R4016 HiPure Total RNA 96 Kit

The Kit provides a high throughput of high-quality RNA from 96 samples of cells, tissues, and yeast using silica-membrane spin column plate with a binding capacity of 100ug RNA. RNA purified using the HiPure Total RNA System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Introduction

Details

Principle

HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.

Kit Contents

Contents	R401601	R401602
Purification Times	1 x 96	4 x 96
HiPure RNA Plate	1	4
1.5ml Collection Plate	1	4
0.5ml Collection Plate	1	4
RTL Lysis Buffer	100 ml	400 ml
RNA Binding Buffer*	30 ml	120 ml
Buffer RW1	100 ml	400 ml
Buffer RW2*	50 ml	2 x 100 ml
RNase Free Water	20 ml	80 ml