Introduction

This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.

Details

Specifications

Features	Specifications
Main Functions	IVD5412 precast kit for MagMix 32, smart 32
Applications	RT-PCR，PCR，NGS
Products	Viral DNA / RNA, body cell DNA / RNA
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technolog
Process method	Manual or automatic
Sample type
Sample amount	200μl
Adaptive instrument	Nucleic acid extractor, pipetting workstation

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.

Advantages

Kit Contents

Cat.No	Reagent	IVD5412-TL-96
PK/Carrier RNA		24mg/310μg
Protease Dissolve Buffer Blue		1.8 ml
Tip		12
Prepackage Plate(2.0ml Plate)	Row 1/7：500µl Buffer MLBRow 2/8：500µl Buffer MW1Row 3/9：500µl Buffer CWRow 4/10：500µl Buffer CW&MPNRow 5/11：100µl Buffer AVE	6

Storage and Stability

This kit is shipped and stored at room temperature and is valid for 12 months.

This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.

Name of Product

Lactobacillus/Pediococcus-Screen

Catalog Number

MGScLP

Short Info

This test discovers beer germs in a timely and precise manner

Method/Platform

PCR

Range/Assay Sensivity

10^4 – 10^5 cfu/mL

Test Principle

The technological basis for the GenLine tests  is the polymerase chain reaction (PCR) combined with lateral flow tests.

Labelled specific primers are used to amplify specific DNA fragments. In addition to the target gene, a control gene, which is also present in the PCR mixes, is amplified in order to make sure that the PCR process works properly.

The resulting PCR products carry the labels of the incorporated primers.

In a second part of the test, the created PCR products are detected by a lateral flow Test Strip.  A “molecular sandwich” is formed and becomes visible as a line on the test Strip.

Brief Instructions
The PCR reagents and the samples are prepared.
After the addition of the sample to the PCR reagents, the PCR is started.
The resulting PCR products are detected by a simple lateral flow test

Storage
-15 to -20°C

Components
PCR-Polymerase Master-Mix, PCR-Primermix, Positive Kit-Control, PCR-Water

Introduction

Usages:
For monitoring and detection of planktonic bacteria and settlement bacteria in pharmaceutical industry & clean room.

Advantage:

1.This series of products was filling at A level of environment, and final sterilization by Gamma ray irradiation, triple packaging insure sterility and long shelf life.
2.Each dish was marking label product name, batch number, expiration date .Information is available of traceability.
3.Inner additional desiccant, reducing formation of condensation water, while inner additional sterile paper and plastic bags for easier transfer and cultivation.
4.Triple packing dense bags to avoid the penetration of hydrogen peroxide; clean gas was filled as a buffer to reduce broken bags and dish in transit.
5.The 90mm medium plate loading capacity of 30mL, to meet requirement of the environmental sampling for long exposure of up to 4h and 5-7 days of culture.
6. This series of products is available to store at (2-25 ℃), shelf life of up to 6 months.

Storage: Store in a cool (2-25 ℃), dry place, away from bright light. Storage period of 6 months.

Specifications: 90mm*10 plates / bag

90mm*10 plates / bag