and blue light sources, with excitation peaks around 369 and 517 nm and emission at 605 nm. In absence of BSA, FluoroStain Protein Fluorescent Staining Dye shows ignorable fluorescence as compared with protein-bound form, therefore giving a clear background for photographic analysis.

These spectral characteristics made this fluorescent dye compatible with a wide variety of gel reading facilities, including UV/ blue light epi- and transilluminator, argon laser and mercury-arc lamp excitation gel scanners.

Storage 

Protected from light 
-20°C for 24 months 

The FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) is designed to substitute the common Coomassie Blue protein staining method, offering greater sensitivity and ease of operation. Unlike Coomassie Blue stain, the FluoroStain Protein Fluorescent Staining Dye binds to protein with high specificity, making destaining process an option rather than a requirement. With further reduction of background signals via destaining process, the FluoroStain™ is capable of achieving detection level parallel to silver staining without specialized imaging equipment, making it one of the most sensitive dyes available. In addition to its remarkable sensitivity, the FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) brings a more reliable and safer user experience, since the stained gel can be visualized with blue-light illumination, avoiding the risk of skin/ eye damage caused by UV light. For best result, we suggest using B-BOX™ Blue Light LED epi-illuminator to visualize and analyze the gel stained with FluoroStain Protein Fluorescent Staining Dye (Red, 1000×). The FluoroStain™ Protein Fluorescent Staining Dye is compatible to the analysis of mass spectra, i.e. LC-MS/MS, MALDI-TOF, etc.

DBCO-PEG10-DBCO is a monodisperse click chemistry linker containing two terminal DBCO groups with hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG10-DBCO is a monodisperse click chemistry linker containing two terminal DBCO groups with hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.