RNase Inhibitor (10,000 units) Concentration: 40 units/μL Volume: 0.25 mL Storage Conditions: Store at 2°C – 8°C Inhibits: RNases A, B, and C Supplied in: 1X PBS with 0.05% Sodium Azide.
Attogene’ s RNase Inhibitor is a protein-based ribonuclease inhibitor which noncovalently binds and inactivates a wide variety of RNases in a range of temperature (37–65°C) and pH (5.5–8.5) conditions. This product is distinct from placental RNase Inhibitor protein in that it inactivates RNases I and T1 in addition to RNases A, B and C. NA2021 is distinct from RNase Inhibitor protein in that it is less expensive, have more robust interactions with RNAses.
MADE AND FUNCTIONALLY TESTED AT ATTOGENE (MADE IN AUSTIN TEXAS – USA)
Isothermal Amplification Kit: Freeze-Dried Reagent, Stable & Easy to Operate
Product Detail
Kit Storage and Term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal Nucleic Acid Principle Summary
The kit is based on rapid nucleic acid amplification technology at room temperature and constant temperature, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension.
Isothermal Nucleic Acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters
Details
Product Name
DNA Isothermal Amplification Kit Basic
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal Nucleic Acid Applications
Suitable for DNA isothermal rapid amplification kit(Basic type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
Colloidal gold probe:Require a sequence of 46-52nt in length
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
EXTRAClean RNA Clean-Up and Concentration Micro-Elute Kit
Product Info
Document
Product Info
Overview
Ensure optimal results for sensitive applications like NGS
Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
Concentration of small amounts of RNA into 8 μL
Ideal for concentrating RNA purified from exosomes, plasma, serum, urine, and other bodily fluids and any RNA samples initially purified in large volumes
Efficient RNA cleanup from enzymatic reactions – labeling, DNase treatment and in vitro transcription
Cleanup of RNA isolated using different methods, including phenol/chloroform extractions
Fast and easy processing using rapid spin-column format
Suitable for all sizes of RNA, from large rRNA down to microRNA (miRNA)
No phenol or chloroform extractions
Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary separation matrix.
Norgen’s EXTRAClean RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. The minimum recommended elution volume is 8 μL, which enables the concentration of small amounts of all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other reaction components such as proteins, RNases and nucleotides, without the use of phenol or chloroform. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays and next generation sequencing.
Details
Kit Specifications (Spin Column)
Maximum Column Binding Capacity
10 μg
Size of RNA Purified
All sizes, including miRNA and small RNA (< 200 nt)
The Opentrons Flex Gripper GEN1 attaches to the gantry and is capable of moving labware across the deck and on or off modules such as the Magnetic Block or Thermocycler. It can also access the additional slots to the side of the main deck to replenish tips or labware. The gripper can be used with any pipette configuration.
Included with the gripper is a calibration pin that enables automatic calibration to ensure positional accuracy. Currently, the gripper is optimized for use with the following labware items:
– NEST 2 mL 96 Deep Well Plate, V bottom – Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt – NEST 96 Well Plate Flat – All Opentrons Flex tip racks
Document
The Opentrons Flex Gripper GEN1 attaches to the gantry and is capable of moving labware across the deck and on or off modules such as the Magnetic Block or Thermocycler. It can also access the additional slots to the side of the main deck to replenish tips or labware. The gripper can be used with any pipette configuration.
Included with the gripper is a calibration pin that enables automatic calibration to ensure positional accuracy. Currently, the gripper is optimized for use with the following labware items:
– NEST 2 mL 96 Deep Well Plate, V bottom – Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt – NEST 96 Well Plate Flat – All Opentrons Flex tip racks
