Propargyl-PEG8-NHS ester is an amine reactive compound with an alkyne group. The alkyne group reacts with azide-containing biomolecules in Click Chemistry reactions, copper is needed as a catalyst. The hydrophilic PEG units helps the compound to have better solubility in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Propargyl-PEG8-NHS ester is an amine reactive compound with an alkyne group. The alkyne group reacts with azide-containing biomolecules in Click Chemistry reactions, copper is needed as a catalyst. The hydrophilic PEG units helps the compound to have better solubility in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
96-Well 0.2 mL PCR Strip Rack SBS/ SLAS Footprint
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Product Info
96-Well 0.2 mL PCR Strip Rack SBS/ SLAS Footprint
SKU: MTP96
The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750RMSPU650R or MSPU650 magnet plates and is available as a discounted kit
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The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750R MSPU650R or MSPU650 magnet plates and is available as a discounted kit
Extract total RNA (including microRNA) from FFPE samples
No phenol extraction step
Includes DNase for optional on-column DNA removal
Isolated RNA is of the highest quality and integrity
Isolate a diversity of RNA species
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s FFPE RNA Purification Kits provide a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kits provide conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. These kits are able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform.
FFPE RNA Purification Kit (Spin Column)
Maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Maximum loading volume of 400 μL per well, and a maximum binding capacity of 50 μg per well.
5 slices of < 20 µm thick paraffin slices 25 mg of unsectioned block
Average Yield
Variable due to age of paraffin blocks ~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney
* Time required for purification varies by length of Proteinase K incubation and formalin crosslink-reversal
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year from the date of shipment.
[DM2360] FluoroBand™ 100 bp+3K Fluorescent DNA Ladder, 500 μl
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Description
The DM2360 FluoroBand™ 100 bp+3K Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM2360 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Features
Sharp bands
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Directly observed by UV or blue light— premixed with high sensitive DNA fluorescent dye
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 3,000 bp
Concentration
56 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Protected from light Room temperature for 6 months 4°C for 12 months -20°C for 24 months
Document
The DM2360 FluoroBand™ 100 bp+3K Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM2360 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.