RNase H activity in a convenient and sensitive colormetric assay that delivers results in real time. Great for Quality Testing for RNase H activity in enzyme preparations and determination of RNase H unit activity.
Attogene has developed the first of its kind and patent pending technology that uses a novel RNA/DNA substrate tagged on the 5′ and 3′ end with a Biotin that is attached to our streptavidin colloidal gold reporter molecules and a test line tag. In the absence of RNases H, the gold tagged RNA/DNA molecule will flow up the lateral flow strip and bind to the test line using an anti tag antibody (INDICATING that NO RNase H is detected). When RNases H is present or added, however, the RNA strand of the RNA/DNA substrate is cleaved, and the Biotin tagged 5′ and 3′ tags are spatially separated in solution cauing the test line to disapear.
This test can be used to rapidly and efficiently detect ribonucleases H activity and is a perfect tool for monitoring unit activity or residual RNAse H activity.
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
Key Features
User-friendly gel cassette:
Numbered and framed wells for sample loading
Labeled warning sign and green tape as reminder
Enhanced gel performance:
Enhanced band sharpness
Better resolution of small proteins
Stable for shipping at ambient temperature
Easy compatibility:
Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
Compatible with most popular protein electrophoresis systems
Storage and stability
Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.
Do not freeze Q-PAGE™ Precast Gels. Remove tape and comb before electrophoresis.
Keep Q-PAGE™ Precast Gels flat during storage.
Document
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
Solid Phase Reversible Immobilization magnetic beads consist of paramagnetic particles coated with carboxyl groups that reversibly bind DNA. They are used for DNA purification because they are fast, simple and efficient. We have developed our own beads technology that are different from other SPRI beads technologies.
Our Magnetic Beads(DNA & RNA Purification) combines BioDynami’s proprietary chemistries with reversible DNA-binding properties of magnetic beads. The magnetic beads, which are unique from other SPRI beads, are developed for effective nucleic acid purification by removing unwanted components such as salts, dNTPs, enzymes, primers, adapters, and other impurities. The beads are RNase free, can be used for applications of DNA, and even work with more sensitive RNA without any additional cost.
Our magnetic beads are optimized to selectively bind DNA fragments of 100 bp and larger, and RNA fragments of 200 bases and larger, similar to other SPRI beads such as AMPure® XP* and SPRIselect*. Purified DNA and RNA are suitable for downstream applications requiring high quality DNA and RNA, as the purified fragments are free of contaminants and impurities. The beads can be used for NGS library purification, PCR fragment cleanup, molecular cloning, or even nucleic acid concentration.
The beads can also be used for size selection of DNA fragments ranging from 150 bp to 800 bp by changing the bead-to-sample volume ratio and performing single or double-size selection. The beads are an ideal choice for NGS library preparation. They can be easily integrated into the standard workflow of NGS library preparation since the volume ratio is similar for protocols using standard magnetic beads.
Features:
Effective recovery of DNA and RNA samples
DNA fragments greater than 100 base pairs
RNA fragments greater than 200 bases
Removal of unwanted components and impurities
Fragment size selection for specific applications
Consistent single or double-size selection
Flexibility: compatible with manual and automated processing
Cost effective alternative to other beads such as AMPure® XP* and SPRIselect* with equivalent performance
* AMPure® XP and SPRIselect are trade marks of Beckman Coulter.
Magnetic beads recovery rate. dsDNA and ssDNA of genomic DNA, 1 kb DNA, and 200 bp DNA fragments were used. Total RNA was also tested.
Comparison of elution volume vs yield. 40 ul , 30 ul, and 20 ul of elution volume were used. BioDynami magnetic beads have better recovery rates at low elution volume when compared to other SPRI beads such as AMPure® XP*.
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
Mycobacterium tuberculosis is a pathogenic bacterial species belonging to the genus Mycobacterium, and is the causative agent of tuberculosis. Tuberculosis (TB) is a multifaceted disease and challenging public health problem in both industrialized and developing countries. According to the WHO, 8.8 million active cases of TB are diagnosed each year and of these, almost 2 million die. Once thought to be under control or even close to extinction, TB infection levels are rising and the threat is compounded by new, virulent and drug-resistant strains. Although most cases occur in the developing world (22 countries accounting for 80% of all global cases), increasing population mobility combined with facility of transmission means that no country is immune from the resurgence of TB. TB control programs are currently facing a number of constraints. Worldwide, fewer than 25% of all tuberculosis cases are detected. Of utmost concern is the absence of a timely and accurate test for the diagnosis of mycobacterial disease. Early diagnosis is crucial for the prevention of further spread of the disease.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
