Introduction
The Kit provides a high throughput of high-quality RNA from 96 samples of cells, tissues, and yeast using silica-membrane spin column plate with a binding capacity of 100ug RNA. RNA purified using the HiPure Total RNA System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Details
Principle
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
Kit Contents
| Contents | R401601 | R401602 |
| Purification Times | 1 x 96 | 4 x 96 |
| HiPure RNA Plate | 1 | 4 |
| 1.5ml Collection Plate | 1 | 4 |
| 0.5ml Collection Plate | 1 | 4 |
| RTL Lysis Buffer | 100 ml | 400 ml |
| RNA Binding Buffer* | 30 ml | 120 ml |
| Buffer RW1 | 100 ml | 400 ml |
| Buffer RW2* | 50 ml | 2 x 100 ml |
| RNase Free Water | 20 ml | 80 ml |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
