Ractopamine ELISA Test Kit is a competitive enzyme immunoassay for detection in meat, feed, liver, kidney, milk, urine, serum and plasma. Ractopamine has been banned for use as a livestock growth promoter in many countries around the world. To prevent ractopamine residues from entering the food chain, both producers and government surveillance agencies need technologies and methods that can provide rapid, accurate and reliable detection at specific sensitivities. This kit enables government agencies, food manufacturers, as well as quality assurance organizations, to detect ractopamine as low as 0.1 ng/g or ppb level in a variety of sample types.
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ProteoSpin™ On-Column Proteolytic Digestion Kit
Product Info
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Product Info
Overview
Rapid and simple procedure to generate digested peptides
Simultaneous digestion, purification and concentration at once
Peptide generation is complete, with no generation of additional artifacts being detected in mass spectrometry
Peptides are ready for applications such as mass spectrometry and SDS-PAGE
Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
This kit is highly efficient in the enzymatic digestion of simple and complex protein samples using trypsin and the subsequent purification of the resulting peptides using a convenient spin column format. Trypsin is added to the protein sample and bound to the column. Salts are washed away and the trypsin is then activated to digest proteins. Peptides are then eluted in a small volume and ready for downstream analysis. The peptides generated are complete, with no additional artifacts being detected in mass spectrometry. Fifteen micrograms of protein can be processed, digested and purified with each spin column with about 20 minutes of hands-on time (plus trypsin incubation). The simultaneous protein digestion and volumetric concentration of the purified peptides makes the kit a convenient method for preparing peptides to be analyzed by many downstream applications such as mass spectrometry and more.
Storage Conditions All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
Herpes Simplex Virus 1 (HSV-1) is a member of the herpes virus family, Herpesviridae. HSV-1 has a relatively large double-stranded DNA genome. HSVs are primarily transmitted by sexual intercourse, direct contact with lesions or perinatally. Most HSV positive cases are characterised by lesions on the skins and mucous membranes of the mouth and genitals. HSV infection can be either primary or a recurrence of a previous infection. More than 90% of the primary HSV infections are asymptomatic. Primary infection with HSV-1 can lead to gingivostomatitis, eczema herpeticum, keratoconjunctivitis and encephalitis. The primary symptoms of a secondary infection are skin lesions in the nose, mouth and genital regions. The infection is contagious, mainly during an epidemic.
HSV-1 TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
HSV-1 TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
The HiPure Plasmid DNA Mega Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 10 mg high copy number plasmid DNA can be purified from 500 mL overnight culture.
Details
Specifications
Features
Specifications
Main Functions
Isolation up to 10mg endotoxin-free plasmid DNA from 500ml bacterial culture
Applications
Cell transfection, animal injection, etc.
Purification method
Mega spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
High copy plasmid vector
Sample amount
500ml LB
Yield
1~10mg
Elution volume
≥2ml
Time per run
≤70 minutes
Liquid carrying volume per column
50ml
Binding yield of column
10mg
Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast – silica gel column purification is much faster than ion exchange
High yield – up to 10mg plasmid can be binded in one column
Low endotoxin – the obtainedplasmid can be directly used for cell transfection and animal injection, etc.
Kit Contents
Contents
P111602
P111603
Purification Times
10 Preps
50 Preps
RNase A
60 mg
2 x 150 mg
Buffer E1
220 ml
2 x 550 ml
Buffer E2
220 ml
2 x 550 ml
Buffer E3
220 ml
2 x 550 ml
Buffer E4
220 ml
2 x 550 ml
Buffer E5
120 ml
550 ml
Buffer PW2
25 ml
2 x 100 ml
Elution Buffer
30 ml
120 ml
HiPure EF Maxi Columns
10
50
Lysate Clear Maxi Syringe
10
50
50 ml Collection Tubes
20
100
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
The HiPure Plasmid DNA Mega Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 10 mg high copy number plasmid DNA can be purified from 500 mL overnight culture.