Introduction

Intended Use

For the selective isolation and culture of Listeria monocytogenes.

Principle and Interpretation

Peptone provides the carbon and nitrogen sources necessary for growth; yeast extract powder and starch provide carbon and nitrogen sources, vitamins and growth factors; sodium chloride can maintain a balanced osmotic pressure; glucose provides a carbon source; Listeria hydrolyzes esculin and reacts with iron ions to form black 6,7-dihydroxycoumarin; mannitol is a fermentable sugar, and phenol red is a pH indicator; lithium chloride and other antibiotics can inhibit Gram-negative bacteria and most Gram-positive bacteria; agar is the coagulant of the culture medium.

Formulation

Ingredients	/liter
Peptone	23 g
Starch	1 g
NaCl	5 g
Columbia agar	13 g
Mannitol	10 g
Ferric ammonium citrate	0.5 g
Esculin (aesculin)	0.8 g
Dextrose (glucose)	0.5 g
Lithium chloride	15.0 g
Phenol red	0.08 g
pH7.2±0.2 at 25°C

Preparation

Weigh 68.9g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, divide into Erlenmeyer bottles, sterilize at 121℃ for 15min, cool to room temperature and set aside. Heat to dissolve agar before use, cool to 50℃, add 1 tube of supporting reagent (SR0140) per 100mL of basal culture medium, shake well and pour into sterilized culture dish.

Quality Control

The following quality control strains were inoculated and cultured at 35-37℃ for 24h. The results are as follows:

Quality control strains	Growth
Listeria monocytogenes ATCC19115	Gray-green colonies with a black depression in the center and black surrounding
Enterococcus faecalis ATCC29212	–
Escherichia coli ATCC25922	–

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Intended Use For the selective isolation and culture of Listeria monocytogenes. Principle and Interpretation Peptone provides the carbon and nitrogen sources necessary for growth; yeast ex……

Overview

• All shipping requirements in one convenient mailer
• Leak-proof biohazard bags with adsorbent pads for enhanced safety
• Matches the standards of OSHA as well as the IATA regulations

Norgen’s Shipping Accessories provides a safe and convenient tool for shipping non-infectious biological specimens. The accessories are contained in individual SHIP TO envelopes that are used for shipping of both the accessories as well as the individual sample collection device to the donor/user.

Each SHIP TO envelope contains:

1. A Biohazard Specimen Bag with absorbent pad;
2. A Bubble Envelope labeled as “Exempt Human Specimen”;
3. 2 blank labels; 
4. Shipping Instructions

The Biohazard Specimen Bag is a sure-seal zipper bag to hold the collected specimen and protect against accidental spills. The bag contains an absorbent pad that can adsorb up to 4 mL of liquid and meets current labeling requirements of OSHA standard 29CFR1910.1030 regarding biohazard symbols. The Bubble Envelope is used as a RETURN envelope for the donor/user to ship the collected sample back to the lab for analysis. The user will first place the collected sample into the Biohazard Specimen Bag, and then place the Biohazard Specimen Bag into the Bubble Envelope. The Bubble Envelope protects against moisture and punctures, and facilitates smooth insertion with convenient self-sealing closure. The 2 labels are used for both the outer SHIP TO envelope and the RETURN bubble envelope. The SHIP TO label will be filled out to contain the mailing information of the donor and the RETURN label will be filled out to contain the address to which the sample should be returned. The labels can be supplied as sheets together with the word template file for ease of printing. Norgen’s Shipping Accessories match the regulations of the International Air Transport Association (IATA).

Details

Shipping Accessories Contents
SHIP TO Envelopes	50
Product Insert	1
SHIP TO Envelope Contents
Biohazard Specimen Bag with absorbent pad	1
Bubble Envelope	1
Labels	2
Shipping Instructions	1

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 1-10μl blood, <10mg tissue, urine, blood stain, seminal stain
Applications	PCR, southern bolt and virus detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Animal tissues, blood stain, urine, seminal stain and various forensic samples
Sample amount	Blood:1-100μl, Tissue:<10mg
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).

Advantages

• Fast – several samples can be extracted in 20 minutes (after digestion)
• High purity – purified DNA can be directly used in various downstream applications
• High recovery – DNA can be recovered at the level of PG
• Good repeatability – silica technology can obtain ideal results every time

Kit Contents

Contents	D312502	D312503
Purification Times	50 Preps	250 Preps
Buffer ATL	15 ml	60 ml
Buffer AL	15 ml	60 ml
Buffer GW1*	22 ml	66 ml
Buffer GW2*	20 ml	2 x 50 ml
Carrier RNA	310 μg	2 x 310 µg
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Buffer AE	15 ml	60 ml
HiPure DNA Mini Columns I	50	2 x 125
2 ml Collection Tubes	100	5 x 100

Storage and Stability

Carrier RNA and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.

Experiment Data

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.