Functionally tested Protein A conjugated gold
Perfect for Lateral Flow Development and use in final product
40nm gold particles conjugated to Protein A are offered in 10 OD 1mL size. If a different OD or amount is required please
Product image shows functional testing of Protein A 40nm colloidal gold on a lateral flow test. Protein A 40nm colloidal gold was incubated in lateral flow running buffer alone or in the presence of a limiting amount of monoclonal antibody that targets the chemical-BSA conjugate. The gold was then applied to the sample port of a cassette containing a lateral flow strip sprayed with a chemical conjugate (Chemical-BSA) on the test line and a goat anti mouse antibody as the control line. The data demonstrates the specificity and robustness of the Protein A 40nm colloidal gold.
Our products are produced in a state-of-the-art manufacturing facility that enable rapid turnaround times while ensuring batch to batch consistency and reliability.
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Endotoxin Removal Kits- For DNA
Product Info
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Product Info
Overview
Reduce endotoxin levels to 0.1 EU/µg DNA or less
Available in 3 formats: Mini, Midi, and Maxi to suit your desired input volume
Remove endotoxins from up to 1 mg of DNA with the Maxi format kit
Fast and easy processing using a rapid spin-column format
These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen’s spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample.
Endotoxin Removal Kit (Mini)
This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes.
Endotoxin Removal Kit (Midi)
This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
Endotoxin Removal Kit (Maxi)
This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
About Endotoxins
Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Extract high quality & quantity total RNA including miRNA
No phenol step required – isolate all RNA in one fraction
Rapid processing in under 40 minutes
Isolate total RNA from a wide variety of specimens
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants, and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Purification Technology
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Buffer RL (please see the flow chart on page 4). Ethanol is then added to the lysate, and the solution is loaded onto a maxi spin column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Solution A. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.
Average Yield: HeLa Cells (5 x 107 cells) E. coli (2.5 x 1010 cells)
~750 μg ~1.5 mg
*For isolating total RNA from purified leukocytes
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
1. Microprocessor control, less noisily, it is widely used to qualitative analysis of blood serum, plasma and urea in the fields of hospital, laboratory and biochemistry.
2. Brushless motor, free maintenance, no powder pollution, quick in speed up and down.
3. Special program, HLA and SERO separately.
5. Micro computer control system, LED display the RCF，time and speed.
6.. Electric lid lock, compact design, super speed and imbalance protection.
TD4 Technical Parameter：
Max. Speed
4000rpm
Max. RCF
2000
Max. Capacity
12×7
Time Range
0~99min
RPM/RCF Convert
Yes
Noise (dB)
≤ 55
Temperature
Normal
Acc/Dcc
10 Kinds
Speed Accuracy
±20r/min
Temperature Accuracy
/
Voltage(V/Hz)
AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)
485×320×255mm
Net Weight(Kg)
24KG
Matched Rotors for TD4
Order No.
Rotor
Max Speed (rpm)
Max Volume(ml)
Max. RCF（×g）
NO31501
SERO Rotor
3000
12×7/5ml(10-13*60-100mm)
2000
NO31502
HLA Rotor
4000
12×2/1.5/0.5ml
1000
Rotor Name
Max RCF(×g)
Use
HLA rotor for Lymphocyte washing
2000
Lymphocyte separation incubated cell separation
1000
Hematoblast extenterate(thrombin disposal)
1000
Lymphocyte washing
SERO rotor for erythrocyte washing
500
Blood type determination, the image of the hematocyte
1000
The test of intersectant aptness
1000
Hematocyte washing, the distillation of the serum and plasma
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TD4 cell washer centrifuge it is widely used to qualitative analysis of blood serum, plasma and urea in the fields of hospital, laboratory and biochemistry.
Matched Rotors for TD4