Introduction
This product supplies a simple and rapid extraction of total RNA from tissue and culture cells samples. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction processis only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure LQ Kit buffers can be used for both manual extraction process and automatic nucleic acid extraction machines.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from tissue and cells and plant |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Sample type | Animal tissue, human tissue, cultured cells, lymphocytes, ordinary plant tissue |
| Sample amount | Cells: 1 x 107Animal tissue: ≤20mgPlant tissue: ≤100mgYeast cell: ≤5 x 106 |
| Yield | 5-100μg |
Principle
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Advantages
- High purity – OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
- Economy – less than 50% of the price of Qiagen and other imported products
- Automatic – extraction without manual participation, saving time and effort
- Universal – suitable for various animal tissues, cultured cells and conventional plant fungal tissues
Kit Contents
| Contents | R662201 | R662202 | R662203 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagPure RNA Particles | 1.7 ml | 4.0 ml | 18 ml |
| Proteinase K | 24 mg | 50 mg | 240 mg |
| Protease Dissolve Buffer | 1.8 ml | 5 ml | 15 ml |
| DNase I | 600 μl | 2 x 600 μl | 10 x 600 μl |
| DNase Buffer | 30 ml | 40 ml | 200 ml |
| RTL Lysis Buffer | 40 ml | 80 ml | 400 ml |
| Buffer MCB* | 18 ml | 30 ml | 150 ml |
| Buffer MW1* | 44 ml | 66 ml | 2 x 220 ml |
| Buffer RW2* | 20 ml | 50 ml | 2 x 100 ml |
| RNase Free Water | 10 ml | 30 ml | 120 ml |
Storage and Stability
MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I shouldbe stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles and Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for atleast 18 months under these conditions.
