The kit is developed for RNA quantification. The RNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and two RNA Standards. Simply dilute the HS Dye with the HS Dilution Buffer, add RNA sample, then read the concentration using the Qubit Fluorometer. The assay is accurate for RNA concentrations from 250 pg/µL to 100 ng/µL (Figure 1). The RNA Quantification High Sensitivity Kit has several advantages over traditional RNA quantitation such as stability, sensitivity, and contaminant tolerance.
Detail
The kit is developed for RNA quantification. The RNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and two RNA Standards. Simply dilute the HS Dye with the HS Dilution Buffer, add RNA sample, then read the concentration using the Qubit Fluorometer. The assay is accurate for RNA concentrations from 250 pg/µL to 100 ng/µL (Figure 1). The RNA Quantification High Sensitivity Kit has several advantages over traditional RNA quantitation such as stability, sensitivity, and contaminant tolerance.
Features
Optimized condition for using with the Qubit Fluorometer
Uses the Qubit RNA High Sensitivity assay setting
Cuts costs by 60%
The performance of the BioDynami RNA Quantification HS kit is nearly identical to that of Thermo Fisher’s Qubit RNA HS kit (figure below).
Common contaminants such as detergents, solvents, salts, free nucleotides, or protein are well tolerated in the assay (Table 1).
Qubit is a registered trademark of Thermo Fisher Scientific.
Other Products
D3187 HiPure HP Plant DNA Kit
Product Info
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Product Info
Introduction
HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 150 mg plant and fungal tissue
This product is based on silica column purification. The sample is lysed with CATB Buffer. DNA is released into the lysate. Cell debris, precipitated proteins and polysaccharides are removed by chloroform extraction. After adjust the binding condition, transfer to an adsorption column. DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Broad spectrum – suitable for extracting DNA from various plant samples
Fast – several samples can be extracted in 30 minutes by silica technology
Good repeatability – silica gel column purification technology can obtain ideal results every time
Kit Contents
Contents
D318702
D318703
Purification Times
50 Preps
250 Preps
Buffer PAL
60 ml
200 ml
Buffer GWP
60 ml
200 ml
Buffer DW1
30 ml
150 ml
Buffer GW2*
20 ml
50 ml
Buffer AE
20 ml
60 ml
HiPure DNA Mini Columns II
50
250
2 ml Collection Tubes
50
250
Storage and Stability
This product can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.
Urine DNA Isolation Kit for Exfoliated Cells or Bacteria
Product Info
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Product Info
Overview
Isolate genomic DNA from either exfoliated cells or bacteria found in urine
Isolate and detect genomic DNA from as little as 1 mL of urine and up to 50 mL urine
High quality DNA for sensitive applications
Rapid processing time
Norgen’s Urine DNA Isolation Kit for Exfoliated Cells or Bacteria is designed for the rapid isolation of either: 1) human genomic DNA from exfoliated cells that have been shed into the urine from the urinary tract; or 2) bacterial genomic DNA from urine samples. The kit allows for the isolation of DNA from 1 to 50 mL of urine. The genomic DNA isolated from exfoliated cells can be used in a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers. Bacterial genomic DNA from both human urine samples and urine samples from animals can be isolated with this kit in order to study the levels and types of bacteria that are present. The kit allows for the isolation of genomic DNA from both Gram negative and Gram positive bacteria, including E. coli, Proteus spp., Klebsiella spp., Enterobacter spp., Serratia spp., Pseudomonas spp, Clostridial ssp. and Leptospirosis spp.Chlamydia trachomatis and Neisseria gonorrhoeae.
Typical yields of human genomic DNA from exfoliated cells will vary depending on the cell density of the urine sample, which is affected by a number of factors including health, diet and sex of the individual donating the urine. Typical yields of bacterial genomic DNA will vary depending on the urine sample and the bacterial species, if any, present in the urine. Healthy humans generally have < 10, 000 CFU of bacteria per mL of urine, and this kit is sufficiently sensitive to isolate and detect DNA from even this minimal amount of bacteria. The genomic DNA purified with this kit is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications such as PCR, qPCR and Southern Blot analysis.
10 minutes (Plus a 30 minute incubation – Bacteria) (Plus a 15 minute incubation – Exfoliated)
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
Propargyl-PEG9-amine has a propargyl group and an amine group. The amine group reacts with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) to form amide bonds. The propargyl group reacts with azides to form triazole linkage with the presence of copper as a catalyst. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG9-amine has a propargyl group and an amine group. The amine group reacts with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) to form amide bonds. The propargyl group reacts with azides to form triazole linkage with the presence of copper as a catalyst. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
