[RP1000] ExcelRT™ Reverse Transcriptase, 200 U/μl, 20000 U
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The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase – an RNA dependent DNA polymerase capable of generating first strand cDNA using an RNA template. It is designed to reduce RNase H activity and create better thermal stability. The ExcelRT™ Reverse Transcriptase is able to routinely synthesize first strand cDNA >8 kb at 37~50°C.
Detail
Description
The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase – an RNA dependent DNA polymerase capable of generating first strand cDNA using an RNA template. It is designed to reduce RNase H activity and create better thermal stability. The ExcelRT™ Reverse Transcriptase is able to routinely synthesize first strand cDNA >8 kb at 37~50°C.
Features
High yield
Thermostable, up to 50°C, during first strand synthesis
High processivity, generating cDNA up to 8 kb
Reduced RNase H ribonuclease activity
Application
Generation of first strand cDNA from total RNA or mRNA.
Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.
Storage
-20°C for 24 months
High yield
Thermostable, up to 50°C, during first strand synthesis
20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol
5X RT buffer
250 mM Tris-HCl (pH 8.3 at 25°C), 375 mM KCl and 15 mM MgCl2
Storage
-20°C for 24 months
Other Products
Taenia solium – IgG ELISA
Product Info
Document
Product Info
12 x 8 strips (96 tests)
Cysticercosis is caused by larval cysts of the tapeworm Taenia solium and is a major cause of adult onset seizures in most developing countries. Diagnosis is based on imaging findings, but in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. The ELISA kit hs shown appropriate performances for the serological screening of cysticercosis.
Name of Product
Taenia solium – IgG ELISA
Catalog Number
AF 9700
Short Info
Cysticercosis is caused by larval cysts of the tapeworm Taenia solium and is a major cause of adult onset seizures in most developing countries. Diagnosis is based on imaging findings, but in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. The ELISA kit hs shown appropriate performances for the serological screening of cysticercosis.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
98% sensitivity, 98% specificity
Test Principle
The kit provides all the material needed to perform 96 enzyme-linked immunosorbent assays (ELISA) on breakable microtitration wells sensitized with Taenia solium cyst soluble antigens. Specific antibodies in the sample will bind to these antigens and washing will remove unspecific antibodies. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of pNPP substrate which turns yellow in the presence of alkaline phosphatase. Color intensity is proportional to the amount of Taenia solium specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader. The test can be performed with automatic systems, but this must be validated by the user.
Document
Cysticercosis is caused by larval cysts of the tapeworm Taenia solium and is a major cause of adult onset seizures in most developing countries. Diagnosis is based on imaging findings, but in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. The ELISA kit hs shown appropriate performances for the serological screening of cysticercosis.
IST-121 QuickSeal qPCR CrystalTM Self Adhesive Sealing Film
Product Info
Document
Product Info
Overview
Optically clear adhesive film with pressure activated adhesive. Seal is peelable; suitable for qPCR and other imaging techniques including crystallization.
Optically clear seal specifically developed for optical applications, particularly qPCR
It is non sticky when removed from the packaging; this aids handling when wearing gloves
The adhesive is contained within small capsules, allowing light to pass through to ensure the optical clarity of the seal
When the seal is in position, pressure can be applied to burst the capsules, releasing a strong adhesive only where the seal touches the raised well rims of the plate – the rest of the seal area above the wells remains optically clear
For all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer
Document
Optically clear adhesive film with pressure activated adhesive. Seal is peelable; suitable for qPCR and other imaging techniques including crystallization.
Opentrons OT-2 Tips, 20µL. Optimized for volumes 1µL to 20µL. Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.
Compatible with: Opentrons 8-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN1 P10 Electronic Pipettes, Opentrons 8-Channel GEN1 P10 Electronic PipettesOpentrons platform and workstation accuracy and precision performance is not validated with use of non-Opentrons tips.
Document
Opentrons OT-2 Tips, 20µL. Optimized for volumes 1µL to 20µL. Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.
Compatible with: Opentrons 8-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN1 P10 Electronic Pipettes, Opentrons 8-Channel GEN1 P10 Electronic PipettesOpentrons platform and workstation accuracy and precision performance is not validated with use of non-Opentrons tips.
