Introduction

This kit is suitable for high-throughput extraction of total RNA from paraffin embedded tissue samples, including miRNA. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 120 minutes. The obtained RNA can be directly used in RT-PCR. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 holeo scillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from FFPE tissue
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Products	RNA, miRNA
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Adaptive instrument	Nucleic acid extractor, pipetting workstation
Sample type	FFPE slice, FFPE embedded tissue
Sample amount	≤6 slices

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• Fast – several samples can be extracted in 120 minutes by column method
• Safe – no phenol chloroform extraction required

Kit Contents

Contents	IVD3022
Purification Times	200 Preps
MagBind Particles	4.5 ml
Proteinase K	100 mg
Protease Dissolve Buffer	6 ml
DNase I	4 x 600 µl
DNase Buffer	30 ml
Buffer DPS	200 ml
Buffer FRL	40 ml
Buffer AL	40 ml
Buffer MW1*	110 ml
Buffer MW2*	2 x 50 ml
Nuclease Free Water	30 ml

Storage and Stability

Proteinase K and MagBind Particles should be stored at 2–8°C upon arrival. DNase I should bestored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K and MagBind Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.

This kit is suitable for high-throughput extraction of total RNA from paraffin embedded tissue samples, including miRNA. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 120 minutes. The obtained RNA can be directly used in RT-PCR. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 holeo scillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.

DD6 Floor Standing 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

Application 

for DD6 Floor standing 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

Widely used in the area of laboratory, pharmaceutical factory, biochemistry, biological products and so on. It is the ideal instruments for blood separation, protein precipitation and cell collection.

Features 

for DD6 Floor standing 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

1. Brushless DC motor in great torque, free maintenance, no carbon dust pollution, quick in speed up and down.
2. Flexible axle driven system which drive the rotor directly, smooth in operation, low noise and small vibration.
3. Automatically electric lid lock, super speed, over temperature protection and imbalance protection.
4. Microprocessor control, speed, time, temperature and RCF in operation, speed rising and reducing quick, operate simply.
5.10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
6. There are many rotors for your choice, adapters are available by experiment requirements.
7.3 tiers protection steel cover, safe and reliable.

Technical Parameters 

for 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

Max Speed: 6000r/min	Max.RCF: 6600xg
Max Volume: 4x1000ml	Power supply:AC220V,50HZ,10A
Timer: 1~99min	Noise:≤55dBA
Dimension:800×645×830mm	Net Weight: 170KG
Speed Accuracy: ±20r/min	Package(W*D*H): 920×800×1320mm
Temperature range:	Inapplicable

Matched Rotors for DD6 laboratory centrifuge

Order No.	Rotor type	Max.Speed (rpm)	Max.Volume (ml)	Max.RCF (xg)
No33097	Swing out rotor	4,000	4x1000ml (round/square cup )	4,060
No30221	Angle rotor	6,000	4x300ml	5,390
No30222	Angle rotor	6,000	6x300ml	5,660
No30223	Angle rotor	6,000	6x500ml	6,600

Packing & Shipping_____________________

by Sea/ by Air / by Express Sevices DHL, Fedex, TNT…

DD6 is floor standing large capacity 4x1000ml, 6x300ml, 6x500ml laboratory centrifuge machine without refrigerated one. It is up to max.speed 6000rpm.

Overview

• No need to immediately process samples
• Nucleic acid preservation at room temperature over 2 years for DNA and up to 7 days for RNA
• Ship fecal samples at room temperature safely
• Compatible with most DNA/RNA isolation methods
• Optimized isolation procedure using Norgen’s Microbiome DNA Isolation Kit

Norgen’s Fecal Swab Collection and Preservation System is designed for collection, ambient storage and transport of nucleic acids from stool specimens. The Fecal Swab Collection and Preservation System tubes contain Norgen’s Stool Preservative in a liquid format. The user simply collects the specimen using the provided swab, and then transfers the swab into the Stool Preservative. The Stool Preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Stool Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Stool Preservative allow samples to be stored at room temperature for over 2 years for DNA and 7 days for RNA. To extend the stool RNA stability in the preservative, storage at -20°C or -70°C is recommended upon arrival at the testing facilities until RNA purification.

Free Download

Extracting Biological Insights from Stool

Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV’s from fecal samplesDownload for Free

Details

Supporting Data

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Kit Specifications
Stability of Stool Nucleic Acids at Room Temperature	2 years for DNA Up to 7 days for RNA*

* The RNA stability will vary depending on the samples

Storage Conditions and Product Stability

The Fecal Swab Collection and Preservation System (Bulk Format) can be stored at room temperature for up to 2 years without any reduction in kit performance. The expiry date for the swabs is written on the swab wrapper.

Kit Components	Cat. 45670-B
Individually Wrapped Swabs	50
Fecal Swab Collection and Preservation Tube	50
Product Insert	1