Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
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SD-25/SD-25C Vacuum Centrifugal Concentrator
Product Info
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Product Info
SD-25/SD-25C Vacuum Centrifugal Concentrator
Ambient temperature type/Refrigerated type
Technical Parameter
Temperature range
Room Temperature~+80℃
-10℃~+80℃
Temperature Accuracy
士1℃
Max.Speed
2500rpm
Max.Capacity
6x100ml
Timer
0~99h59min
Vacuum control range
0.1~1000mbar
Main unit withstands vacuum levels
0.01mbar
Noise
≤55dBA
≤65dBA
Voltage
0.7kw
1.3kw
Power supply
220v,50Hz
Dimension(L×W×Hmm)
360*448*304mm
440*653*420mm
Adapter Rotor
Rotor Type
Rotor Volume
Rotor Type
Rotor Volume
Angle Rotor
42×1.5/2ml
Angle Rotor
8×50ml
Angle Rotor
108×1.5/2ml
Angle Rotor
6×100ml
Angle Rotor
48×5ml
Angle Rotor
16*20ml Sample vial
Angle Rotor
30×10ml
Angle Rotor
16*5ml+8*4ml+8*3ml
Angle Rotor
24×15ml
Microplate Rotor
2×96well
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Key features： 1. High-definition LCD touch screen, simultaneously displaying operational parameters: speed, temperature, vacuum, and running time. 2. Delayed start: after reaching a fixed speed, Start the vacuum pump to effectively prevent sample mixing. 3. Fully automatic vacuum control system, vacuum setting range0.1~1000mbar, Control accuracy0.1mbar. 4. 1.5/2ml Rotor stacking for simultaneous use; rotor replacement without tools. 5. Programmable with temperature and pressure settings; capable of storing 36 programs 6. Compatible with various rotor heads; transparent glass window for easy observation. 7. Magnetic drive: maintenance-free, non-contact drive, fully sealed. 8. Safety in use: inductive electric self-suction door lock ensures the centrifugal concentrator can only start when the door cover is fully closed and locked. 9. Can be used in conjunction with freeze-drying systems Model: SD-25/SD-25C
The Bisulfite Sequencing Library Prep Kit (illumina platform) was developed for construction of NGS libraries using bisulfite treated DNA (50 ng – 500 ng) as input. DNA methylation is an epigenetic mechanism known to play a critical role in gene regulation and genomic imprinting by blocking transcription factor access to promoters and enhancers. Bisulfite sequencing is a popular technique in biomedical research based on C to T conversion under the treatment of sodium bisulfite.
Recently, NGS became a powerful tool to identify the DNA methylation status at the whole genome level with single-base resolution. However, it is well known that bisulfite treatment of the NGS libraries causes tremendous damage to the libraries.
Bisulfite-Seq kit comparison
In the case of the regular bisulfite seq library preparation (library prep before bisulfite conversion), the DNA shearing equipment and the expensive methylated adaptors are required. In addition, the subsequent bisulfite conversion causes tremendous DNA damage to the constructed libraries.
BioDynami has developed a unique library prep technology to solve the problems. The technology uses bisulfite treated DNA as input to avoid the significant library loss caused by bisulfite conversion. Furthermore, DNA shearing step and expensive methylated adaptors are not required with our kit. The DNA polymerase in the kit has high-fidelity amplification ability and uracil tolerance which is ideal for amplification of bisulfite sequencing libraries. The final library is strand specific.
Bisulfite Sequencing Library Prep Kit Workflow
Three index types are available for the kit:
Non-index (Cat.# 30091): Libraries do not have index.
Index (Cat.# 30092): Each primer contains a unique barcode sequence of 6 bases to identify the individual library. Library multiplexing capacity is up to 48 samples. Index information can be downloaded here.
Unique dual index (Cat.# 30093): The multiplexing of bisulfite sequencing library is up to 96 samples with unique dual indexes. We used a Four-Base Difference Index System to generate indexes that have at least 4 bases different from each other in the 8-base index. The index primers remove NGS errors including index cross-contamination, index hopping, reads mis-assignment etc. Index information can be downloaded here.
Kit advantages
Easy and Quick protocol
Easy: Hands-on time only 10 minutes
Quick: Total protocol time around 1.5 hours
Simple workflow: Less steps
Directional library
Save magnetic beads more than 50%
Guaranteed high Bisulfite sequencing library conversion efficiency
Bisulfite treated DNA as input: From 50 ng to 500 ng
Document
The Bisulfite Sequencing Library Prep Kit (illumina platform) was developed for construction of NGS libraries using bisulfite treated DNA (50 ng – 500 ng) as input. DNA methylation is an epigenetic mechanism known to play a critical role in gene regulation and genomic imprinting by blocking transcription factor access to promoters and enhancers. Bisulfite sequencing is a popular technique in biomedical research based on C to T conversion under the treatment of sodium bisulfite.
