The RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor which possesses very high affinity for eukaryotic pancreatic-type ribonuclease. The RNAok™ RNase Inhibitor forms a 1:1 complex with pancreatic RNase A by noncovalent binding, presenting a noncompetitive inhibitory activity on these pancreatic enzymes. RNAok™ RNase Inhibitor is active against RNase A, RNase B, RNase C but not RNAse H, RNase I, RNase T1, RNase T2, and S1 nuclease. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV, M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase.
Detail
Description
The RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor which possesses very high affinity for eukaryotic pancreatic-type ribonuclease. The RNAok™ RNase Inhibitor forms a 1:1 complex with pancreatic RNase A by noncovalent binding, presenting a noncompetitive inhibitory activity on these pancreatic enzymes. RNAok™ RNase Inhibitor is active against RNase A, RNase B, RNase C but not RNAse H, RNase I, RNase T1, RNase T2, and S1 nuclease. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV, M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase.
Application
cDNA synthesis
in vitro translation
in vitro transcription
One-step RT-PCR
Separation and identification of specific ribonuclease activities
Storage Buffer
40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT, 0.1 mM EDTA, stabilizer and 50% (v/v) glycerol
Storage
-20°C for 24 months
Other Products
PlexTaq® 5x qPCR Multiplex Master Mix
Product Info
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Product Info
All in One. All the flexibility you need in a 5x Master Mix for multiplexing applications. NOW LYO READY!+ Sensitive. More free volume. 5x concentration allows more volume for target specific primers and probes (multiplexing). + Robust. Uniform amplification. Up to 30 target multiplexing in real-time (customer feedback). + Fast TTR. No extraction needed. Reliable results with crude samples without extraction step, like blood. + Specific. No false amplification. Engineered Taq DNA polymerase more stable at room temperature. Aptamer-based hot-start prevents false amplification and provides a fast-start function. + Lyo ready. Contains all necessary excipients needed for freeze-drying. Can be used for lyophilization. Enables RT storage and shipping once dried. Can be freeze-driedby us.Learn MoreFor research use and further manufacturing. Designed and manufactured under ISO13485.
Exemplary tetraplex PCR assay
Detection of three specific pathogen targets and an internal control. Total input DNA per reaction was 10^6 (red), 10^5 (yellow), 10^4 (blue), 10^3 (green), 10^2 (purple), 10 (light blue) and 0 copies (grey) and 8000 copies/reaction for the internal control (shown in the CY5 plot).
5x Multiplex – robust PCR perforemance for a wide range of qPCR application
PlexTaq 5x qPCR Multiplex Master Mix contains all components necessary for rapid, sensitive and reproducible quantification of DNA and cDNA. An engineered DNA polymerase and an optimized buffer including ultrapure dNTPs are key components of the ready to use mix. A hot-start formulation of the included DNA polymerase prevents aptamer prevents false amplification and provides a fast start function.
PlexTaq®´s formulation allows to use it also for direct PCRs from crude samples.
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All in One. All the flexibility you need in a 5x Master Mix for multiplexing applications. NOW LYO READY!
+ Sensitive. More free volume. 5x concentration allows more volume for target specific primers and probes (multiplexing).
+ Robust. Uniform amplification. Up to 30 target multiplexing in real-time (customer feedback).
+ Fast TTR. No extraction needed. Reliable results with crude samples without extraction step, like blood.
+ Specific. No false amplification. Engineered Taq DNA polymerase more stable at room temperature. Aptamer-based hot-start prevents false amplification and provides a fast-start function.
Human Cytomegalovirus (HCMV) or Human betaherpesvirus 5 (HHV-5) is a member of the Family Orthoherpesviridae. HCMV belongs to the betaherpesvirinae subfamily, while other herpesviruses fall into the subfamilies of Alphaherpesvirinae (including HSV 1 and 2 and varicella) or Gammaherpesvirinae (including Epstein-Barr virus). All herpes viruses share a characteristic ability to remain latent within the body over long periods of time. While CMV can be found in numerous body fluids including urine, saliva, breast milk, blood, tears, semen, and vaginal fluids, urine samples are generally used for congenital HCMV infection screening due to high viruria observed in infected infants.
CMV TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
CMV TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
D-Galactose, Raffinose
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
4 to 83 µg of D-galactose per assay (i.e. approx. 12 to 250 µg of raffinose per assay)
Limit of Detection:
21 mg/L
Reaction Time (min):
~ 60 min
Application examples:
Cereal flours, soybean flour, by-products of sucrose manufacture and other materials.
Method recognition:
Used and accepted in food analysis
The Raffinose/D-Galactose test kit allows for the specific and rapid measurement of raffinose and D-galactose in plant materials and food products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
