This product is specially designed for stool RNA extraction. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.
Detail
Introduction
This product is specially designed for stool RNA extraction. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.
Details
Principle
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Kit Contents
Contents
R662601
R662602
R662603
Purification Times
48 Preps
96 Preps
5 x 96 Preps
MagPure RNA Particles
1.7 ml
4.0 ml
18 ml
DNase I
600 μl
2 x 600 μl
10 x 600 μl
DNase Buffer
30 ml
40 ml
200 ml
Buffer SPL
30 ml
60 ml
270 ml
Buffer PHC
30 ml
60 ml
270 ml
Buffer MCB*
9 ml
15 ml
75 ml
Buffer ALB3*
10 ml
20 ml
100 ml
Buffer GW1*
44 ml
66 ml
2 x 220 ml
Buffer RW2*
20 ml
50 ml
2 x 100 ml
RNase Free Water
10 ml
30 ml
120 ml
2ml Bead Tubes
48
96
5 x 96
Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Rapid AAV DNA extraction and quantification can be completed in 2 hours
Universal qPCR assay targeting the AAV2 ITR enables quantification of most AAV vector constructs
Purified DNA standard enables easy comparison of data between different lab groups
Non-plasmid based standard prevents quantification artifacts due to insufficient melting of plasmid sequences
DNAse digestion step aids in eliminating non-viral DNA
Minimal sample handling for maximum AAV DNA recovery
Norgen’s AAV Quantification Kit is designed for the detection of adeno-associated virus (AAV) inverted terminal repeat (ITR) sequences in a real-time PCR based on the use of TaqMan® technology. A purified standard based solely on the AAV2 ITR sequence purified using Norgen’s proprietary silicon carbide technology simplifies the generation of a reliable standard curve for AAV quantification. Avoidance of a plasmid based standard eliminates problems associated with efficient melting of the ITR sequence due to coupling of the ITR to the much longer plasmid sequence, as well as variability due to rearrangements/duplications/deletions of the recombination prone ITR. An easy and rapid method for viral DNA extraction simplifies the step of obtaining AAV DNA while simultaneously eliminating contaminating non-encapsidated DNA. Norgen’s AAV Quantification Kit can facilitate pre-clinical studies that require accurate vector titration as well as interlab comparisons of vector quantities. This kit is designed for research use only and not for use in diagnostic procedures.
Storage Conditions and Product Stability All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.
Compact and stylish designed, the BioShake XP high-speed microplate mixer lets you perform all your standard mixing runs with a minimum of adjustments. The XP offers outstanding performance to handle a wide range of applications across biotechnology, pharmaceutical and academic research.
Small. Light. Efficient. Quiet.
Ideal for microplates & PCR plates
High-precision mixing from 200 up to 3,000 rpm
Universal spring clamps for easy fixation
Wide range of adapters
Stylish, fire resistant aluminium housing
ADVANCED TECHNOLOGY
Compact & stylish designed
Mixing tasks in microplates can be realized fast & safe with using adjustable speed of 200 up to 3,000 rpm.
The BioShake mixing-technology is obviously more robust, vibration free and needs less maintenance compared to classical mixers.
The first-class finished aluminium housing gives the BioShake XP its essential functionality. It provides a high amount of security, device stability and ensures a long service life.
GENTLE MIXING OF SAMPLES
By planar orbital motion
German designed and manufactured, BioShake XP plate mixer offer an ultra-efficient, 2-dimensional shaking axis so that samples mix completely in a fraction of the time of competing systems.
The mixing orbit of 2.0 mm is always constant. The orbital shaking is precisely controlled, in fact, that you need never spin down your plates after mixing. Even tubes, vials, high density plates, or low sample volumes, offer no obstacle for these precision tools.
Fully adjustable between 200 rpm and 3,000 rpm, well beyond the speeds of most other brands, guarantees fast, splatter-free, mixing for tubes, glass vials or across an entire 384-well microplate.
COMFORT
Simple intuitive operation and programming
The BioShake XP is operated from the front face of the enclosure which includes touch buttons and a display.
Special buttons for start, stop and storage of time and mixing modes enable the instrument to run complex applications. This opens new points of view on the daily laboratory work and optimizes routine application enormously.
The short mix button allows short and fast mixing in between.
The two line LCD display guarantees simultaneous and safe reading of all programmed and measured parameters as time and mixing frequency.
UNIVERSAL SPRING CLAMPS
Suitable for all microplates
In the corners eight spring clamps are inserted for easy fixation of skirted microplates anddeep well plates in SBS format 127.7 x 85.5 mm without an attached adapter. These spring clamps lock the microplate quickly and safely, even at the highest mixing frequencies.
For flexible and deformable PCR plates we recommend using of the related PCR adapter.
Fast mixing processes with frequent plate changes can be performed in the routine as easy and safe.
UNIQUE DESIGN MEETS HIGH-END TECHNOLOGY
First-class finished aluminium housing
Unique and efficient design combined with the most compact housing result in beautiful and well-defined laboratory equipment. Therefore, the first-class finished aluminium housing gives the BioShake XP its essential functionality. It provides a high amount of security, device stability and ensures a long service life.
Safety of the user and ease of use are clearly a priority for us.
EXCHANGABLE ADAPTERS
For a wide range of applications
The BioShake XP comes with a variety of standardized and specific adapter plates. The replacement of the adapters are very simple.
Perfect shaped adapters allow an optimal fit for standard tubes, lysis tubes, microplates, glass vials and other sample vessels.
MADE IN GERMANY
High-end instruments for daily routine
A perfectly harmonious blend of high-tech and handmade. “Made in Germany” has always been a recipe for success for QInstruments. 100% of QInstruments production takes place in Germany.
The focus is on human diligence and on being environmentally friendly. The company upholds its quality guarantee by means of consistent quality management. For more than 15 years these lab automation professionals from Jena have used only high-quality materials to ensure sustainable production, applied innovative thinking and undertaken research in a future-oriented way – all as a matter of course.
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Ideal for microplates & PCR plates
High-precision mixing from 200 up to 3,000 rpm
Universal spring clamps for easy fixation
Wide range of adapters
Stylish, fire resistant aluminium housing
