Propargyl-PEG8-bromide is a PEG reagent containing an alkyne group and a bromide group. The alkyne group can react with azide-bearing compounds or biomolecules in Click Chemistry reactions under the catalyzation of copper. The PEG spacer increases water-solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Propargyl-PEG8-bromide is a PEG reagent containing an alkyne group and a bromide group. The alkyne group can react with azide-bearing compounds or biomolecules in Click Chemistry reactions under the catalyzation of copper. The PEG spacer increases water-solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
Human Papillomavirus (HPV) (High and Low Risk) TaqMan PCR Detection Kits
Product Info
Document
Product Info
Overview
Detection kits for the HPV (High and Low Risk)
Available in TaqMan format for analysis
More than 70 types of human papillomavirus (HPV) have been identified, and are generally classified as high-risk or low-risk depending on their relationship or lack of relationship with cancer and high-grade cervical intraepithelial neoplasia (CIN 2-3). HPV viruses are predominantly sexually transmitted and high-risk HPV types are a major risk factor for development of cervical cancer. Low-risk HPV types 6 and 11 have been associated with the presence of genital warts. There are many other low-risk HPV types that are not associated with genital warts or cervical cancer. Until now, HPV cannot be cultured in vitro, and immunological tests are inadequate to determine the presence of HPV cervical infection. On the other hand, biopsies can be analyzed by nucleic acid hybridization to directly detect the presence of HPV DNA. HPV 16 and HPV 18 have been considered as high-risk cancer associated HPV types. HPV types 31, 33, and 35 have been shown to have an intermediate relationship with cancer. Norgen’s Human Papillomavirus (HPV) (High and Low Risk) TaqMan PCR Detection Kits are suitable for the detection of HPV 33 and HPV 58.
HPV (High and Low Risk) TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
HPV (High and Low Risk) TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
For the isolation and differentiation of sorbitol-negative Escherichia coli serotype O157 from food and animal feed and other materials
Principle and Interpretation
Enzymatic digest of casein and enzymatic digest of animal tissue provides nitrogen source, vitamins and growth factors; sodium chloride maintains balanced osmoticpressure; sorbitol is a fermentable sugar; No. 3 bile salt and crystal violet inhibit Gram-positive bacteria, potassiumtellurite inhibits non-o157 Escherichia coli, and cefixime inhibits Proteus; neutral red is a pH indicator; and agar is the coagulant of the culture medium.
Formulation
Ingredients
/liter
Enzymatic digest of casein
17.0g
Enzymatic digest of animal tissue
3.0g
Sorbitol
10.0g
Bile salt No.3
1.5g
Sodium chloride
5.0g
Neutral red
0.03g
Crystal violet
0.001g
Agar
15.0g
pH 7.1±0.2 at 25°C
Preparation
Weigh 51.5g of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, dispense into Erlenmeyer flasks, and sterilize at 121℃ for 15min. Melt the sterilized culture medium and cool it to about 45℃, add 1 tube of supporting reagent (SR0240) (0.25mg potassium tellurite and 0.005mg Cefixime) to every 100mL of basal culture medium, mix and pour into the plate.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 18-24hours
Quality control strains
Growth
Colony color
Escherichia coli ATCC25922
PR≥0.7
pale pink to red
Escherichia coli 0157:H7 NCTC12900
PR≥0.7
colorless
Staphylococcus aureus ATCC 25923
Total inhibition
–
Sorage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Document
Intended Use For the isolation and differentiation of sorbitol-negative Escherichia coli serotype O157 from food and animal feed and other materials Principle and Interpretation Enzymatic ……
Everything you need to run a trial PACE Genotyping Reaction on your existing lab equipment. Each PACE Trial Kit includes Test DNA samples, PACE Genotyping Assays, PACE Master Mix and a comprehensive PACE Genotyping Trial Kit Manual.
About
Everything you need to run a trial PACE® allele-specific PCR Genotyping Reaction on your existing lab equipment. Each PACE Trial Kit includes Test DNA samples, PACE Genotyping Assays, PACE Master Mix and a comprehensive PACE Genotyping Trial Kit Manual.
WHO IS THIS TRIAL KIT FOR?
Anyone who wants to try PACE genotyping reagents in their lab for the first time with a set of validated DNA samples, SNP assays and PACE Master Mix.
TRIAL KIT OVERVIEW
Step 1. Dispense each of the three trial DNA samples (DNA 1, 2 and 3) plus water (No Template Control) in triplicate onto a PCR plate using the suggested volumes.
Step 2. Combine appropriate volumes of PACE Genotyping Master Mix with PACE Genotyping Assay in a tube, as directed, then mix.
Step 3. Dispense the combined mixtures into each of the wells containing DNA using volumes indicated. Each test now contains a complete PACE Genotyping Reaction.
Step 4. Seal your PCR plate with an optically clear seal and centrifuge to ensure all components are at the bottom of the wells.
Step 5.Thermally cycle the reaction plate using the thermal cycling conditions provided.
Step 6. Read the plate and compare data produced with the expected results provided in the manual. Simple!
PACE MECHANISM
More information on the PACE genotyping chemistry and how it works can be found here: www.3crbio.com/#pace. PACE allele-specific PCR is used for the detection of SNPs, Indels and other sequence variants.
REQUIRED COMPONENTS
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
PCR-grade water
Document
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal