
Propargyl-PEG7-alcohol is a propargyl linker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG7-alcohol is a propargyl linker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG7-alcohol is a propargyl linker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This kit is designed to rich and extract 100bp-500bp circulating DNA from 5 ml cell-free body fluids (such asplasma, serum), and remove fragments above 500bp. Machine reaction only takes 90 minutes. Magnetic-particle technology provides high-quality DNA that is suitable for direct use in downstream applications such as PCR and next generation sequencing.
Specifications
Features | Specifications |
Main Functions | Isolation circulating DNA from 5ml plasma, serum, body fluids |
Applications | qPCR, NGS, etc. |
Purification technology | Magnetic beads technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Serum, plasma |
Sample amount | 5ml |
Elution volume | ≥40μl |
Time per run | ≤50 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
Contents | 1292750 | 12927200 |
Purification Times | 50 | 200 |
MagPure Particles G | 20 ml | 80 ml |
MagBind Particles (selection particles) | 14 ml | 58 ml |
Selection Solution | 100 ml | 400 ml |
Proteinase K | 300 mg | 1.2 g |
Protease Dissolve Buffer | 25 ml | 100 ml |
Buffer SDS(20%) | 15 ml | 60 ml |
Buffer MLK | 300 ml | 3 x 450 ml |
Buffer BST1 | 225 ml | 2x 450 ml |
Buffer MKW1 | 225 ml | 2x 450 ml |
Buffer MW2* | 50 ml | 2x 100 ml |
Buffer AE | 10 ml | 30 ml |
Storage and Stability
MagPure Particles G, MagBind Particles and Proteinase K should bestored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at roomtemperature (15–25°C) and are stable for at least 18 months underthese conditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
This kit is designed to rich and extract 100bp-500bp circulating DNA from 5 ml cell-free body fluids (such asplasma, serum), and remove fragments above 500bp. Machine reaction only takes 90 minutes. Magnetic-particle technology provides high-quality DNA that is suitable for direct use in downstream applications such as PCR and next generation sequencing.
Usages:
For selective isolation of Gram-negative bacteria, especially for Shigella and Salmonella.
Principle:
Yeast extract powder provide nitrogen, vitamins, growth factors; sodium chloride to maintain osmotic equilibrium ferric ammonium citrate of iron salts to produce a black iron sulfide; agar as medium coagulant; phenol red as pH indicator.
Formulation(per liter):
Xylose 3.50g
L-Lysine 5.00g
Lactose 7.50g
Sucrose 7.50 g
Sodium Chloride 5.00 g
Yeast Extract 3.00g
Sodium Desoxycholate 2.50g
Sodium Thiosulphate 6.80g
Fe-Ammonium Citrate 0.80g
Phenol Red 0.08g
Agar 13.50
Final PH 7.4±0.2
How to use:
1.Suspend 55.2g in 1Lof distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Do not autoclave ,cool to 50℃ and pour into sterile petri dishes.
2.Dilluted and treated samples.
Quality control:
Quality control strains were inoculated ,and cultured at 36 ± 1 ℃ for 24h ,results show as follows:
strain name strain code growth feature
Arizona bacteria CMCC (B) 47001 good black colonies
Salmonella typhimurium CMCC (B) 50115 good black colonies
Streptococcus faecalis CMCC32223 prohibited —
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Specifications: 250g/bottle
250g
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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