Propargyl-PEG7-alcohol is a propargyl linker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG7-alcohol is a propargyl linker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG7-alcohol is a propargyl linker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
Specifications
| Features | Specifications |
| Concentration | 70 mg/ml |
| Appearance | Suspension of yellowish brown particles |
| Surface functional group | Si-OH, Silanol |
| Dispersibility | Polydisperse Amorphous |
| Particle size | 0.2-2 μm |
| Preservation conditions | Room Temperature, valid for up to 2 years.It is recommended to store in 2-8°C to prevent microbial growth. |
| Magnetic response speed | ~60 seconds |
| Settling velocity | >10 minutes |
| High salt mediated binding | >2M guanidine isothiocyanate, DNA recovery up to 80% |
| Alcohol mediated binding | 2M guanidine hydrochloride / isopropanol (30%), and the recovery of DNA / RNA was as high as 85% |
| PEG8000 mediated binding | The recovery of DNA/RNA was up to 85% |
| DNase/RNase | Not detected |
| DNA residue | <1 ppm |
| Recommended application | Plasmid extraction, gel DNA recovery, viral nucleic acid isolation |
Principle
Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.
Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.
After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.
Ordering information
| CAT.No. | Product Name | Package |
| C14110 | MagPure Particles N | 100 ml |
| C14111 | MagPure Particles N | 400 ml |
| C14112 | MagPure Particles N | 3 x 400 ml |
| C14113 | MagPure Particles N | 10 x 400 ml |
| Features | MagPure Particles | MagPure Particles N | MagPure Particles G | MagPure Particles F | MagBind Particles |
| Cat.No. | C1410 | C1411 | C1412 | C1414 | C1413 |
| Concentration | 100mg/ml | 70mg/ml | 40mg/ml | 50mg/ml | 10mg/ml |
| Form | Amorphous and Porous | Amorphous and Porous | Porous | Amorphous | Nonporous |
| Surface function | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | COOH, Carboxyl Beads |
| Dispersion | Polydisperse | Polydisperse | Monodisperse | Monodisperse | Monodisperse |
| Particle Size | 1.5-5μm | 0.2-2μm | 1-1.5μm | 0.2-1.5μm | 0.8-1μm |
| Color | Black | Yellowish Brown | Dark Brown | Dark Brown | Yellowish Brown |
| Magnetic response | 15-30s | ~60s | ~30s | 20s | 120s |
| Settling Time (1ml) | >5min | >10min | >3min | >3min | >2h |
| Usage (0.2ml Sample) | 20μl | 20μl | 20-30μl | 20-30μl | 20-30μl |
| DNA Recover Rate (only 4M GITC) | >80% | >80% | >80% | >80% | 0 |
| DNA Recover Rate (10% PEG8000/NaCl) | >85% | >85% | >85% | >85% | >90% |
| Recommended Use | gDNA/RNA Isolation from Blood, Tissue, Plant, Swab, Spots, Stool, Soil and etc.Viral DNA/RNA IsolationAgarose Gel DNA Purification | DNA/RNA Isolation from low nucleic acid content samplesPlasmid IsolationDNA/RNA Clean Up | Circulating DNA IsolationViral Nucleic acid IsolationgDNA Isolation FFPE DNA/RNA Isolation | Plasmid extractiongel DNA recoverygenomicDNA/RNA extraction viral nucleic acid extractionCirculating DNA extraction | DNA/RNA Clean Up and concentrationDNA/RNA Isolation from low nucleic acid content samplesResearch immuno assays |
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
Usages:
For cultivation of bifidobacteria and lactic acid metabolites test.
Principle:
Peptone, yeast extract provide nitrogen, vitamins, and growth factors; glucose as
fermentable sugars; cysteine – HCl, vitamin K1, hemin, magnesium sulfate, calcium
chloride, sodium chloride, sodium bicarbonate training offers various bifidobacteria growth factor; dipotassium hydrogenphosphate, potassium dihydrogenphosphate buffer.
Formulation ( per liter):
Peptone 20g
Glucose 5g
Yeast powder 10g
Cysteine – hydrochloride 0.5g
Calcium chloride 0.008g
Magnesium sulfate 0.008g
Dipotassium hydrogen phosphate 0.04g
Potassium dihydrogen phosphate 0.04g
Sodium bicarbonate 0.4g
Sodium chloride 0.08g
Final pH 6.0 ± 0.2
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
250g
A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments >100bp from agarose gel(<0.3g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA |
| Applications | PCR, NGS, chip analysis, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Agarose Gel, PCR products, enzymatic reaction solution |
| Sample amount | Agarose Gel: <0.3g, PCR products: ≤50µl |
| Recovery | 80% |
| Elution volume | ≥30μl |
| Time per run | ≤30 minutes |
The Kit method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments (>100bp) and larger to paramagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.
Advantages
Kit Contents
| Contents | D500101 | D500102 | D500103 |
| Purification Times | 50 Preps | 500 Preps | 5000 Preps |
| Buffer GDP | 30 ml | 250 ml | 3 x 900 ml |
| MagPure Particles | 1.6 ml | 15 ml | 3 x 50 ml |
| Buffer DW1 | 20 ml | 180 ml | 2 x 800 ml |
| Elution Buffer(10mmTris, pH8.5) | 10 ml | 60 ml | 500 ml |
Storage and Stability
MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.