[RP1000] ExcelRT™ Reverse Transcriptase, 200 U/μl, 20000 U

Overview

• Detection kits for the HBV
• Available in TaqMan format for analysis

The Hepatitis B virus (HBV) is mainly transmitted via blood or blood products. In addition, sexual, oral and perinatal infections are also possible. Early symptoms of the infection include appetite loss, vomiting and abdominal symptoms, with approximately 10-20% of those patients developing fever as well as rheumatoid joint and muscle pain. Jaundice, which may be accompanied by itching, will then develop within 2-14 days. Fulminant hepatitis then occurs in about 1% of all infected patients, which in severe cases may be fatal. Of those individuals infected by HBV, 5-10% will develop chronic liver inflammation which may progress to cirrhosis of the liver or, in the worst case, primary liver cell carcinoma. The HBV virus is a hepadnavirus that has a circular genome composed of partially double-stranded DNA.

HBV TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

HBV TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

Figure 1 / 3

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM29250 (100 rxns)	Cat. TM29210 (100 rxns)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
HBV Primer & Probe Mix	280 μL	280 μL
HBV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Overview

• Simultaneous concentration, desalting and buffer exchange of total urinary proteins
• No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

These kits concentrate urine proteins while simultaneously removing salts, urea, and other urine contaminants. There is no molecular weight cut-off and therefore the columns capture total urinary proteins and peptides of all sizes making them ideal for biomarker discovery work, differential expression of proteins in various diseases, or other diagnostic research. The columns are convenient, rapid and easy to use and thus offer significant time savings over classic dialysis protocols. The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the sample conveniently for downstream proteomic applications including SDS-PAGE, 2D Gels, MALDI-TOF, LC/MS, LC/MS/MS, whole protein mass spectrometry, western blotting, protein microarrays, and more.

ProteoSpin™ Urine Protein Concentration Micro Kit

Each spin column is able to concentrate and desalt up to 200 μg of urine proteins. Twelve samples can be processed in 20 minutes.

ProteoSpin™ Urine Protein Concentration Midi Kit

The ProteoSpin™ Urine Protein Concentration Midi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 1 to 5 mL inputs of urine. Each mini spin column is able to concentrate and desalt up to 3 mg of urine proteins in 30 minutes.

ProteoSpin™ Urine Protein Concentration Maxi Kit

The ProteoSpin™ Urine Protein Concentration Maxi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 2 to 20 mL inputs of urine. Each maxi spin column is able to concentrate and desalt up to 4 mg of urine proteins in 45 minutes.

Details

Supporting Data

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Kit Specifications
Maximum Urine Input Volume	20 mL
Maximum Recovered Protein	4 mg
Minimum Elution Volume	2 mL
Time to Process 4 Samples	45 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

Component	Cat. 17400 (25 preps)	Cat. 52300 (10 preps)	Cat. 21600 (4 preps)
Wash Solution C	60 mL	60 mL	130 mL
Binding Buffer A	4 mL	4 mL	8 mL
Elution Buffer C	8 mL	30 mL	30 mL
Protein Neutralizer	4 mL	4 mL	4 mL
Micro Spin Columns	25	–	–
Midi Spin Columns (assembled with collection tubes)	–	10	–
Maxi Spin Columns (assembled with collection tubes)	–	–	4
Collection Tubes	25	–	–
Elution Tubes (1.7 mL)	25	–	–
Midi Elution Tubes	–	10	–
Elution Tubes (50 mL)	–	–	4
Product Insert	1	1

TG18 Tabletop 21000rpm High Speed Low Speed Non-refrigerated Large Capacity Multi-purpose lab centrifuge 800ml /500ml bottles & 5/7/10/15/50ml tubes. And holding Angle Rotors like 24×1.5/2ml, 6x50ml, 12x15ml…in high speed separation smooth.

TG18 Universal Refrigerated Centrifuge Application:

It’s widely used in the field of animal and plant molecular biology, which is the ideal instrument for seperating the chemical substances in the cell and purifing the microorganisms, viruses, bacteira and subcelluar etc.

TG18 High Speed/Low Speed Centrifuge Features:

1. Max. Speed 21000rpm, max. volume 4x800ml, can be used for 5ml/10ml/15ml/30ml/50ml/100ml centrifuge tubes.

2. Brushless DC motor in great torque, no powder pollution, free maintenance, quick in speed up and down.

3. Can store 10 programs, automatically calculate and synchronously display the RCF data. Digital display indicates the speed, time, temperature and RCF. The parameters can be changed in operation, no need to stop the centrifuge which is quite convenient.

4. The centrifuge body is made of high-quality steel, and with high-quality steel centrifuge chamber, safe and reliable.

5.  Automatically electric lid lock, over speed/over temperature protection and imbalance protection.

6. There are many kinds of rotors for your choice, both in high speed and low speed.

TG18 High Speed/Low Speed Centrifuge Technical Parameters:

Max. Speed	21000rpm
Max. RCF	30910xg
Max. Capacity	4x800ml
Timer	0-99min
Speed Accuracy	±20rpm
Noise(DB)	≤65DBA
Voltage(V/HZ)	AC220V/110V,50HZ 10A
Dimension(LxWxHmm)	685x500x385mm
Net Weight(Kg)	70KG
Certificates	CE, ISO
Warranty	1 Year

TG18 High Speed/Low Speed Centrifuge Matched Rotors:

Order No.	Rotor Type	Max Speed(r/min)	Volume(ml)	Max. RCF(xg)
G18-1	Swing Rotor	4000	4x800ml	3450
G18-2	Microplate Rotor	4000	4x4x96well	2940
G18-3	Microplate Rotor	4000	2x4x96well	3210
G18-4	Microplate Rotor	4000	2x3x48well	2300
G18-5	Swing Rotor	4000	4x30x5ml vacuum tube	2840
G18-6			4x30x7ml vacuum tube	3140
G18-7			4x18x10ml vacuum tube	3140
G18-8	Swing Rotor	4000	Fat bottle	3830
G18-9	Fixed Rotor	16000	4x8PCR	15760
G18-10	Fixed Rotor	15000	6x8PCR	21420
G18-11	Fixed Rotor	16000	8x8PCR	17480
G18-12	Fixed Rotor	15000	12x8PCR	22930
G18-13	Fixed Rotor	15000	40×0.5ml	22920
G18-14	Fixed Rotor	21000	12×1.5/2ml	30910
G18-15	Fixed Rotor	16000	24×1.5/2ml	23440
G18-16	Fixed Rotor	14000	30×1.5/2ml	20800
G18-17	Fixed Rotor	13000	48×1.5/2ml	17930
G18-18	Fixed Rotor	16000	16x5ml	22020
G18-19	Fixed Rotor	16000	6x10ml	21500
G18-20	Fixed Rotor	15000	12x10ml	22680
G18-21	Fixed Rotor	13000	16x10ml	19490
G18-22	Fixed Rotor	13000	8x15ml	17790
G18-23	Fixed Rotor	11000	12x15ml	14330
G18-24	Fixed Rotor	5000	24x15ml	3500
G18-25	Fixed Rotor	5000	30x15ml	3830
G18-26	Fixed Rotor	14000	6x30ml	19060
G18-27	Fixed Rotor	13000	6x50ml(conical)	18840
G18-28	Fixed Rotor	13000	6x50ml(round)	18730
G18-29	Fixed Rotor	5000	12x50ml	3860
G18-30	Fixed Rotor	4000	24x50ml	2970
G18-31	Fixed Rotor	13000	4x85ml	18940
G18-32	Fixed Rotor	12000	4x100ml	14850
G18-33	Fixed Rotor	10000	6x100ml	11380
G18-34	Fixed Rotor	4000	12x100ml	2970
G18-35	Fixed Rotor	12000	24 capillaries	15800
G18-36	Swing Rotor	15000	4x5ml	19920
G18-37	Vertical Rotor	16000	16x5ml	16540
G18-38	Vertical Rotor	14000	8x30ml	19750

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