Description
Features of the sDNASEQ E.coli Residual DNA
Quantitation kit include:
Simpler and Rapid
- Only three steps will be need for Sample Preparation and All components of the Sample Preparation Kit can be stored at room temperature.
- Only one Reagent for qPCR;
- Only 1.5 hours will be needed for the whole test.
Accurate
- Perfect amplification curve, good amplification efficiency and good precision.
- Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology.
- Limit of Detection (LOD): 1 pg/mL; Limit of Quantification (LOQ): 5 pg/mL
- The recovery rate of different concentration samples in the linear range is between 70% and 130%
Kit Performance
Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.
Fig 2. Seven concentration samples of 5fg/μL, 10fg/μL, 20fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 30%, Regression coefficient associated with standard solutions was 0.99975, and amplification efficiency was 100.068%.
Fig 3. Four concentration samples of 5fg/μL, 10fg/μL, 20fg/μL, and 30fg/μL were detected, and 10 multiple holes were detected for each concentration. The detection values of 5fg/μL and above were CV ＜30%.
Fig 4. DNA recovery can be determined by including samples spiked with known DNA amounts which are prepared from the corresponding DNA standards. Typically, the range for this value varies from 70% to 130%.
Fig 5. Only one Reagent for qPCR MIX.
