Propargyl-PEG8-alcohol

Overview

• Extract total RNA (including microRNA) from FFPE samples
• No phenol extraction step
• Includes DNase for optional on-column DNA removal
• Isolated RNA is of the highest quality and integrity
• Isolate a diversity of RNA species
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s FFPE RNA Purification Kits provide a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kits provide conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. These kits are able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform.

FFPE RNA Purification Kit (Spin Column)

Maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.

FFPE RNA Purification 96-Well Kit (High Throughput)

Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Maximum loading volume of 400 μL per well, and a maximum binding capacity of 50 μg per well.

Details

Supporting Data

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Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	400 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material:	5 slices of < 20 µm thick paraffin  25 mg of unsectioned block

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.

Component	Cat. 25300 (50 preps)	Cat. 25400 (2 x 96 preps)
Digestion Buffer A	25 mL	2 x 25 mL
Buffer RL	30 mL	2 x 30 mL
Enzyme Incubation Buffer	6 mL	2 x 6 mL
Wash Solution A	38 mL	2 x 38 mL
Elution Solution A	6 mL	2 x 20 mL
Proteinase K	12 mg	2 x 20 mg
DNase I	1 vial	2 x 500μL
Micro Spin Columns	50	–
96-Well Incubation Plate	–	2
96-Well Plate	–	2
Adhesive Tape	–	8
Collection Tubes	50	–
96-Well Collection Plate	–	2
Elution Tubes (1.7 mL)	50	–
96-Well Elution Plate	–	2
Product Insert	1	1

Sulfo DBCO-TFP Ester is a water-soluble, amine-reactive labeling reagent that enables simple and efficient incorporation of Sulfo DBCO moiety onto amine-containing molecules. The hydrophilic, sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules, in many cases making them completely soluble in aqueous media. A short spacer arm adds minimal mass to modified molecules.

Sulfo DBCO-TFP Ester is a water-soluble, amine-reactive labeling reagent that enables simple and efficient incorporation of Sulfo DBCO moiety onto amine-containing molecules. The hydrophilic, sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules, in many cases making them completely soluble in aqueous media. A short spacer arm adds minimal mass to modified molecules.

TD4N Table Top 5000rpm Low Speed Laboratory Centrifuge

TD4N Features:

1. Microprocessor control, less noisily, it is widely used to qualitative analysis of blood serum, plasma and urea in the fields of hospital, blood center, laboratory and biochemistry.

2. Brushelss motor, free maintenance, no powder pollution, quick in speed up and down.

3. The flexible axle driven system which drive the rotor directly, smooth in operation and small vibration.

4. There are many rotors for your choice, suitable for different specifications meet customers’ different requirements of separation.

5. Micro-computer control system, digital display the RCF, time and speed.

6. Electric lid lock, compact design, super speed and imbalance protection.

TD4N Technical Parameter:

Max. Speed	5000rpm	Speed Accuracy	±20rpm
Max. Volume	6x100ml	Power Supply	AC110V/220V 50HZ/60HZ
Max. RCF	3460xg	Noise	≤55dBA
Timer	0~99min	Net Weight	18Kg
Dimension	540x370x280mm	Certifications	CE, ISO & Calibration report are available.
Warranty	1 Year

Matched Rotors for TD4N:

Order No.	Rotor Type	Max.Speed(rpm)	Max.Volume(ml)	Max.RCF(xg)
4N-1	Swing Rotor	5000	6x10ml	3460
4N-2	Angle Rotor	4000	30×7/5ml	2250
4N-3	Angle Rotor	4000	18×15/10ml	2250
4N-4	Angle Rotor	4000	24x10ml	2200
4N-5	Angle Rotor	4000	12×15/7/5ml	2150
4N-6	Angle Rotor	4000	12x20ml	2200
4N-7	Angle Rotor	5000	6x15ml	2540
4N-8	Angle Rotor	5000	12x15ml	3080
4N-9	Angle Rotor	5000	4x50ml	2520
4N-10	Angle Rotor	5000	6x50ml	2850
4N-11	Angle Rotor	5000	4x100ml	2630
4N-12	Angle Rotor	5000	6x100ml	3130

  

TD4N is a table top centrifuge widely used to qualitative analysis of blood serum, plasma and urea in the fields of hospital, blood center, laboratory and biochemistry.