Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
High Sensitivity RNA Amplification With Improved Detection Isothermal Kit
Product Info
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Product Info
Product Description
High Sensitivity RNA Amplification With Improved Detection Isothermal Kit
Product Detail
Applicable equipment:
It is recommended to use the Isothermal fluorescence detector developed by Amp-future, which is also suitable for fluorescence quantitative PCR apparatus with market known brands.
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins,the recombinase and primers form a complex;Source search and combine the target homology domain, at this time,a D-loop region is formed at the homology position and strand exchange begins;along with the dissociation of the recombinase from the complex,the polymerase also binds to the 3′ end of the primer and begins chain extension. At the same time, relying on the function of exonuclease, adding specific molecular probes designed according to the template, and using fluorescence monitoring equipment can realize real-time monitoring of the amplification process of the target fragment.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
Technical Parameters:
Parameters
Details
Product Name
DNA Isothermal Amplification Kit EXO
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(fluorescent type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
Fluorescent Probe:Require the suitable length is 46-52nt.
DNA fluorescent kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
Document
It is recommended to use the Isothermal fluorescence detector developed by Amp-future, which is also suitable for fluorescence quantitative PCR apparatus with market known brands.
The Heat&Run® gDNA removal kit removes contaminating gDNA from RNA prior to RT-qPCR.
The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at low temperatures (5 minutes at 58ºC or 15 minutes at 55ºC).
Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.
Protocol
The kit contains HL-dsDNase which efficiently removes gDNA from RNA preps before running RT-qPCR.
gDNA is removed, leaving RNA ready for reverse transcription in the same tube (Figure 1).
Heat-labile dsDNase can easily be inactivated.
This procedure minimizes pipetting steps and reduces hands-on time.
The Heat&Run kit is especially well suited for high throughput experiments.
The high activity of the HL-dsDNase at low temperature and its heat-lability makes it ideal to use in a combination with a heat activated RT enzyme. The contaminating genomic DNA is cleaved at ambient temperature and increasing the temperature over 50°C will inactivate the DNase and start the Reverse Transcriptase.
Do you require gDNA removal in applications other than RT-qPCR? Contact our support team for assistance in implementing dsDNase treatment in your workflow.
Kit Contents
10X reaction Buffer
HL-dsDNase (50 or 250 reactions)
Document
The Heat&Run® gDNA removal kit removes contaminating gDNA from RNA prior to RT-qPCR.
The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at low temperatures (5 minutes at 58ºC or 15 minutes at 55ºC).
Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.
Propargyl-PEG6-NHS ester is an amine reactive linker. The alkyne in this linker can react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reaction. The PEG spacer increases solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG6-NHS ester is an amine reactive linker. The alkyne in this linker can react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reaction. The PEG spacer increases solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
