Functionally tested Protein A conjugated gold Perfect for Lateral Flow Development and use in final product 40nm gold particles conjugated to Protein A are offered in 10 OD 1mL size. If a different OD or amount is required please
Product image shows functional testing of Protein A 40nm colloidal gold on a lateral flow test. Protein A 40nm colloidal gold was incubated in lateral flow running buffer alone or in the presence of a limiting amount of monoclonal antibody that targets the chemical-BSA conjugate. The gold was then applied to the sample port of a cassette containing a lateral flow strip sprayed with a chemical conjugate (Chemical-BSA) on the test line and a goat anti mouse antibody as the control line. The data demonstrates the specificity and robustness of the Protein A 40nm colloidal gold.
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Urine Exfoliated Cell and Bacteria RNA Purification Kit
Product Info
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Product Info
Overview
Purify all sizes of RNA (including microRNA) without the need for phenol
Isolate and detect total RNA from 1 mL and up to 50 mL urine
Provides high-quality RNA for sensitive applications – isolate RNA from as little as 100 cells
Rapid processing time from sampling to downstream testing
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
This kit provides a rapid spin column method for the isolation and purification of total RNA (including microRNA) from exfoliated cells that have been shed into the urine from the urinary tract. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, as well as from the contaminating species found in urine such as glucose and salts, without the use of phenol or chloroform. The purified RNA is of the highest integrity and can be used in a number of downstream applications including real-time RT-PCR, qRT-PCR, Northern blotting, NGS, and expression array assays. Multiple samples can be processed in 20 minutes.
Background
RNA biomarkers from exfoliated cells can be used as non-invasive tools for a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers.
~1μg RNA per 1 x 105 cells (For Exfoliated Cells – Varies due to cell density sample)~0.5μg RNA per 1 x 107 cells(For Bacteria – Varies due to cell density sample)
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
The 16S V3-V4 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V3-V4 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V3-V4 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V3-V4 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Details
Minimum amount of starting material:
2.5 µL of DNA (5 ng/µL)
Time to complete library preparation:
4 hours
Storage Conditions and Product Stability Norgen’s 16S V3-V4 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
For the cultivation of E. coli in fermentation and molecular genetic studies.
Principle and Interpretation
Peptone provides nitrogen . Vitamins (including B vitamins) and certain trace elements are provided by yeast extract. Glucose provides carbon.Sodium ions for transport and osmotic balance are provided by sodium chloride. Agar is the solidifying agent.
Formulation
Ingredients
/liter
Peptone
10.0g
Yeast extract
5.0g
Sodium chloride
5.0g
Glucose
1.0g
Agar
15.0g
pH7.0±0.2 at 25°C
Preparation
Suspend 36g in 1 litre of distilled water. Bring to a boil to dissolve completely. Sterilize by autoclaving at 121°C for 15minutes.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 24 hours
Quality control strains
Growth
Escherichia coli ATCC25922
good
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
